Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"micronucleus"”.
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THE AUTOPHAGIC RECEPTOR P62 MODULATES MICRONUCLEUS STABILITY
… micronuclear component(s) rather than the entire micronucleus. In line with this, I found that modulation of p62 levels has an impact on micronuclear membrane integrity. The molecular mechanism of this modulation is yet-to-be defined, and identifying p62 target will provide new insights in this …
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ASSESSING THE GENOTOXICITY OF TRICLOSAN IN TADPOLES OF THE AMERICAN BULLFROG, LITHOBATES CATESBEIANUS.
… for its simplicity and economy, is the micronucleus assay. A study was conducted assessing the genotoxicity of the widely used antimicrobial agent Triclosan to American Bullfrog tadpoles. Lithobates catesbeianus tadpoles were reared in glass aquaria containing ultra-high purity water and …
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Genoprotective effect of aspirin and ibuprofen in human lymphocyte cells. Effect of nano and bulk forms of aspirin and ibuprofen on lymphocytes from breast cancer patients compared with those from healthy females
… healthy females as a control using the Comet and micronucleus assays. Western blot analysis (WBA) was used to investigate the effect of these drugs on XRCC3 and p53 proteins, whereas QPCR was to evaluate this effect on p53, cox1 and cox2 genes. Two hundred fifty ng/ml of ibuprofen (NP and bulk) …
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In vitro chemically-induced DNA damage in cancer patients and healthy individuals. The effect of genotoxic compounds in cells from polyposis coli, colon cancer patients and healthy individuals.
… used in this study included the Comet assay, the Micronucleus assay, the Micronucleus ¿ FISH assay and the sister chromatid exchange assay. The food mutagens, PhIP and IQ, as well as titanium dioxide nanoparticles (TiO2 NPs) induced a dose dependent increase in the DNA damage and chromosomal …
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The analysis of radiation-induced micronuclei in peripheral blood lymphocytes for purpose of biological dosimetry
… the suitability of the cytokinesis blocked (CB) micronucleus assay as a biodosimetric method using lymphocytes irradiated in vivo. The approach adopted to achieve this was to estimate whole body doses by relating micronuclei yields in patients undergoing radiotherapy treatment with an in vitro …
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An Evaluation of the Occurrence of Micronuclei and other Nuclear Abnormalities in Fishes from the Great Lakes Basin, United States
… as a possible criterion for delisting. The micronucleus assay identifies genotoxic contamination by observing the presence of a micronucleus (MN), along with a primary nucleus within the cellular body. Nuclear abnormalities (NA) such as notching, lobes, blebbing, and binucleation are …
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Applying a new technique, the interferon gamma liposomal delivery system to improve drug delivery in the treatment of Lung Cancer
… was used to treat the lymphocytes in: Comet and micronucleus assays, Comet repair, Western blotting and real-time polymerase chain reaction (qPCR) were based on a preliminary test for the optimal dose. The lymphocytes from lung cancer patients presented with higher DNA damage levels than those of …
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Applying a new technique, the interferon gamma liposomal delivery system to improve drug delivery in the treatment of Lung Cancer
… was used to treat the lymphocytes in: Comet and micronucleus assays, Comet repair, Western blotting and real-time polymerase chain reaction (qPCR) were based on a preliminary test for the optimal dose. The lymphocytes from lung cancer patients presented with higher DNA damage levels than those of …
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Changes in buccal cytome biomarkers in relation to ageing and Alzheimer’s Disease.
… ageing syndromes such as AD and DS. A buccal micronucleus cytome assay was developed and used to investigate biomarkers for DNA damage, cell proliferation and cell death in healthy young, healthy old and young Down’s syndrome cohorts. Cells with micronuclei, karyorrhectic cells, condensed …
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Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells
… the clonogenic assay, the comet assay and the micronucleus assay. A novel co-culture system was then designed to study the bystander effect transmitted through the cell culture medium. The micronucleus assay was used as endpoint for the bystander studies. A bystander effect was observed.
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Genotoxic effects in human peripheral lymphocytes from healthy individuals and head and neck cancer patients after treatment with hydrogen peroxide and pembrolizumab liposome
… the healthy individuals by using the Comet and micronucleus assays. Western blotting and real-time polymerase chain reaction were performed to assess the potential of improving the repair mechanisms after treatment with naked Pembrolizumab and Pembrolizumab liposome. According to the results, …
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Micronuclei induction in AG01522 cells is independent of temperature and linear energy transfer
… dependence on the bystander effect. Using the micronucleus assay and primary human AG01522 fibroblast cells co-cultured as both the target and bystander cells, the incidence of micronuclei in both X-ray irradiated and alpha particle irradiated bystander experiments were ~2 fold over control …
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Application of in vitro erythropoiesis from bone marrow derived progenitors to detect and study genotoxicity
… to better predict human responses. The in vivo micronucleus (MN) assay is a robust toxicity test that assesses the genotoxic effect of drugs on adult bone marrow (BM) using the metric of genotoxic damage to the reticulocyte population in mice. An in vitro correlate to this assay might facilitate …
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Bystander Effects Due To Neutrons, And Low-Dose Hyper-Radiosensitivity To Gamma Rays In Human Cells Using Cytogenetics
… events were evaluated via the cytokinesis-block micronucleus assay in two normal human lymphoblastoid cell lines exposed to neutrons or gamma radiation. Micronuclei, nucleoplasmic bridges and nuclear buds were enumerated by recording the coincident presence of these endpoints within individual …
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Genotoxic effects of NSAIDs and hydrocortisone in bulk and nano forms in lymphocytes from patients with haematological cancers
… of healthy donors using the comet assay and the micronucleus assay. In order to determine any anti-cancer effects, these agents were also tested in peripheral blood lymphocytes in patients with haematological cancers. The glucocorticoid hydrocortisone was also evaluated for anti-oxidant capacity. …
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Genotoxic effects of oestrogens and nano-NSAIDs: Genotoxic effects of oestrogens in vivo and nano- and bulk forms of NSAIDs on blood samples from prostate cancer patients
… aspirin and ibuprofen, in the Comet and micronucleus assays, on whole blood taken from prostate cancer patients or volunteers. These were used because it is known that the sensitivity of DNA to genotoxins can be heightened in patients with cancer. Patients’ and volunteers’ blood was …
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Establishment of the Physical and Technical Prerequisites for the Determination of the Relative Biological Effectiveness of Low-energy Monochromatic X-rays
… endpoints clonogenic cell survival and micronucleus induction have been optimised for two established cell lines. The human mammary epithelial cells MCF-12A have been chosen due to the importance of RBE of soft X-rays for determination of risk from mammography. On the other hand, the use …
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The development of a liposomal form Secukinumab – an IL 17 pathway inhibitor in the treatment of psoriasis
… in its bulk and liposome form using comet and micronucleus assays on lymphocytes. The results from both assays have illustrated the safety of the drug and demonstrated the reduction of the DNA damage induced in both healthy individuals and patients with psoriasis. Secukinumab significantly …
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The development of a liposomal form Secukinumab – an IL 17 pathway inhibitor in the treatment of psoriasis
… in its bulk and liposome form using comet and micronucleus assays on lymphocytes. The results from both assays have illustrated the safety of the drug and demonstrated the reduction of the DNA damage induced in both healthy individuals and patients with psoriasis. Secukinumab significantly …
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Radiation-induced bystander fibroblasts both reduce and amplify micronuclei induction through the reciprocal bystander effect and the secondary bystander effect
… three types of secondary bystander cells. The micronucleus assay was used to analyze the amount of chromosome aberrations present. In the unirradiated secondary bystander population, an increase in percentage of binucleated cells with micronuclei from the background level to approximately the …
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