Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"microhomology"”.
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Inferring mechanisms of V(D)J recombination using statistical inference on high-throughput immune repertoire data
… nucleotide trimming, and the role of sequence microhomology. First, I identify genetic loci associated with modifying V(D)J recombination probabilities using genome-wide association inference and reveal individual differences in receptor generation. Next, I develop a probabilistic model of …
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The Role of 53BP1 in DNA Double-Strand Break Repair
… that resected DNA is preferentially repaired by microhomology mediated A-NHEJ. Furthermore, analysis of 53BP1 mutants shows that chromatin association, oligomerization, and N-terminal ATM phosphorylation sites are all required for preventing DNA end resection and joining as measured by …
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
… from compatible termini, an essential step in microhomology-mediated NHEJ. Artemis, a nuclease implicated in processing of DNA termini at a DSB during NHEJ, has been demonstrated to have both DNA-PK independent 5'-3' exonuclease activities and DNA-PK dependent endonuclease activity. Evidence …
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Fluorescent detection of homologous recombination reveals the impact of genetic, physiological, and environmental factors on genomic stability
… (HR), non-homologous end-joining (NHEJ) or microhomology-mediated end-joining (MMEJ). Here we use the Fluorescent Yellow Direct Repeat (FYDR) mouse to examine these pathways. Specifically, we crossed FYDR mice with mice lacking an essential NHEJ protein. Consistent with in vitro studies, we …
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Functional Characterisation of a RECQL4 Mutation in Rothmund-Thomson Syndrome
… in the homologous recombination (HR) and microhomology mediated end joining (MMEJ) pathways, providing evidence that RECQL4 disruption impacted replication-specific DNA DSB repair in particular. Additional RECQL4 reconstitution studies confirmed that the decreased HR repair was a result of …
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DNA repair proteins Metnase and PARP1 regulate DNA integration
… genome. Overexpression of Metnase suppressed microhomology-mediated DNA integration, supporting our hypothesis that Metnase promotes DNA integration through classical NHEJ (cNHEJ). In contrast to cNHEJ, alternative NHEJ (aNHEJ) utilizes a different set of core proteins to rejoin broken ends. …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… transformation of Tox3-HDR cassette with 50bp microhomology flanks was also successful with 25% HR efficacy. These data highlight a significant potential of CRISPR/Cas9 in expediting transgene-free gene knockouts in P.nodorum. However, its efficacy is dependent on targeted pathogen. Contrary to …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… transformation of Tox3-HDR cassette with 50bp microhomology flanks was also successful with 25% HR efficacy. These data highlight a significant potential of CRISPR/Cas9 in expediting transgene-free gene knockouts in P.nodorum. However, its efficacy is dependent on targeted pathogen. Contrary to …