Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"microfluidic technologies"”.
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Biophysical characterization of biomolecular condensates through microfluidic technologies
… of biomolecular condensates using microfluidic technology. These methods allow us to measure the stability of condensates through single-condensate zeta potential measurements, to probe the interactions between phase separating proteins and specific RNA sequences and other proteins …
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High throughput microfluidic technologies for cell separation and single-cell analysis
… separation technique using Dean-coupled inertial microfluidic sorter. Separation of cells by size before downstream assays might be beneficial in simplifying the system and facilitating the discovery of rare subpopulations through enrichment of cells with certain size range or cell cycle phase. By …
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New Microfluidic Technologies for Studying Histone Modifications and Long Non-Coding RNA Bindings
… patients and small lab animals are examined. Microfluidic technology offers advantages to utilize low-input starting material and for high-throughput. In this thesis, I developed novel microfluidic technologies to study epigenomic regulations, including 1) profiling epigenomic changes …
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Expanding the toolbox for precision medicine with silicon photonic microring resonators and microfluidic technologies
… treatment is not new to medicine, genomic technologies and therapies targeted to genetic drivers of disease have inspired an era of precision medicine. Obtaining molecular profiles of disease requires analyzing biological samples that present daunting analytical challenges with thousands of …
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Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification
Microfluidic technologies have gained wide acceptance in the past decade as diagnostics tools in clinical setting world-wide. This is primarily due to the fact that microfluidic technologies enable rapid, quantitative assays from small amount of physiological sample in an easy-to-use, portable …
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Microfluidic single-cell technologies for assaying lymphocyte interactions
… the development and implementation of novel microfluidic technologies for single-cell analysis of direct cell-cell interactions in immunology. By combining carefully designed weir-based hydrodynamic traps with a multistep cell loading procedure, the microfluidic devices capture and …
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Label-Free Microfluidic Devices for Single-Cell Analysis and Liquid Biopsies
… and prognostic tools. Recent advancements in technologies aiding cancer research have made significant strides, however a demand for a non-invasive clinically relevant point-of-care tools exists. To accomplish this feat, the desired instrument needs to be low-cost, easy-to-operate, efficient, …
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Microfluidic in vivo laser microsurgery screen for identification of compounds enhancing neural regeneration
… of automated, high-throughput optical and microfluidic technologies for screening C. elegans and demonstrates the production of a reliable system for screening over ten thousand animals. Using the screening system, femtosecond laser microsurgery was performed on thousands of animals …
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A General Purpose Programmable Microfluidic Platform for Screening and Optimization of Biological Assays
… consumables cost. Recently proposed droplet microfluidic technologies have the potential to substantially reduce assay costs by performing reactions using nanoliter volumes at very rapid rates. However, their incorporation into screening workflows is limited owing to various technological …
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Inertio-elastic focusing of bioparticles in a microchannel at ultra-high throughput
… manipulation of particles is often necessary. Microfluidic technologies based on viscosity-dominant flows have achieved this essential step in small-volume (</= 1 ml) fluid samples, while inertial focusing in microchannels has been used to process large-volume (-0(10 ml)) fluid samples. …
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Cell-cell communication in three dimensional microenvironments
… native tissue environments, optical tweezer and microfluidic technologies were used to place cells within defined areas of the culture environment. To provide three dimensional supports found in natural tissues, hydrogel scaffolds of poly (ethylene glycol) diacrylate and the basement membrane …
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Methods and technologies for high-throughput and high-content small animal screening
… elegans (C. elegans). First we present microfluidic technologies to rapidly isolate, immobilize, image and manipulate individual animals. These technologies include 1. a high-speed microfluidic sorter that can isolate and immobilize C. elegans in a well defined geometry for screening …
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Electrokinetic and acoustic manipulations of colloidal and biological particles
Recent advances in microfluidic technologies have enabled integration of the functional units for biological and chemical analysis onto miniaturized chips, called Labon- a-Chip (LOC). However, the effective manipulation and control of colloidal particles suspended in fluids are still challenging …
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Advancing Dynamic Imaging for Microfluidic Stimulated Tissues: Biology, Techniques, and Applications
… activities in intact tissues stimulated in a microfluidic device creates opportunities for studying major diseases such as diabetes and cancer. Despite a number of documented phenotypic traits of these diseases, the underlying pathophysiological mechanisms remain to be elucidated. Conventional …
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Novel Technologies for Evaluating Red Blood Cell Rheology during Blood Processing and in Sickle Cell Disease
… this dissertation also proposes the use of novel microfluidic technologies developed to (1) process stored RBCs to remove harmful residual plasma proteins, (2) investigate the effects of novel storage washing solutions on RBC morphology, and (3) aid in understanding the role both deformability and …
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A cell-based sensor of fluid shear stress for microfluidics
… physiological implications of FSS within microfluidic devices, or among competing microfluidic technologies).This thesis presents the first genetically encoded cell sensors that fluoresce in a quantitative fashion upon FSS pathway activation. A transcriptional sensor was chosen, meaning …
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Mixing at Low Reynolds Numbers by Vibrating Cantilevered Ionic Polymers
… been further highlighted with the rise of microfluidics. Based on the diminutive size of these devices, the Reynolds numbers are often less than 10, but have high Peclet numbers. Therefore, creating effective mixing is non-trivial and is a topic of active research, and is of paramount …
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Advanced Microfluidic Platforms for Studying the Stromal Compartment of Pancreatic Cancer Tumor Microenvironments
… the last decade, the synergistic combination of microfluidic technologies with 3D cultures have resulted in Organ-on-a-Chip (OOC) technologies that leverage the microscale manipulation of fluids to achieve precise spatiotemporal control of 3D tissues. However, many of these systems remain in the …
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A Microfluidic Approach For Investigating Thrombosis And Hemostasis: From Disease Models To Drug Testing
… plasma or isolated blood cells in the past. Microfluidic technologies offer a unique opportunity to study the mechanisms of hemostasis and thrombosis under physiologically relevant conditions. This thesis describes a microfluidic approach to model cardiovascular diseases and evaluate …
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Large-scale transgenesis and nerve regeneration in C. elegans
… high-throughput, optical, robotic, and microfluidic technologies for laser neurosurgery screening and transgenesis in C. elegans. Discovery of molecular mechanisms and chemical compounds that enhance neuronal regeneration can lead to development of therapeutics to combat nervous system …
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