Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"microfluidic diffusional sizing"”.
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Microfluidic diffusional sizing at the single molecule level
… this project presents the single-molecule microfluidic diffusional sizing (sm-MDS) platform and demonstrates its capabilities at performing diffusional sizing at picomolar sensitivity. Microfluidic diffusional sizing (MDS) is a robust, in-solution method to measure hydrody- namic radii …
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Physico-Chemical Characterisation of Disease-Related Protein-Protein Interactions in Solution
… those relevant under physiological conditions. Microfluidic diffusional sizing offers an alternative method for investigating protein-protein interactions and protein assembly under physiological conditions and has been applied to investigate a wide range of protein properties, functions, and …
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Investigating Protein Properties via Microfluidic Techniques
… of proteins, and protein interactions using microfluidic techniques. Chapter 1 provides a brief introduction to protein stability and identification as well as their interactions. Then, conventional techniques for studying protein systems are reviewed. Moreover, the principles, designs and …
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Characterisation of Alloantibody-Human Leukocyte Antigen Interactions to Improve Immunological Risk Assessment in Solid Organ Transplantation
… was explored. Lastly, using the principles of microfluidic diffusional sizing and Bayesian inference, microfluidic antibody affinity profiling (MAAP) was developed to enable in-solution, simultaneous determination of the KD and [Ab] of antibodies directly in patient serum samples. …
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Advances in understanding protein-lipid interactions through biophysical and bioelectronic approaches
… dichroism, transmission electron microscopy, microfluidic diffusional sizing and confocal microscopy. In our studies, we have aimed to incorporate natural membrane lipids in addition to the conventionally used synthetic lipids in order to increase the biological significance of our findings. …
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Biochemical and Biophysical Studies of the UPR Kinase PERK and its Interactors in Health and Disease
… techniques and biophysical methods, namely microfluidic diffusional sizing and Förster resonance energy transfer (FRET), to investigate interactions of PERK in solution and live cells. In-solution interaction studies confirmed recruitment of eIF2α by the unique insert loop situated within …