Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 93 for “"microdomains"”.
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Regulation of Ras signaling and oncogenesis by plasma membrane microdomains
… H-Ras-driven tumor progression, independent of microdomains. In the second phase of the study, we investigated the combinatorial use of two drugs currently either in active use as anti-cancer agents (Rapamycin) or in clinical trials (OTX008), as a novel strategy to inhibit H-Ras-driven tumor …
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Probing the formation of mammalian cell lipid microdomains with nanometer-scale imaging secondary ion mass spectrometry
Over forty years ago, Singer and Nicholson began studying the organization of the plasma membranes on mammalian cells. Since then, our understanding of this membrane has shifted from that of a homogeneous to a more heterogenous environment of proteins and lipids. The complexity of living cells have …
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Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
… rafts revealed the distinct identity of these microdomains at the Golgi complex rather than being the precursors of lipid rafts at the plasma membrane. Described in this thesis is a novel protein component of these microdomains. By use of degenerate PCR, combined with phage display, the …
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Single molecule tracking studies of solvent-swollen microdomains in cylinder-forming polystyrene-Poly (ethylene oxide) diblock copolymer films
Solvent swelling of block copolymer microdomains plays an essential role in the improvement of microdomain alignment by solvent vapor annealing and in chemical separations using block copolymer monoliths. Here, investigation of the effects of solvent swelling on the molecular permeability and …
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An Optical Flow Based Methodology for Visualizing Dynamic Sucellular [sic] Organiztion [sic] Demonstrated Through Profilin and Rho GTPase Microdomains
… of internally consistent signaling dynamics or "microdomains". Microdomains of GTPases match literature descriptions of signaling organization and in an optogenetic study were found to almost precisely match the perturbation footprint.
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Structural and Functional Characterization of GAPR-1, a Mammalian Plant Pathogenesis-related Protein in Lipid-enriched Microdomains of the Golgi Complex
During the characterization of lipid-enriched microdomains at the Golgi (GICs) (Gkantiragas, I. et al. 2001), a protein with an apparent molecular mass of 17 kDa was identified. Cloning and preliminary biochemical characterization identified a novel protein, GAPR-1, belonging to the superfamily of …
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Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… with each other to form tetraspanin-enriched microdomains, into which certain other receptors and signalling proteins are recruited and regulated. Tetraspanin microdomains have been implicated in a range of biological processes including cell signalling, adhesion, intracellular trafficking, …
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TORC2 and Eisosomes Are Spatially Interdependent, Requiring Optimal Level of Pi(4,5)P<sub>2</sub> for Their Integrity
… of the distribution of plasma membrane microdomains and how they are regulated. I aimed to investigate the dynamic interdependence between the protein complexes eisosome and TORC2, representing microdomains MCC and MCT, respectively. In this study, I reveal that the eisosome organizer …
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Fluorescence resonance energy transfer-based subcellular visualization of FAK/Src activation by chemical and mechanical environment
… at plasma membrane, which contains different microdomains called lipid rafts. In this thesis, specific Src/FAK biosensors based on fluorescence resonance energy transfer (FRET) were developed and further targeted to subcompartments of plasma membrane to visualize local Src/FAK signaling events …
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Physical and Functional Coupling of CFTR and PDE3A
… complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrated that phosphodiesterase type 3A (PDE3A) is physically and functionally coupled to cystic fibrosis transmembrane conductance regulator (CFTR). …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… indicated that GP-C complex clusters into microdomains of 120 nm size independent of other viral proteins. Clustering of GP-C into membrane microdomains is neither influenced by SSP myristoylation nor by the co-expression of the matrix protein do Z. Regions of plasma membrane containing Z …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… indicated that GP-C complex clusters into microdomains of 120 nm size independent of other viral proteins. Clustering of GP-C into membrane microdomains is neither influenced by SSP myristoylation nor by the co-expression of the matrix protein do Z. Regions of plasma membrane containing Z …
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Analysis of gamma secretase regulation by phosphatidylethanolamine using mammalian and Drosophila melanogaster in vitro and in vivo model systems
… within plasma membrane/early endosomes’ microdomains termed “rafts”. Indeed APP cleaving machinery, required for A? generation has been shown to reside in lipid rafts and the secretase activity on APP to depend on membrane cholesterol levels. Counterintuitive to the localization of …
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Field induced orientation of semicrystalline and non-crystallne block copolymer microdomain patterns
… casting' process orient amorphous cylindrical microdomains along the flow direction in a semicrystalline block copolymer. Subsequent crystallization of the crystalline block is significantly influenced by the pre-existing oriented amorphous cylindrical microdomains. The orientation of …
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Functional in vitro analyses of lipid raft-associated cathepsin b: implication for the invasive phenotype of inflammatory breast cancer
… of a proteolytic pathway to membrane </p> <p>microdomains. A functional role for cathepsin B was confirmed by </p> <p>the ability of CA074, a cell impermeable and highly selective </p> <p>cathepsin B inhibitor, to significantly reduce pericellular proteolysis </p> <p>and invasion by SUM149 …
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Characterization of the GBF1-Arf1 axis in enterovirus RNA replication
… membranes. We observed that Arf1 forms isolated microdomains in the replication organelles and that viral antigens are localized in both Arf1-depleted and Arf1-enriched microdomains. We investigated the viral protein composition of the Arf1-enriched membranes using peroxidase-based proximity …
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The Role of Membrane Domains In Protein and Lipid Sorting During Endocytic Traffic
… shows that association with ordered membrane microdomains is fully sufficient for PM recycling and that abrogation of raft partitioning leads to their degradation in lysosomes. These findings support a model wherein ordered membrane domains mediate PM recycling of membrane components from the …
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Effect of aging on metabolic status and hormone responsiveness in the ovary
… lipid structure including formation of raft microdomains may affect signaling by receptors involved in reproductive and metabolic functions. Insulin receptors, IGF-1 receptors and EGF receptors require highly ordered membrane microdomains for signal transduction. In general, plasma membranes …
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Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting
… Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in …
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