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Showing 1 to 14 of 14 for “"microcarriers"”.
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Exploring new applications for photophysically encoded microcarriers
… the applications of digitally encoded microcarriers for cell based assays. We succeeded to show that encoded microcarriers were suitable to grow cells on. Neither the coating at the surface of the beads (which facilitates the growth of the cells), nor the cells themselves hampered the …
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Extracellular Matrix-Derived Microcarriers as 3-D Cell Culture Platforms
… development and characterization of ECM-derived microcarriers for the in vitro dynamic culture and expansion of stromal cells for cell therapy and tissue engineering applications. To develop novel platforms for use in dynamic culture systems, initial work focused on applying electrospraying …
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Quantitative and mechanistic analysis of mammalian cell cultivation on microcarriers
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept of Nutrition and Food Science, 1984.
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Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers
… the sample with a mixture of differently encoded microcarriers, each carrying another probe which can specifically interact with one of the targets. This means that each target will bind to a differently encoded microcarrier. When the targets are caught, several methods exist to label those …
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Development of a Hybrid Carrier System based on DNA Origami Nanostructures and Layer-by-Layer Microcarriers
Die vorliegende Dissertation untersuchte die Kombination von DNA-Nanostrukturen, so- genannte DNA-Origami-Strukturen, mit Layer-by-Layer (LbL) Mikrotransportern zum Auf- bau eines verbesserten Medikamententrägersystems. Dies sollte die jeweiligen Vorteile der eigenständigen Systeme kombinieren um …
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High density animal cell culture systems using porous supports
Monolithic porous ceramic and porous polystyrene microcarriers were examined as supports for large scale mammalian cell culture. Vero cells and transformed baby hamster kidney (BHK) cells which produced human transferrin were grown in three different reactor configurations: a fixed bed ceramic …
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Novel carriers for oral delivery of hemophiliac factor IX
… improved the loading of hFIX within the microcarriers, which is critical for minimizing protein degradation. Optimizing the loading conditions by increasing the incubation time and using a reduced ionic strength buffer further improved the delivery potential of the microcarriers. The …
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Diseño de un sistema de dos fases acuoso para la recuperación de células madre a partir de tejido adiposo y escalamiento en biorreactor para su producción y uso en terapias regenerativas alogénicas
… producción, se comparó la expansión de iPSC en microcarriers y en agregados celulares, en cultivos en suspensión en spinner flask. Métodos: Se testearon los ATPS 5,6% PEG 8.000 – 7,5% DEX 500.000, 13.6% Ficoll 400,000 – 11.6% DEX 75,000 y 10% Ucon 5.100 – 10% DEX 75.000 para la purificación de …
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Bone Regeneration Potential of Mesenchymal Stromal Cells derived from a Clinically Relevant Rat Model of Osteoporosis
… for cell expansion on keratin protein coated microcarriers in static culture. Hair-derived keratin biomaterials have demonstrated their utility as carriers of biologics and drugs for tissue engineering. An optimal microcarrier was selected that demonstrated superior retention of the protein …
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A study of bioprocess designs for development of the myogenic secretome as a source of bioactive extracellular vesicles
… yield on poly (lactic-co-glycolic) acid microcarriers was 3-fold greater than that produced on a 2D surface. The yield of EVs from 175cm2 and 75cm2 surface areas following SEC isolation were 4-fold and 2-fold greater respectively, compared to those achieved using precipitation. Particles …
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A Bottom-Up Assembly Of Vascularized Bioartificial Constructs Using Ecm Based Microscale Modules
… tuned by blending collagen with or suspending microcarriers in the GAG solution. These capsule modules were seeded externally with vascular endothelial cells (VEC), and subsequently fused into tissue constructs possessing VEC-lined, inter-capsule channels. The microcapsules supported high …
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Development of a novel bioreactor and systems for suspension cell culture in biopharmaceutical production
… culture. Fixed-bed bioreactors and the use of microcarriers provide an alternative platform for their growth to produce biologicals. However, a high surface area is required to achieve the high cell density which leading to an elevated cost of production (mainly from microcarriers) and ensuing …
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PULMONARY DELIVERY OF PNEUMOCCOCAL VACCINE USING NANOCOMPOSITE MICROPARTICLE CARRIERS VIA DRY POWDER INHALATION
… encapsulating PspA4Pro were incorporated into microcarriers using L-leucine and spray dried to produce nanocomposite micro#particles (NCMPs) dry powder for inhalation. Parameters for the preparation of protein-loaded polyester poly (Glycerol Adipate-co-ω-Pentadecalactone), (PGA-co-PDL) NCMPs …
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A Systems Approach to Studying the Differentiation of Stem Cells Towards Hepatocytes
… by adapting the expansion stage onto Cytodex 3 microcarriers. Using the same culture volume as a tissue plate culture, we demonstrated the ability to achieve up to a 5-fold increase in cell number with a final cell density in the range of 4-5x106 cells/ml. These strategies show that the demand …