Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"microbioreactor"”.
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Integrated microbioreactor arrays for high-throughput experimentation
… design and development of integrated arrays of microbioreactors that can provide the oxygen transfer and control capabilities of a stirred tank bioreactor in a high-throughput format. The devices comprise a novel peristaltic oxygenating mixer and microfluidic injectors, which are fabricated …
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Microbioreactors for bioprocess development
… design, fabrication, and characterization of a microbioreactor integrated with automated sensors and actuators as a step towards high-throughput bioprocess development. In particular, this thesis demonstrates the feasibility of culturing microbial cells in microliter-volume reactors in batch, …
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Integrated microbioreactors for rapid screening and analysis of bioprocesses
… The ability to reproducibly sacrifice microbioreactors mid-run is exploited to demonstrate the feasibility of linking microbioreactors to genome-wide expression studies using DNA microarrays. The potential of the microbioreactor for investigating different growth conditions is confirmed …
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Development of new tools for the production of plasmid DNA biopharmaceuticals
… of this thesis, we designed a 1-mL fed-batch microbioreactor with online monitoring and control of dissolved oxygen, pH, and temperature, as well as continuous monitoring of cell density. We used the microbioreactors to scale down temperature-induced production of a pUC-based DNA vaccine …
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Application of MRI and low-field NMR in biopharmaceutical development
… the flow field within. The widely used ambr® 15 microbioreactor was studied using an imaging sequence that gave three-dimensional velocity data. By capturing velocity data in three-dimensions, it was possible to determine the turbulent kinetic energy within the ambr® 15, which has not previously …
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Automated hydrodynamic trap for single cell analysis in free solution
… conditions. To become the single cell microbioreactor (SCM), our trapping technique went through four generations. Our first generation was directed at sequencing organisms that were uncultivated. Rumen fauna from cattle, sheep, and reindeer consist of a heterogeneous cell mixture …
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Model-based Design and Control of Biopharmaceutical Manufacturing Processes
… for multiple bioreactor configurations including microbioreactor systems and stirred-tank bioreactors. Microbioreactors are a promising technology to accelerate biologic drug development, but can have localized dissolved oxygen (DO) concentrations that are damaging to the cells. To address this …
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Miniaturisation of pH Holographic Sensors for Nano-Bioreactors
Monitoring and controlling pH is of utmost importance in bioprocessing as it directly affects product yield and quality. Multiplexed experiments can be performed in nanobioreactors for optimisation of yield and cell heterogeneity in a relatively quick and inexpensive manner. In this thesis, a pH …