Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"microbial source tracking"”.
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Microbial Source Tracking in a Mixed Use Watershed in Northern Virginia
… levels of fecal indicator bacteria (FIB). The sources of fecal pollution in neighboring watersheds within the county were determined over the 12 months previous to this project by performing Antibiotic Resistance Analysis (ARA, a microbial source tracking protocol) on Enterococcus and …
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Examining Fluorescent Decay Rates For Microbial Source Tracking In South Carolina Blackwater Systems
<p>Richland County, South Carolina, has many watersheds appearing on the EPA's 303(d) list, including the Gills Creek watershed. This study interprets current water quality data within the Gills Creek watershed through the use of intensive target stream sampling for Escherichia coli (E. coli) as …
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Density-Based Clustering of High-Dimensional DNA Fingerprints for Library-Dependent Microbial Source Tracking
… have developed a new Library-Dependent Microbial Source Tracking method for identifying the host animals causing fecal contamination in local water sources. The Cal Poly Library of Pyroprints (CPLOP) is a database which stores E. coli representations of fecal samples from known hosts …
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Microbial Source Tracking of Bacteroides to Identify Human and Bovine Fecal Loading in McDaniel Lake
… health hazards to humans. By determining the source of fecal pollution, practices can be implemented to reduce the amount entering waterways. Bacteroides sp. have previously shown high host specificity allows for species-specific identification of fecal sources. Using quantitative real-time …
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Use of Escherichia coli for Microbial Source Tracking in a Mixed Use Watershed in Northern Virginia
… stream were chosen for E. coli monitoring and microbial source tracking (MST). MST was performed using antibiotic resistance analysis (ARA) and fluorometric analysis. Escherichia coli was chosen as the indicator bacterium for purposes of comparison with previous project data and because a large …
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Evaluation, Development and Improvement of Genotypic, Phenotypic and Chemical Microbial Source Tracking Methods and Application to Fecal Pollution at Virginia's Public Beaches
The microbial source tracking (MST) methods of antibiotic resistance analysis (ARA) and fluorometry (to detect optical brighteners in detergents) were used in the summers of 2004 and 2005 to determine the origins of fecal pollution at beaches with a past history of, or the potential for, high …
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Host Specific Microbial Source Tracking and Quantification Using the Partial 16SrRNA Gene Sequence of Bacteroides on the Wilson's Creek Tributary in Southwest Missouri
… problems. Many different point and non-point sources of fecal contamination influence Wilson's Creek in Southwest Missouri. Use of Escherichia coli for identification of fecal contamination in fresh water streams is the commonly used method approved by the EPA, but is limited in the ability to …
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Host Specific Microbial Source Tracking and Quantification Using a Partial 16SrRNA Sequence of Bacteroides on the Finley River, a Tributary of the James River in Southwest Missouri
<p>Microbial source tracking (MST) is a general term given to any process which aims to determine the source of microbial contamination found in an environmental (usually water) sample. These methods include both phenotypic and genotypic strategies. In this study, quantitative PCR was used to …
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Effects of 2015 earthquake on biological stability of water in Nepal
… in biological stability of the water sources compounded by poor sanitation practices in Kathmandu (severe earthquake damage) and Jhapa (no earthquake damage) using microbial source tracking and 16S rRNA sequencing. Samples from the same locations were taken before the earthquake in …
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CPLOP: The Cal Poly Library of Pyroprints
<p>To date, microbial source tracking (MST), i.e. determining the source of microbial contamination based on the specific strains observed in the environment, often uses methods that are time-consuming, expensive and not always reliable. The biology department at Cal Poly, San Luis Obispo has …
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Evaluation of agricultural beneficial management practices: Cattle access restriction to surface waters subsurface tile drainage management
… parasites and nutrients between treatments. Microbial source tracking indicated that livestock was the main source of fecal contamination in the stream. Greater bacteria and nutrient loads were observed in the unrestricted pasture system than the excluding pasture. Moreover, parasite and …
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Identification of fecal pollution sources of karst waters by a combination of Bacteroidales genetic marker, bacterial indicators, and environmental variables
… by a combination of Bacteroidales-based microbial source tracking (MST), traditional bacterial indicators, and environmental variables. Water samples from springs and wells were collected from Illinois, Wisconsin, Kentucky and Missouri quarterly. Quantitative PCR with seven primer sets …
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A multi-disciplinary approach to tracking the downstream impacts of inadequate sanitation in Central Appalachia
… of fecal contamination, they do not pinpoint the sources of such contamination. Source assessment in rural areas affected by untreated household waste might be improved with the human-specific, microbial source tracking marker HF183. This study attempted to quantify HF183 in two particular …
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A Comparative Study of Three Bacterial Source Tracking Methods and the Fate of Fecal Indicator Bacteria in Marine Waters and Sediments
… bacteria (FIB) in sand and sediments. The microbial source tracking (MST) methods antibiotic resistance analysis (ARA), Bacteroides human-specific primer test, and fluorometry were compared against the FIBs to determine how reliable each method was in detecting the presence of human fecal …
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Associations between Fecal Indicator Bacteria Prevalence and Demographic Data in Private Water Supplies in Virginia
… are often underserved in terms of financial resources and access to environmental health education. As the Safe Drinking Water Act (SDWA) does not regulate private water systems, it is the sole responsibility of the homeowner to maintain and monitor these systems. Previous limited studies …
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Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization
… In recent years there has been a shift towards microbial source tracking (MST). MST employs microbiological, genotypic, phenotypic and chemical methods to determine the source of fecal pollution in water bodies. Previous studies aimed at developing swine fecal biomarkers have had limited …
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Improving microbial fate and transport modeling to support TMDL development in an urban watershed
… efforts. Due to limited understanding of microbial fate and transport, predictions of FIB concentrations are associated with considerable uncertainty relative to other water-quality contaminants. By focusing on a data-rich instrumented urban watershed, this study aims to improve …
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Human Adenoviruses As Indicators Of Faecal Contamination And Pathogen Indices In The Environment And Shellfish
… and so should be used as part of a multi-tool source tracking system. Shellfish studies showed a low occurrence of HAdVs and HPyVs, probably due to poor virus recovery. Further studies are required to investigate this. The results of this study strongly supported the hypothesis that HAdVs and …
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Unveiling Origins and Dynamics of Fecal Indicator Bacteria in an Urban Creek
… (FIB) but are unable to address the complex sources of contamination contributing to the overall bacterial load. This study established chip-based digital polymerase chain reaction (cdPCR) techniques for microbial source tracking to unveil the origins of Escherichia coli (E. coli). Along with …
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Quantifying Potential Sources of Microbial Contamination in Household Drinking Water Samples
… the Biological Systems Engineering Department. Microbial and chemical source tracking were used to identify possible sources of contamination. A logistic regression was employed to investigate potential correlations between TC contamination and chemical parameters (e.g. NO3-, turbidity) as well …