Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"microarray hybridization"”.
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Characterization of Oligonucleotide Microarray Hybridization: Microarray Fabrication by Light-Directed in situ Synthesis – Development of an Automated DNA Microarray Synthesizer, Characterization of Single Base Mismatch Discrimination and the Position-Dependent Influence of Point Defects on Oligonucleotide Duplex Binding Affinities
The present thesis focuses on nucleic acid hybridization between free-floating target sequences and complementary end-tethered oligonucleotide probes on the surface of DNA microarrays. Hybridization experiments were performed on oligonucleotide microarrays (DNA Chips) which were fabricated with an …
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Floral induction in Brachypodium distachyon
… quantitative real time PCR, whole transcriptome microarray hybridization, constitutive gene expression, gene silencing via artificial microRNA expression, transcript immuno localization, protein immuno-detection, and reporter studies. From these experiments, it was determined that while some …
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Lysine acetyltransferase Gcn5-B regulates the expression of crucial genes in Toxoplasma and its function is regulated through lysine acetylation
… using chromatin immunoprecipitation coupled with microarray hybridization (ChIP-chip). We also demonstrate that GCN5b interacts with AP2-domain proteins, which are plant-like transcription factors in Apicomplexa. The interactions between GCN5b, AP2IX-7, and AP2X-8 were confirmed by reciprocal …
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CoryneRegNet : a reference database and analysis platform for corynebacterial gene regulatory networks
… knowledge, computer predictions and global DNA microarray hybridization experiments, has opened the way for the genome-wide analysis of transcriptional regulatory networks. The large-scale reconstruction of these networks allows the in silico analysis of cell behavior in response to changing …
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Statistical power for RNA-seq data to detect two epigenetic phenomena
… shows many benefits compared with traditional microarray hybridization-based and sequence-based methods.</p><p>In this dissertation, we focus on how investigating sequencing parameters may affect power of tests for detecting imprinting and/or AEI, and whether the current technology can provide …
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Liquid-on-liquid mixing for slide-based biological assays
… The major aim of this technique is improving the hybridization step in DNA microarrays. I printed microarrays and subjected them to hybridizations with varying stir rates, durations, and target DNA concentrations. My data suggest that the mixing produces at best a modest improvement in efficiency, …
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Investigation and application of methods for ancient DNA research
… for aDNA research such as genotyping microarrays. In this thesis, HTS and genotyping techniques were developed or refined to improve the application of aDNA to larger biological questions in evolution. This thesis successfully: a) describes an in-house hybridization capture system that …