Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"miRNA regulation"”.
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A Network Approach to Understanding miRNA Regulation in Adipose Tissue
… we employ two contrasting approaches to identify miRNAs that influence adipocyte phenotype. First, miRNAs were sequenced from 6 fat pads, primary preadipocytes, and primary mature adipocytes. Comparative analyses of abundance across adipose tissue type and stage of differentiation revealed a …
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Functional miRNA regulation of metastatic genes promotes tumor cell dissemination in non-small cell and small cell lung carcinomas
… has revealed their important contribution in the regulation of developmental programs across metazoan species. More recently, the role of alterations in expression of small noncoding RNAs, microRNAs (miRNAs) has emerged as a significant contributor to disease states, including each stage of tumor …
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Quantification of microRNA regulation and its consequences at the single cell level
MicroRNAs (miRNAs) are a class of small non-coding RNAs which play important roles in post transcriptional gene regulation. miRNAs regulate more than half of mammalian protein-coding genes. They have been found to participate in almost every cellular process and their dysregulation is associated …
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Understanding microRNA targeting with high-throughput biochemistry
MicroRNAs (miRNAs) are short RNAs that, in complex with Argonaute (AGO) proteins, guide repression of mRNA targets. miRNAs negatively regulate most mammalian mRNAs, and disruption of this regulation often results in severe defects at the cellular and organismal level. miRNA repression occurs …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… of organisms and biological processes. Each miRNA guides Argonaute (Ago) protein complexes to target and repress hundreds of genes in a sequence-dependent manner. To identify all targets of miRNA regulation, we performed UV crosslinking and immunoprecipitation (CLIP) of Ago complexes in mouse …
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Effects of Ca2+, microRNAs, and rosuvastatin on insulin-secreting beta cell function
… is crucial for beta cell function and its dysregulation leads to impaired insulin secretion. Therefore, understanding the central mechanisms involved in the regulation of exocytosis is essential to recognize possible targets for therapeutic intervention and treatment of T2D. In this thesis I …
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Examination of mammalian microRNAs by high-throughput sequencing
… in a wide range of cellular events. MicroRNAs (miRNAs) are an abundant class of small RNAs that post-transcriptionally repress expression of their target genes. Since miRNA targeting is based on its sequence, accurate and comprehensive annotation of miRNA genes is fundamental to understanding …
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Regulation of estrogen receptor-alpha mediated gene expression and endocrine resistance through estrogen receptor-alpha phosphorylation and micro-RNA in breast cancer
… estrogen receptor downregulators (SERDs), the regulation of ER transcriptional activity, and stimulation of cell proliferation in response to estradiol and SERMs/SERD. We showed that changes in ER affecting the phosphorylation status of the receptor greatly impact receptor function and …
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Regulation of Autoimmunity and Inflammation by microRNAs and Environmental Factors
… role of a class of noncoding RNAs, the miRNAs, during thymocyte development of the where central tolerance is established. We used the non-obese diabetic mouse (NOD) which is spontaneously developing T1D and have been described to have defects in the T cell maturation. By studying the …
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Neuronal FMRP: facilitating learning in a critical brain network for zebra finch song acquisition and regulating synaptic miRNAs spatio-temporally
… for vocalization, and in protein translation regulation via the microRNA (miRNA) pathway. Humans are vocal learners, meaning we must hear normal adult vocalization during a critical period in development in order to learn this vocalization properly. Because FXS has both impaired learning and …
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Genetic Predictors of Clinical Outcomes In Non-Small Cell Lung Cancer Patients
… approach focusing on two critical pathways - miRNA and inflammation pathways. MicroRNAs (miRNA) post-transcriptionally regulate around 30% of human genes. Polymorphisms within miRNA processing pathways and binding sites may influence patients’ prognosis through altered gene regulation. …
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The Epstein-Barr Virus-Encoded BHRF1 miRNAs Regulate Viral Gene Expression in Cis
… of latency genes including two microRNA (miRNA) clusters, the BHRF1 cluster and the BART cluster. The BHRF1 miRNA cluster is composed of three virally encoded primary miRNAs (pri-miRNAs) which are processed into four highly expressed mature ~22nt miRNAs that target mRNA for translational …
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Staphylococcal Enterotoxin B-Induced Acute Lung Injury:Mechanism and Treatment of Disease
… to edema. We also demonstrate the differential regulation of miRNAs in SEB-induced ALI, and identify two different miRNAs (miRNA155 and miRNA182), which play important roles in SEB-induced proliferation. Finally, we demonstrate the therapeutic effectiveness of resveratrol, a natural plant …
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Pharmacogenomics of sickle cell disease therapeutics: pain and drug metabolism associated gene variants and hydroxyurea-induced post-transcriptional expression of miRNAs
… by hydroxyurea treatment, particularly, miRNA expression. SCD patients were recruited from Yaounde Central Hospital and Laquintinie Hospital in Douala (Wonkam et al., 2018, Mnika et al., 2019 (b)), and recent migrants SCD patients from the DRC, recruited at the Haematology Clinic, Groote …