Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"miRNA biogenesis"”.
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Hypoxia Mediated Downregulation of Mirna Biogenesis Leads to Increased Tumor Progression
… tumor microenvironment leads to attenuation of miRNA biogenesis by downregulation of two key enzymes, Drosha and Dicer in cancer cells. Previous data from our laboratory had shown the clinical relevance of downregulated Dicer and Drosha in ovarian and other cancer types, but a clear mechanistic …
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Enhancement of Dicer as a personalized anticancer strategy
Normal miRNA production is essential for regulating protein expression and cellular fate. Misregulation of miRNA production is emerging as a key factor in the development of many disease states, including most forms cancer. A global downregulation of miRNA processing is common among cancers and has …
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SR proteins in microRNA/mRNA biogenesis
… involvement in regulating microRNA/mRNA biogenesis and their own expression. </p><p>Negative feedback regulation is a common mechanism maintaining the steady-state level of SR proteins (i.e. SC35 and SRp20), and several mechanism may be involved. In order to test if miRNAs are also …
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High intensity exercise induced regulation of the microRNA biogenesis pathway
… stress, cells rely on mitochondrial biogenesis – the process of creating new components of the mitochondrial reticulum. Recent studies suggest that microRNAs (miRNA) – small noncoding RNA molecules that fine-tune gene expression by silencing mRNAs through translational repression …
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Investigating MDM2 Regulation of Endothelial Cell Angiomir Biosynthesis in Response to High Glucose Treatment
MiRNAs are a class of short, non-coding RNAs that regulate gene expression at the post-transcriptional level. Dysregulation of miRNA production contributes to the pathogenesis of hyperglycemia-related complications. Although many studies have examined the effect of hyperglycemia on the expression …
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The Regulation of Microrna Biogenesis By Ribosome-Interacting Proteins
<p>MicroRNA (miRNA) are small, non-coding RNAs that affect gene expression through degradation of complementary mRNA targets or inhibition of translation. As they affect approximately 50% of all cellular processes, miRNA are tightly regulated by the cell through transcriptional and …
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A survey of fly and nematode small RNAs by deep sequencing
… they can be categorized as either microRNAs (miRNAs) or small interfering RNAs (siRNAs). In animals, miRNAs derive from characteristic hairpins in primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a pre-miRNA hairpin, …
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Egfr Modulates Microrna Maturation In Response to Hypoxia Through Phosphorylation of Argonaute2
<p>MicroRNAs (miRNAs) are generated by two-step processing to yield small RNAs that negatively regulate target gene expression at posttranscriptional level. Deregulation of miRNAs has been linked to diverse pathological processes, including cancer. Recent studies have also implicated miRNAs in …
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Characterization of Inducible SMC-specific Drosha Knockout Mice
… cells. Recent studies have shown that microRNAs (miRNAs), a new class of small RNAs that function as guide molecules in RNA silencing, regulate and maintain the differentiation state of SMCs. A transgenic animal model to study SMC-specific knockout of Dicer, one of two RNase III proteins in miRNA …
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Elucidating the function and biogenesis of small non-coding RNAs using novel computational methods & machine learning.
… of small non-coding RNAs and especially miRNAs, in C. elegans first and thereafter to almost all animals and plants, started to fill in the puzzle of a complex gene regulatory network present within cells. The aim of this thesis is to shed more light on the features and functionality of …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… expression post-transcriptionally. MicroRNAs (miRNAs) are a family of ~21-nt cellular RNAs that govern numerous pathological and physiological processes by mediating translational repression and deadenylation/decay of cognate mRNA. Dysregulation of miRNA expression have been associated with …
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MicroRNAs in early embryonic development : dissecting the role of miR-290 through miR-295 in the mouse
… of target mRNAs. Targeted deletion of the miRNA biogenesis machinery in the mouse has demonstrated essential roles for miRNAs during early development. In this thesis, I have examined the role of a family of miRNAs, miR-290 through miR-295 (miR-290 cluster), which are specifically expressed …
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Examination of mammalian microRNAs by high-throughput sequencing
… in a wide range of cellular events. MicroRNAs (miRNAs) are an abundant class of small RNAs that post-transcriptionally repress expression of their target genes. Since miRNA targeting is based on its sequence, accurate and comprehensive annotation of miRNA genes is fundamental to understanding …
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Genomics and regulatory functions of microRNAs and small silencing RNAs in Arabidopsis thaliana
… classes, the ~21-nucleotide (nt) microRNAs (miRNAs) and the diverse ~22-24-nt trans-acting and heterochromatic short interfering RNAs (siRNAs). In order to more extensively characterize the small RNA landscape in plants and to identify undiscovered miRNAs and siRNAs, we performed …
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Predicting molecular markers of development and regeneration through integrative analysis of high-throughput genomics data
… detection of longer ncRNAs was used to identify miRNA containing sites in the mouse genome. The use of EST data was determined to improve ncRNA prediction strategies during the development of this method by evaluating the performance of miRNA prediction by combining information regarding …
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Understanding Recognition of Primary MicroRNAs by Human Drosha/DGCR8
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by suppressing messenger RNAs. The RNase Drosha, as part of the Microprocessor complex, performs the initial processing step in miRNA biogenesis. Drosha, in complex with its partner protein DGCR8, must distinguish its RNA …
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The Role of MicroRNAs in Craniofacial Development
… been studied; however, the role of microRNA (miRNA) in mammalian palatal development has not been elucidated. miRNA are a class of short 19-21 nt noncoding RNA whose function is in the repression and attenuation of translation of their target mRNA. The function of specific miRNA is known in …
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Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer
… of ovarian tumors might be ablation of miRNA biogenesis. Here I conducted a broad-scale gain-of-function microRNA (miRNA) screen in 16 ovarian cancer cell lines to annotate the functional landscape present in such a chaotic genetic background. miRNAs function as multigenic perturbations …
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Characterizing the Expression Pattern of miR167-Regulated ZmArf3 and ZmArf30 in Maize Inflorescences
… dicer-like1, a key enzyme require for microRNA (miRNA) biogenesis in plants. MiRNAs small non-coding RNAs repress target mRNA expression and function in diverse developmental and physiological processes in plants. fzt plants have severe vegetative and reproductive defects, including highly …
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Diverse RNA processing pathways important for post-transcriptional gene regulation
… regulation of gene expression. Animal microRNAs (miRNAs) each target hundreds of mRNAs, which are recognized by pairing to nucleotides 2-7 of the miRNA. MicroRNAs mature through sequential RNase III cleavage of characteristic stem-loop precursors. Cleavage by Drosha defines the premiRNA hairpin, …
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