Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"miR-124"”.
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Membrane Bound Il21 Promotes Natural Killer Cell Expansion Through Mir 124-3P Mediated Regulation
… this may not be the only determining factor. MiRNAs, crucial regulators of post-transcriptional gene regulation could play a role in causing the observed differences in proliferation in these 2 expansion systems. The role of miRNA in NK cells biology is an emerging area of interest and their …
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Rest Regulatory Circuit Controls Distinct Oncogenic Properties of Glioblastoma Stem Cells Through Specific Micrornas
… we show that REST targets two microRNAs, miR-124 and miR-203 in High REST GSCs (HR-GSCs). Independent studies were carried out to determine the role of these microRNAs in HR-GSC derived brain tumors. Gain of function of either miR-124 or miR-203 in HR-GSCs leads to increased survival when …
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Investigating the Role of MicroRNAs in Regeneration and Axonal Pathfinding
… CNS in response to retinoic acid (RA). Using miRNA-Sequencing, I identified one neuronally enriched microRNA, miR-124, that was up-regulated in RA-induced regenerating CNS. Using PCR and in situ hybridization, I characterized its distribution in the snail CNS, and discovered it shared similar …
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Mechanisms of medulloblastoma vulnerability and new targeted therapies
… From computational predictions that identified miR-124-3p as a miRNA targeting MIZ1, we confirmed through luciferase assay in vitro that miR-124-3p binds to the 3’ untranslated region of MIZ1 and represses its protein levels. Over-expression of miR-124-3p impairs proliferation of group 3 cells, …
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The Roles of Nuclear Receptor NR4A1 in Cancer Cell Proliferation and Skeletal Muscle Differentiation
… </p> <p>One such mechanism is through microRNAs (miRNAs), which regulate gene expression by binding to the 3ʹUTR of target mRNA and effectively inhibit translation into protein. Prior to </p> <p>this study, no miRNAs had been identified to directly target <em>NR4A1</em>. By using luciferase …
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Visualization and manipulation of microRNA in neural cells
microRNA (miRNA) are small non-coding RNA, 21-23 nucleotides long. miRNA provide a new layer of regulatory control over gene expression programs. It is increasingly evident that miRNA are cell type and tissue specific. Many miRNAs have been identified to be highly abundant in certain regions of the …
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MiRNA influences in mesenchymal stem cell commitment to neuroblast lineage development
… study was to investigate if specific microRNAs (miRNAs) are responsible for neural induction, differentiation and fate specification and if they can induce MSCs to commit to a neuroblast and/or mature neuron cell lineage. The selected miRNAs were miR-107, miR-124 and miR-381, which respectively …
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Novel Small-RNA Mediated Gene Regulatory Mechanisms for Long-Term Memory
… We identified 170 distinct 21-23 nt sized miRNAs, 13 of which were novel and specific to Aplysia. Nine miRNAs were brain-enriched, and several of these were rapidly down-regulated by transient exposure to serotonin, a modulatory neurotransmitter released during learning. Two abundant, and …
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Deconvoluting Cell-Type Specific 3'UTR Isoform Expression in the Adult and Developing Cerebellum
… was concomitantly downregulated. We show that miR-124 specifically targets the long isoform of Memo1, which provides a mechanism for its downregulation during development. Our findings give insight into the roles for alternative polyadenylation in specific cell types and developmental stages, …
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Regulation of Mnk1 by p38α MAPK in Stress Mediated Translation Initiation
… the brain due to two neuron-selective microRNAs, miR-124 and -128. Next, we investigated the mechanism of p38α mediated Mnk1 activation to see if it differed from Erk1/2 mediated activation. Looking at the induced binding of Mnk1 to eIF4G, we found that the dissociation rate varies depending …
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MicroRNA and Epigenetic Controls of CD4+ T cells' Activation, Differentiation and Maintenance
… and interplays between two emerging mechanisms, miRNAs and epigenetic processes, in regulating CD4+ T cell-mediated responses. Using both gain- and loss-of-function genetic tools, we demonstrated that a miRNA cluster, miR-17-92, is critical to promote Th1 responses and suppress inducible Treg …
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MicroRNA gene expression in the zebra finch brain
… the zebra finch auditory forebrain. MicroRNAs (miRNAs or miRs) are important regulators of gene expression which may coordinate complex biological processes through post-transcriptional mechanisms. This dissertation aims to explore the potential roles of miRs in the genomic response to song. …
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Genome-Wide Decoding of mRNP and miRNA Maps
… RNA-binding proteins (RNABPs) and/or microRNAs (miRNAs). However, applying biochemical methods to understand such interactions in living tissues is major challenge. Here we developed a genome-wide means of mapping messenger ribonucleoprotein (mRNP) sites in vivo, by high-throughput sequencing of …
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Microglial dysfunction as an early susceptibility to Alzheimer's disease: the exacerbating role of developmental toxicity
… deleterious expression patterns for miR-124, miR-132, and miR-34a, critical to homeostatic neuroimmune maturation, were observed in Pb-exposed Tg females by PND 10 and 21, suggesting persistent, epigenetic re-patterning of the microglial response phenotype and limited adaptability. …
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THE ROLE OF P2X7R IN METHAMPHETAMINE-INDUCED BEHAVIORAL CHANGES AND MICROGLIAL EFFECTOR FUNCTIONS
… or microvesicles, and contain protein, mRNA and miRNA that alter the biological activity of target cells. I used electron microscopy, nanosight quantification, and confocal imaging techniques to confirm that METH causes the increase in primary human microglia-derived exosomes, which are around …
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Unraveling the Structure and Assessing the Quality of Protein Interaction Networks with Power Graph Analysis
Molecular biology has entered an era of systematic and automated experimentation. High-throughput techniques have moved biology from small-scale experiments focused on specific genes and proteins to genome and proteome-wide screens. One result of this endeavor is the compilation of complex networks …
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EMISSIVE PEPTIDE NUCLEIC ACIDS (PNAS): POTENTIAL TOOLS FOR INNOVATIVE DIAGNOSTICS AND THERAPEUTICS
… realize the objectives of the project. VI. Anti-miR124 PNAs for in vivo studies in ascidian embryos Besides the work on emissive PNAs, a part of this Ph.D. thesis was also dedicated to the study of anti-miR124 PNAs for in vivo studies using ascidian embryos as animal model. Anti-miR PNAs have …