Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"methylome"”.
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Exploring the methylome and transcriptome of young adult and aged OPCs
Remyelination is the restoration of myelin sheaths to denuded axons following demyelinating events, which occurs spontaneously in adult mammals, including humans. The principal cells which participate in remyelination are the Oligodendrocyte Progenitor Cells (OPCs). Similar to other regenerative …
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The Human Pancreatic Islet Methylome and Its Role in Type 2 Diabetes
Islet dysfunction is central to the development and progression of type 2 diabetes (T2D). Epigenetic modifications are essential for establishing and maintaining cell identity and function in normal circumstances. Exposure to adverse environmental factors may alter the epigenome, and result in …
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Evolution of the genome in myeloproliferative neoplasms and the methylome in blood
… neoplasms (MPNs) and identify changes in the methylome of blood with age and in the context of driver mutations. In the first study, serial sequencing of bulk samples from 30 patients with MPN coupled with clinical data show that clonal evolution is associated with disease transformation. …
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The Placenta Methylome: Novel Insights into DNA Modifications in the Human Placenta
… marked by global hypomethylation. The placental methylome has been shown to modulate developmental processes, intrauterine exposures, and placental function. However, the precise mechanisms underlying DNA methylation in regulating placental function and fetal development remains poorly studied. …
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Epigenetic variability and inheritance in East African cichlid fishes
… Here, I focus on whole-genome DNA methylation (methylome), a heritable and dynamic epigenetic mark that has been reported to be responsible for rapid and transmissible changes in phenotype in plants and mammals. In light with phenotypic plasticity related to diet adaptation, I hypothesise that …
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The Epigenetic Role of EGR1 during Postnatal Mammalian Brain Development
… types of cells. We hypothesize that brain methylomes are heterogeneously methylated across different types of cells and the transcription factors play key roles in brain methylome programming. To dissect brain methylome heterogeneity, in Chapter 2, we first focused on the identification of …
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Determining the Roles that DICER1 and Noncoding RNAs Play in Endometrial Tumorigenesis
… MeDIP/MRE) to characterize the transcriptome and methylome of endometrial cancer cells depleted of DICER1. Using a combination of computational and wet lab methods I showed that reduced DICER1 triggers an interferon response in cancer cells because of accumulation of pre-microRNAs that activate …
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The effects of virus-induced maternal inflammation on methylation patterns in skeletal muscle
… guanine, is associated with gene silencing. The methylome is established throughout gestation and may be influenced by maternal disease. Therefore, the influence of virus induced maternal inflammation on the methylome in developing muscles was investigated. The hypothesis was that the genome …
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Sex-dependent and Sex-independent Gene Regulation after Peripheral Nerve Injury
… and similarities in the transcriptome and DNA methylome of rodents after PNI.In my first Aim, I characterized the transcriptional similarities and differences between sexes and species after PNI. Based on the differentially expressed genes in mice and rats of both sexes, I generated a post-PNI …
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Targeted Epigenetic Editing to Increase Adult Pancreatic Β-Cell Proliferation
… in human β-cells, and that altering the DNA methylome in a targeted manner could improve proliferative capacity. To investigate this hypothesis, I sought to profile the β-cell across the human lifespan, and to develop tools that permit targeted DNA methylation modifications and efficiency in …
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The use of massively parallel sequencing to study the virome, epigenome and genome of canine and feline cancers
… (hDLBCL) cannot be faithfully reproduced in the methylome of cDLCBL, likely at a greater scale than DNA mutations, and neurogenic/neuroendocrine, HOX and Wnt pathways were epigenetically targeted. In canin osteosarcoma (OSA) we built upon the genome model by adding loss of heterozygosity data and …
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Investigating DNA Methylation Changes in a Preclinical Murine Model of Cutaneous Squamous Cell Carcinoma
… of this murine model and compare it to the methylome of human cSCC. We found that methylation changes in the murine cSCC tumors occur predominantly at regions of potential regulatory function, such as enhancers, promoters and intergenic regions of tumor suppressor genes that may be involved …
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CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING
… human embryonic stem (hES) cell and colon cancer methylome at single base resolution. I will present how an integrative analysis of DNA methylation, gene expression and histone modifications can identify the differentially methylated regions and genes involved in the regulation of cell …
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Integrative Approaches in Genomic Analysis: Advancing Epigenetic Prediction, Twas Methodology, and Cell-Type Deconvolution in Spatial Transcriptomics
… association studies (TWAS). Similarly, methylome-wide association studies (MWAS) have been developed to explore the relationships between DNA methylation and disease, as DNA methylation plays a key role in regulating gene expression. In Chapter 2 of this dissertation, we introduce MIMOSA …
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The Role of DNA Methylation in Defining the Vocal Learning Transcriptome of the Zebra Finch
… brain to explore mechanisms for establishing the methylome over development. Strikingly, I found that the de novo writer of DNA methylation, DNMT3A, is specifically upregulated in the RA relative to the surrounding arcopallium only at post-hatch day ~60, not earlier or in adulthood. These findings …
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DNA methylation: a model system for the study of ageing
… using single cell combined transcriptome and methylome sequencing (scM&T-seq). I found that with age there was increased global transcriptional variability and increased feature-specific methylome variability. These findings explain the loss of functionality of these cells with age. Lastly, I …
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Ocean acidification influences on physiology and epigenetics in the Pacific oyster (Crassostrea gigas)
… the effect of ocean acidification in C. gigas methylomes. To investigate the role of environmentally-responsive methylation in reproductive tissue, I analyzed gonad methylomes of female C. gigas exposed to low pH. A total of 1,599 differentially methylated loci (DML) were found in gene bodies. …
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Droplet-Based Microfluidics for High-Throughput Single-Cell Omics Profiling
… bisulfite sequencing libraries for DNA methylome profiling and allows production of BS library of 2,000-10,000 single cells within 2 d. We applied the technology to separately profile mixed cell lines, mouse brain tissues, and human brain tissues to reveal cell type heterogeneity. …
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… 168, for differences in cell growth, motility, methylome, and metabolome. We found that epigenetic methylation in B. subtilis regulates the metabolome and serves as rationale for continuing the investigation on whether epigenetic methylation can be used to optimize heterologous gene expression.
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Considering the impact of smoking on DNA methylation in Understanding Society
… than intensity in driving changes to the methylome caused by smoking. Furthermore, this differential methylation was reversed once a person had quit smoking and the degree of this decay increased with cessation years. Taking these findings, it was then possible to create two quantifiable …
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