Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"methylguanosine"”.
-
Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
<br/>The N-7 methylguanosine cap structure is conserved from yeast to man. It is essential for cell proliferation as it influences several steps in eukaryotic gene expression including transcription, pre-mRNA processing, RNA export and translation. The N-7 methylguanosine cap is added …
-
Identification and functional characterisation of RAM, a novel and essential component of RNA guanine-7 methylation
… expression in eukaryotes is dependent on the N-7 methylguanosine cap, located at the 5’ end of RNA pol II transcripts, which marks pre-mRNA for processing, stabilisation and translation initiation. The enzymes that catalyse the formation of the N-7 methylguanosine cap are recruited to RNA pol II …
-
Fluorescent Modifications of Transfer Ribonucleic Acids
… identified as either the X-nucleoside or the 7-methylguanosine in the variable loop of tRNA('Phe). The modified tRNA('Phe) can be aminoacylated to the extent of 20% as compared with the unmodified tRNA('Phe).
-
Human non-canonical translation initiation factors EIF4E2 and EIF4E3
… of a group of proteins that recognise the 7-methylguanosine cap at the 5' mRNA end to initiate translation. In humans, there are three isoforms, EIF4E1, EIF4E2 and EIF4E3. EIF4E1 participates in global translation and is considered canonical. EIF4E2 and EIF4E3 are non-canonical translation …
-
Eukaryotic Initiation Factor 3 Interactions with Structural Elements of Barley Yellow Dwarf Virus Untranslated Regions Reveal Details of a New Cap-Independent Translation Initiation Model
… plant virus that translates without using a 5′ 7-methylguanosine cap or a 3′ poly-adenosine tail, features that are required for canonical mRNA translation. BYDV’s non-canonical translation relies on RNA structures in the 5′ and 3′ untranslated regions (UTRs) to recruit eukaryotic initiation …
-
Site-specific chemical and topological modifications to augment mRNA therapeutic potential
… that possess an unnaturally-linked inverted 7-methylguanosine (m7G) cap. QRNAs substantially outperform conventional circRNAs, given the low translation initiation efficiency of IRES compared to cap-dependent initiation. Ultimately, our studies exploring the chemical and topological space of …
-
Investigating the cellular mechanisms that determine RNMT dependency in breast cancer cells
… anti-cancer therapeutics. The addition of the 7-methylguanosine cap (methyl cap) at the 5’ end of mRNA is essential for efficient protein synthesis and cell viability. In humans, RNA guanine-7 methyltransferase (RNMT) methylates the guanosine cap. RNMT expression is essential for cell viability …
-
The Effects of Polysomal mRNA Association and Cap Methylation on Gene Expression in <i>Trypanosoma brucei</i>
… will be examined in the context of the 7-methylguanosine cap attached to the 5´ end of the splice-leader. Interestingly, in addition to the capping enzymes identified in other eukaryotes, trypanosomatids have an additional guanylyltransferase and methyltransferase in the form of a …
-
The regulation and function of mRNA cap methylation in pluripotency and differentiation
The synthesis of the N7-methylguanosine cap at the 5’ end of pre-mRNA occurs co-transcriptionally and is catalysed by a series of enzymes including the N7 RNA methyltransferase (RNMT), which along with its recently discovered activating subunit RAM, methylates the cap. RAM, which contains an RNA …
-
Characterisation of RNA modifications in human cancer cells
Chemical modifications have been studied as key modulators of RNA biology for over 50 years. There are now 171 RNA modifications that have been discovered throughout the three kingdoms of life1. These chemical changes overlay the standard RNA information and have been linked to a wide range of …