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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"methylation analysis"”.
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Methylation analysis and diagnostics of Beckwith-Wiedemann syndrome
… performed to confirm diagnosis. In particular, methylation status analysis at two 11p15.5 imprinting control centres (IC1 and IC2) detects up to 80% of BWS cases. However, this is not without its limitations, especially in the detection of low-level mosaicism.<br/><br/>In order to evaluate the …
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Identification of Saliva Using DNA Methylation Analysis for Forensic Use
… recently been momentum in research to use DNA methylation for tissue identification.</p> <p>In the current study, five potential tissue-specific methylation markers were studied in order to identify locations in the genome that would differentiate saliva from other tissue types. Genomic DNA was …
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Methylation Analysis of Aerobacter Aerogenes Serotypes 2 and 54 Capsular Polysaccharides
Made available in DSpace on 2014-12-09T17:35:51Z (GMT). No. of bitstreams: 1 6801847.pdf: 4325293 bytes, checksum: 1609ee6ae2394e8de4c9d0addd0ee4d3 (MD5) Previous issue date: 1967
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DNA Methylation Analysis for Tissue and Age Determination for Forensic Human Identification
… identify relevant tissues, but tissue specific methylation markers are being examined as a new way for identifying tissue type. In addition, methylation markers are being investigated as a method to determine other relevant information about donors, such as the age of the individual.</p> <p>In …
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DNA methylation analysis and identification of a novel antisense transcript in the Peg3 imprinted domain
… First, we performed a systematic survey of DNA methylation status of the CpG islands in this region of the mouse, cow, and human genomes. We identified two previously unreported differentially methylated regions (DMR): one in the promoter region of mouse Zim3 and another in the promoter region …
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Dynamics and utility of cell-free DNA release following radiotherapy in prostate cancer and non-small cell lung cancer
… cell-free DNA (cfDNA) can be determined by methylation analysis and circulating tumour DNA identified by next-generation sequencing (NGS). To evaluate the dynamics of cfDNA during radical radiotherapy, total cfDNA was quantified in patients undergoing radiotherapy for prostate and lung …
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Structural studies of the capsular polysaccharides from some strains of Klebsiella and Cryptococcus neoformans micro-organisms
… including partial acid hydrolysis and methylation analysis, and the use of proton magnetic resonance spectroscopy and measurement of optical rotatory power to determine the nature of anomeric linkages. The polysaccharides from Klebsiella K4 and X.pnev.moniae ( oxytoca variant) have been …
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Some non-cellulosic b-D-Glycans from plant sources
… was obtained by employing techniques such as methylation analysis (involving use of gas liquid chromatography mass spectrometry), optical rotation measurements, mass spectrometry and n.m.r. spectroscopy on the original polysaccharides and on degraded products obtained by methods such as acid- …
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Chemical and spectroscopic studies of the capsular polysaccharides of some Klebsiella serotypes
… hydrolysis, products being identified by methylation analysis (involving the extensive use of gas-liquid chromatography coupled to mass spectrometry) and spectroscopic studies (mass and n.m.r.). During the course of these investigations it became apparent that the structures of the intact …
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Structural analysis of glycolipid-derived oligosaccharides from metabolically radiolabelled colorectal carcinoma SW1116 cells
This dissertation describes the analysis of the carbohydrate portion of glycosphingolipids from colorectal carcinoma cells, SW1116, by metabolically labelling the cells with radioactive monosaccharide precursors. SW1116 cells (1 x 10⁶) metabolically labelled with 222 μCi/ml of either …
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Temporal examination of DNA methylation profile reprogramming in the promoter region of PGC-1α during the progression of insulin resistance and type 2 diabetes mellitus in rodent models
… epigenetic modifications, in the form of DNA methylation, in the promoter region of peroxisome proliferator activated receptor gamma coactivator 1 alpha (PGC-1α), a known regulator of mitochondrial biogenesis. Following the initiation of a high fat diet, we observed significant genotypic (DNA …
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Characterising DNA methylation in tissue and liquid samples from patients with renal tumours
… Genomic alterations, such as mutational analysis, may have a role in RCC diagnosis and prognostication, but are unlikely to be sufficient alone due to low recurrence rates and significant intra-tumoral heterogeneity (ITH), which limit detection. Changes in DNA methylation are abundant and …
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Structural investigations of the polysaccharide gum exudates from Brabeiurn Stellatifoliurn and Grevillea Robusta
… was apparent, although techniques such as methylation analysis, Smith-degradation and partial acid hydrolysis revealed small differences in fine structure. Brabeium stellatifolium gum was examined in somewhat more detail. In a study of hydrolysis of the gum by acid the degradation process …
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Identification of epigenetically dysregulated genes in tumours that metastasise to the brain
… screen using literature review, bioinformatics analysis of 450K methylation data of primary breast tumours from The Cancer Genome Atlas (TCGA) and Genome-wide methylation analysis of metastatic brain tumors that originated from primary breast tumours were carried out. A candidate gene approach …
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IDENTIFYING EARLY STEPS IN THE DEVELOPMENT OF HBV ASSOCIATED HCC
… for earlier disease detection. Differential methylation analysis was also carried out on samples directly from TCGA and the Gene Expression Omnibus (GEO) to determine if the differentially expressed genes were potentially deregulated due to reversible epigenetic alterations. Enriched pathways …
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A clinicopathological and molecular genetic analysis of low-grade glioma in adults
… approaches such as IDH1 and IDH2 mutation analysis, MGMT methylation analysis, copy number analysis using array comparative genomic hybridisation and identification of differentially expressed miRNAs using miRNA microarray analysis. IDH1 mutation was present at a frequency of 71% in low …
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Using a Porcine Maternal Infanticide Model to Investigate the Inheritance of Human Puerperal Psychosis
… and then performed differential gene expression analysis. The results indicated that RNA-Seq technologies can be used with archival RNA samples, but using such samples introduces the risk of degradation-based bias. The substantial influence of outliers, confounding factors and sample size on the …
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Evolution Of Lactate Dehydrogenase Genes In Primates, With Special Consideration Of Nucleotide Organization In Mammalian Promoters
… We conduct a cross-tissue, cross-species methylation analysis, and determine that CpG methylation patterns across tissues appear similar between human and dwarf lemur; however, methylation levels across species vary.</p>
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The role of Arabidopsis thaliana heterochromatin component H2A.W in meiotic recombination
… modifications, including H3K9me2 and DNA methylation. Analysis of histone H2A variants via ChIP-sequencing has revealed that H2A.W is enriched in the heterochromatin. By combining cytogenetic, genetic and genomic assays, I have demonstrated that HTA7 is specifically involved in crossover …
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Towards elucidating the function of the oocyte Subcortical Maternal Complex: A multi-omic analysis of an Nlrp5-null murine model
… depends upon correct patterning of DNA methylation over imprinted domains during oogenesis. It was previously shown that oocytes deficient in the human SCMC gene *KHDC3L* had globally impaired methylation, thus suggesting a role for the SCMC in the establishment or maintenance of DNA …
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