Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"methionine sulfoxide"”.
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Methionine Sulfoxide Reductase and Ubiquitin-dependent Degradation of Alpha-synuclein
… on elevated dopamine and 14-3-3 levels in the methionine sulfoxide reductase A (MsrA) knockout mouse brain, as well as decreased αSyn degradation in MsrA KO yeast cells. Hence, this study will focus on the interactions among MsrA, αSyn degradation, 14-3-3 proteins, and Ub. Here we show that …
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Free methionine sulfoxide reductase and its contributions to environmental stress response in Komagataeibacter hansenii
… bacteria. In this thesis, the role of free methionine-R-sulfoxide reductase (MsrC) was investigated through comparative analysis of the wildtype (WT) K. hansenii, an msrC-deficient mutant (ΔmsrC), and a complemented strain using the shuttle plasmid pUCD2. The ΔmsrC mutation greatly affects …
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Methionine: oxidation state in processed foods and enzyme-catalyzed reaction with adenosine triphosphate
… the better set of conditions for measurement of methionine sulfoxide in food proteins. Recoveries of methionine, methionine sulfoxide, and methionine sulfone from base-hydrolyzed amino acid mixtures were, respectively, 89, 100, and 105% with the 2M NaOH conditions and 83, 90, and 98% with the 3M …
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Characterization of a carbon source-dependent, phytohormone-responsive, free methionine-(R)-sulfoxide reductase from Komagataeibacter hansenii
… thesis, the functional role of a putative free methionine sulfoxide reductase (MsrC) was characterized using a knockout mutant. The growth of the mutant was greatly affected by the presence of ethylene, abscisic acid and indole-3-acetic acid while oxidative stress increased BC production. …
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Neurochemical Measurements in Rodents that Model Huntingtion's Disease and Oxidative Stress
… in the 3NP treated rats. In a separate study, methionine sulfoxide reductase A (MsrA) mice, which model oxidative stress, have been reported to have chronically high dopamine levels relative to control mice. Additionally, these high levels parallel an increased presynaptic dopamine release when …
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Oxidative damage to recombinant proteins during production
… two methionine residues in the active site to methionine sulfoxide was sufficient for inactivation, and 50% of the antitrypsin produced under standard fermentation conditions was oxidized. Oxidative damage was linked to the dissolved oxygen concentration by experimentation and detailed modeling …
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Chemical and molecular properties of processed soy proteins
… of cysteic acid, and one isolate also contained methionine sulfoxide. None of the products were found to contain methionine sulfone. Length of time of acid hydrolysis was found to affect the measured essential amino acid content of processed soy flours, concentrate and isolates. Methionine and …
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INTRA-MITOCHONDRIAL INJURY DURING ISCHEMIA-REPERFUSION
… maintain homeostatis, living organisms have the methionine sulfoxide reductase system, which reduce both free and protein bound Met(O) back to methionine (Met) in the presence of thioredoxin. Oxidized Trx is inactive and unable to bind to ASK1 thereby activating ASK1 and causing cell death via …
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The study of two genes involved in the maintenance of intracellular redox potentials in Thiobacillus ferrooxidans
… of an E. coli trxA met mutant for conversion of methionine sulfoxide to methionine. The nucleotide sequence of a 1.1 kbp Hindiii-Psti T.ferrooxidans chromosomal DNA fragment containing the cloned trxA gene, was determined. An open reading frame (ORF) corresponding to the thioredoxin gene and part …
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Regulation of Mical Redox Post-Translationally-Driven F-Actin Cytoskeletal Dynamics
… oxidize actin on its conserved methionine-44 and methionine-47 residues, inducing both F-actin disassembly and altered actin polymerization. Our work has also revealed that this Mical-mediated actin regulatory process is reversible by a specific methionine sulfoxide reductase …
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The Oxidative Deamination of Sulfur Amino Acids by a Bacterial L-Amino Acid Oxidase
… 7.5 for these amino acids were L-homocysteine, L-methionine, S-adenosyl-L-homocysteine, S-adenosyl-L-methionine, L-djenkolic acid, S-ribosyl-L-homocysteine, S-adenosyl-L-homocysteine sulfoxide, L-homocysteine, methionine sulfoxide, L-cysteine and L-cystine.</p> <p>The particulate fraction …
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Structural characterization of the Voltage-Dependent Selective Anion Channels (VDACs)
Cysteine and methionine residues are unique amino acids in proteins for their property to undergo several redox reactions as part of their normal function. Mitochondria are the cell source of most chemical energy obtained as the end-product of the aerobic oxidation of glucose. Mitochondria are also …
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Characterization of the thioredoxin system in Methanosarcina mazei
… enzymes, such as ribonucleotide reductase, methionine sulfoxide reductase and glutathione peroxidase to name a few. It also plays an important role in combating oxidative stress and redox regulation of metabolism. Trx is a small redox protein, about 12 kDa in size, with an active site motif …