Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 33 for “"methanogen"”.
-
Characterizing methanogen and methanotroph diversity, abundance and activity within the Hampshire-Avon catchment
… sequencing, were applied to characterize methanogen and methanotroph community structure, abundance and activity in the Hampshire-Avon catchment, UK. Soil and river sediments were taken from sites across different underlying geologies based on their baseflow index (BFI); from low (chalk) …
-
Unexpected metabolic versatility discovered using new high-throughput genetic approaches in a model methanogen
Microorganisms known as methanogens produce most of the methane on Earth and areglobally distributed in anoxic (i.e., oxygen-free) environments such as freshwater and marine sediments, wastewater treatment facilities, and the digestive tracts of ruminants. Despite acknowledgement of their broad …
-
Characterization of a single TRAM domain RNA-binding protein from the Antarctic methanogen Methanococcoides burtonii
Methanococcoides burtonii, a methanogen that was isolated from Ace Lake, Antarctica, has proven to be a useful model for studying the molecular mechanisms of cold adaptation in Archaea. In Ace Lake, M. burtonii only experiences temperatures below 4 degrees and nucleic acid binding proteins have …
-
Computer-assisted generation and curation of genome-scale metabolic models with case studies in the methanogen genus Methanosarcina
Methanogenic archaea, organisms that make methane as a byproduct of their metabolism, play a critical role in the global carbon cycle and have potential as a source of renewable biofuels. As a result, there has been a great deal of interest in understanding how methanogenesis works. A wide array of …
-
Molecular biology approach to anaerobic digestion of marine biomass: Anaerobic digestion from marine materials. What makes a methanogen happy?
… of the dominant archaea and methanogens indicate that Methanomicrobiales and Methanosarcinales-related species could dominate sediment and sludge inoculated reactors, indicating the potential for utilisation of a diverse range of substrates. Results from the functional gene …
-
Organic geochemical biosignatures in alkaline Hydrothermal ecosystems
… We analyzed the 13C content of products of the methanogen Methanosarcina barkeri and found that fractionation varied strongly with substrate availability.These results inform our analysis of methanogen lipids from carbonates of the Lost City Hydrothermal Field. This ultramafic ecosystem produces …
-
An updated genome scale metabolic reconstruction of methanosarcina barkeri
Methanosarcina barkeri is a methanogen, meaning that methane is the reduced product for energy generation. M. barkeri can grow on several substrates including methanol, acetate, methyl amines and a combination of hydrogen and carbon dioxide. The Methanosarcina barkeri genome was published in 2006 …
-
Terminal anerobic interactions in a microbial association isolated from landfill
… by a hydrogen-oxidising species such as a methanogen or sulphate-reducing bacterium. The multi-stage chemostat was primarily used to facilitate spatial separation of the component species of the microbial association. Examination of the formation and subsequent utilisation of metabolites, …
-
Identifying ecological differentiation in microbial communities across taxonomic scales
… bacterial community to a single population of methanogenic archaea. At the broadest taxonomic scale, I used a whole-ecosystem disturbance along with 16S rRNA 454-pyrosequencing to identify ecologically relevant distinctions among taxonomic groups defined at various taxonomic scales. The …
-
Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
Electron transport during methanogenesis in the freshwater methanogen Methanosarcina barkeri is known to utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic …
-
Characterization, purification, and DNA sequence analysis of a novel ATPase of the archaebacterium Methanococcus voltae
… ATPase activity was detected in the methanogen Methanococcus voltae. The ATPase was inhibited by vanadate, a characteristic inhibitor of the P type ATPases. The enzyme activity was also inhibited by diethylstilbestrol. However, it was insensitive to …
-
Expanding the genetic toolbox for methanogenic archaea and investigating ferredoxin redox circuit formation in Methanosarcina acetivorans
Methane-producing archaea (methanogens) occupy a number of anoxic niches from soil sediments to the gastrointestinal tracts of numerous animals. In these anaerobic environments methanogens have specialized in converting simple substrates like carbon dioxide (CO2), formate, and acetate to methane …
-
Sulfite reductase and thioredoxin in oxidative stress responses of methanogenic archaea
Methanogens are a group of microorganisms that utilize simple compounds such as H₂ + CO₂, acetate and methanol for the production of methane, an end-product of their metabolism. These obligate anaerobes belonging to the archaeal domain inhabit diverse anoxic environments such as rice paddy fields, …
-
Developments in anaerobic digestion modelling
… kinetic constants of the acidogens, acetoclastic methanogens, acetogens and hydrogenotrophic methanogens. This required coding into WEST® (MikebyDHI, 2016), the platform on which PWM_SA runs, a six-in-series completely-mixed AD system with a solids retention factor for each digester that retained …
-
Comparative analysis of primate hydrogenotrophic microbiota
… critical to the functioning of this ecosystem. Methanogenesis by methanogenic Archaea and sulfate reduction by sulfate reducing bacteria (SRB) are the major hydrogenotrophic pathways in the human colon. Hydrogen metabolism by these microbes has an important impact on the colonic health. …
-
Functional and mechanistic elucidation of peptide backbone thioamidation
… (MCR) and the ribosome. Genetic studies in the methanogen Methanosarcina acetivorans have linked the thioamide in MCR with ycaO and its neighboring gene tfuA, which has no known function. In this thesis, I report the in vitro thioamidation of an MCR-derived peptide using methanogenic YcaOs and …
-
Microbial communities, activities, and metabolisms in Earth’s subseafloor and analog oceanic environments
… methanotrophs were detected, though microbial methanogenesis was not observed. The second part of the thesis focuses on cultivation-based approaches to characterize newly isolated microbes and their responses to high-pH and low-energy conditions. Chapter 4 investigates Methanocalculus …
-
Molecular characterization of microbial communities in small-scale biodigesters using whole genome shotgun metagenomic approach
… hydrolysis, acidogenesis, acetogenesis, and methanogenesis, resulting in digestate and biogas production. Advances in metagenomic techniques, surpassing traditional culture-based methods, have enabled a deeper characterization of the microbial populations involved in this process. However, a …
-
Coalbed gas origin and distribution in the southeastern Illinois Basin
… uplift and brine dilution initiated microbial methanogenesis. Distributions and compositions of gases in biogenic systems are governed by numerous factors including salinity, microbe-accessible porosity, and coal permeability. In contrast, thermogenic gas systems are primarily controlled by …
-
The use of lactic acid bacteria as methane mitigation strategies in ruminants
… mitigation potential of LAB, several species of methanogens (methane producing microorganisms) were cultivated. In Chapter 3, a total of 6 methanogenic species of both human and ruminant origin were cultivated. Methanobrevibacter ruminantium and Methanobrevibacter gottschalkii were chosen for …
Page 1 of 2