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Showing 1 to 11 of 11 for “"methanococcus maripaludis"”.
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Unexpected metabolic versatility discovered using new high-throughput genetic approaches in a model methanogen
… mechanisms contributing to the fitness of Methanococcus maripaludis as a model for methanogenic archaea in general. In this introduction, I will discuss our current understanding of methanogenesis and the metabolic capabilities of hydrogenotrophic methanogens while also highlighting gaps in …
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Investigating the Distribution and Biosynthesis of Modified F<sub>430</sub> Cofactors in Methanogenic and Methanotrophic Archaea
… in several methanogenic species, including Methanococcus maripaludis, Methanosarcina acetivorans, and Methanocaldococcus jannaschii. A novel variant reported here has an exact mass of 1008.3478, a similar absorption spectrum as unmodified F<sub>430</sub>, and associates with purified Mcr …
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Investigating the early events in proteasome assembly
… we have shown that recombinant α subunits from Methanococcus maripaludis also have a tendency to self-assemble into higher order structures when expressed in E. coli. Using a novel cross-linking strategy, we were able to establish that these higher order structures were double α rings that are …
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Growth and Survivability of Microorganisms at Martian Temperatures and Pressures
… barkeri, Methanobacterium formicicum, Methanococcus maripaludis, and Methanothermobacter wolfeii) to survive and/or grow under 1) low-pressure conditions and 2) freeze/thaw cycles. Low pressure studies include both survival and active growth experiments conducted between 7 mbar (the …
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Recombinant Expression and Assembly of Methyl Coenzyme-M reductase
… study of various ANME MCRs in the methanogen, Methanococcus maripaludis. We report the successful expression and purification of an ANME-2d MCR, marking a significant step toward the development of a heterologous MCR expression system. Additionally, our attempts to purify various recombinant …
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Molecular Characterization of Mth203 protein
… complexes. The Mth203 homologue (Mmp0457) in Methanococcus maripaludis has been co-purified with one of the MCM proteins (McmA) in this organism. Thus, it is likely that this protein interacts with the replicative machinery. The aim of this study was to further characterize Mth203 structure …
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Molecular characterization of Mth203 protein
… complexes. The Mth203 homologue (Mmp0457) in Methanococcus maripaludis has been co-purified with one of the MCM proteins (McmA) in this organism. Thus, it is likely that this protein interacts with the replicative machinery. The aim of this study was to further characterize Mth203 structure …
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Computer-assisted generation and curation of genome-scale metabolic models with case studies in the methanogen genus Methanosarcina
… well developed in model methanogens such as Methanococcus maripaludis, Methanosarcina acetivorans and Methanosarcina barkeri. Methanosarcina species are particularly attractive model organisms for methanogenesis due to their wide substrate utilization capabilities (compared to other …
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Biochemical and structural characterization of radical SAM enzymes Elp3 and viperin
… tRNAs and tRNAArg were isolated from yeast, Methanococcus maripaludis (Euryarchaeota) and Sulfolobus acidocaldarius (Crenarchaeota). Neither cm5U nor mcm5U/ncm5U (modification in eukarya) was detected in the archaea; however, two new substances (549 Da and 454 Da) were detected. Further …
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Assembly and substrate recognition properties of human CCT subunits of the TRiC chaperonin
… whether the archaeal group II chaperonin from Methanococcus maripaludis (Mm-Cpn) could recognize a variety of H[gamma]D-Crys mutants. These mutations were in regions of the protein that could act as recognition signals of substrate - unpaired aromatics, domain interface, and buried core …
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Integrating manual and automated metabolic engineering methods
Made available in DSpace on 2016-07-07T20:27:15Z (GMT). No. of bitstreams: 4 RICHARDS-DISSERTATION-2016.pdf: 3860821 bytes, checksum: cbaf30718ce7594d7f76859b49a220de (MD5) Richards_Matthew.docx: 2576729 bytes, checksum: e2a1076a288d7d31c646df244282d03a (MD5) LICENSE.txt: 4213 bytes, checksum: …