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Showing 1 to 20 of 26 for “"methane monooxygenase"”.
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Structural and functional models for methane monooxygenase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1991.
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Structural enzymology of soluble methane monooxygenase protein-protein interactions.
Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that activates molecular oxygen, breaks the 105 kcal/mol C-H bond of CH4, and inserts one atom of O to create methanol at ambient temperature and pressure. This feat of catalytic prowess requires all three protein components of …
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Mutagenic studies into the catalytic versatility of soluble methane monooxygenase
Soluble methane monooxygenase (sMMO) is a multicomponent bacterial enzyme that catalyzes the oxidation of methane to methanol, as well as oxidizing many other adventitious substrates. A number of mutagenic studies were carried out on the sMMO enzyme of Methylosinus trichosporium OB3b in order to …
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Mechanistic and physical studies of methane monooxygenase from Methylococcus capsulatus (Bath)
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1995.
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Structural studies of the hydroxylase component of methane monooxygenase from methyloccous capsulatus
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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Structural and mutagenesis studies of soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)
… for the 27 kDa flavin binding domain of soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath) was solved by NMR spectroscopy. The structure consists of a two domains, an FAD binding domain with a six-stranded antiparallel β-barrel and one α-helix, and an NADH binding domain …
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Studies of the soluble methane monooxygenase : heterologous expression and reactions with nitric oxide
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1999.
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Electron transfer and protein engineering studies of the soluble methane monooxygenase from Methylococcus capsulatus (Bath)
… of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus capsulatus (Bath) ... Chapter 3. Expression and Characterization of Ferredoxin and Flavin Adenine Dinucleotide-Binding Domains of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus …
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Bioinorganic hydrocarbon oxidation : mechanistic and kinetic studies of the soluble methane monooxygenase from Methylococcus capsulates (bath)
… by Metalloproteins as Exemplified by the Soluble Methane Monooxygenase -- Chapter 2. Small Molecule Binding to the Mixed-Valent Diiron Center of Methane Monooxygenase Hydroxylase from Methylococcus capsulatus (Bath) as Revealed by ENDOR Spectroscopy -- Chapter 3. An EPR Study of the Dinuclear Iron …
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Electrochemistry of soluble methane monooxygenase on a modified gold electrode : implications for chemical sensing in natural waters
… explored the possibility of using the soluble methane monooxygenase (MMO) enzyme, a three-component enzyme which catalyzes the oxygenation of methane and other substrates, to design a methane sensor for use in natural waters. Such a sensor would be useful for monitoring lake biogeochemistry, …
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Mechanistic studies of electron transfer, complex formation, C-H bond activation, and product binding in soluble methane monooxygenase
Chapter 1. Soluble Methane Monooxygenase: Activation of Dioxygen and Methane The mechanisms by which soluble methane monooxygenase uses dioxygen to convert methane selectively to methanol have come into sharp focus. Diverse techniques have clarified subtle details about each step in the reaction, …
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Exploring the reactivity of bacterial multicomponent monooxygenases
… The Reactivity of Bacterial Multicomponent Monooxygenases Bacterial multicomponent monooxygenases constitute a remarkable family of enzymes that oxidize small, inert hydrocarbon substrates using molecular oxygen. Three or more protein components are required for the timely reactions of …
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Understanding orchestrated chemical reactions in toluene/o-xylene monooxygenase from pseudomonas sporium OX1
… in the family of bacterial multicomponent monooxygenases (BMMs), soluble methane monooxygenase (sMMO) and toluene/o-xylene monooxygenase (ToMO), are described. The functions and structures of the three or four components of sMMO and ToMO are summarized. Distinctly different dioxygen …
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Towards a mechanistic understanding of contaminant attenuation and greenhouse gas emissions in open-water engineered wetlands
… and antibiotic (trimethoprim). Next, methane-oxidizing activity, catalyzed by the particulate methane monooxygenase as confirmed by field metatranscriptomics and inhibition microcosms, stimulated the biotransformation of sulfamethoxazole, an antibiotic that was highly recalcitrant …
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Dioxygen activation and substrate hydroxylation by the hydroxylase component of toluene/O-xylene monooxygenase from pseudomonas sporium OX1
… components of the bacterial multicomponent monooxygenases activate dioxygen at structurally homologous active sites. Catalysis requires the management of four substrates: electrons, protons, dioxygen, and hydrocarbons. Protein component complexes control the delivery of these substrates to …
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Structural investigations of hydroxylase proteins and complexes in bacterial multicomponent monooxygenase systems
… monooxgenases (BMMs) such as toluene/o-xylene monooxygenase (ToMO), phenol hydroxylase (PH), and soluble methane monooxygenase (sMMO) catalyze hydrocarbon oxidation reactions at a carboxylatebridged non-heme diiron center common to many systems in biology, as discussed in the first and …
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Molecular genetics and microbiology of bioremediation using methane-oxidising bacteria
Methanotrophic bacteria can grow on methane as their sole source of carbon and energy. Methylosinus trichosporium OB3b is a Gram negative, methanotrophic bacterium, which can convert methane to methanol by either particulate (pMMO) or soluble (sMMO) methane monooxygenase. The sMMO comprises three …
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Patterns, Processes And Models Of Microbial Recovery In A Chronosequence Following Reforestation Of Reclaimed Mine Soils
… lower relative abundances of methanotrophs and methane monooxygenase at previously-mined sites compared with unmined sites, which supports previous observations that ecological function of methane sink provided by many forest soils has not recovered after 30 years. Because of the difficulty …
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Bioremediation of hexavalent chromium using gram-negative bacteria.
… media. Phenylacetylene, an inhibitor of soluble methane monooxygenase, completely inhibited Cr (VI) removal. The results reveals that pMMO, sMMO or other enzymes that induced by copper were involved in reducing or otherwise removing Cr (VI). The di-heme cytochrome c peroxidase is also a possible …
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