Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"metatranscriptomic"”.
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Metatranscriptomic and physiological analyses of proteorhodopsin-containing marine flavobacteria
Proteorhodopsin (PR) is a seven-helix integral membrane protein that uses retinal as a chromophore. PRs transport protons from the cytoplasmic (CP) to the extracellular (EC) side of the cell membrane utilizing the energy from light. Since PR was first discovered in marine Gammaproteobacteria, …
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Metatranscriptomic analysis of community structure and metabolism of the rhizosphere microbiome.
… is about the plant microbiome. Here, comparative metatranscriptomics was used to determine taxonomic compositions and metabolic responses of microbes in soil and the rhizospheres of wheat, oat and pea. Additionally a wild-type oat was compared to a mutant (sad1) deficient in production of …
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Unearthing the mechanisms of the mycorrhizal-bacterial symbiosis in plant roots using a metatranscriptomic approach
… dependencies on the host fungi. In this work, a metatranscriptomic approach is applied in order to determine the transcriptional mechanisms underlying this multilayered symbiosis. Different mycorrhizal fungal species were found to be colonized by distinct communities of bacteria, and the study …
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Effects of nitrogen-fixing symbiotic cyanobacteria on the microbial ecology of the coral, Montastraea cavernosa
… using sequencing of ribosomal RNA PCR amplicons, metatranscriptomic sequencing of rRNA and mRNA from the holobiont, and experimental tests of various fitness metrics. The cyanobacterial symbionts appear to be diverse, but many are Pleurocapsa-like and related to nitrogen-fixing cyanobacterial …
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Microbial metatranscriptomics : towards understanding microbial gene expression and regulation in natural habitats
… and applications of deep sequencing-based metatranscriptomics, for profiling community transcriptomes on spatial and temporal scales. Several findings and relevant hypotheses have emerged from this work. I show that transcripts of house-keeping genes necessary for the maintenance of basic …
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Interactions Between the Odorous House Ant (Tapinoma sessile) and RNA Viruses: a Multi-Faceted Approach
… four novel viruses in T. sessile using metatranscriptomic analysis, investigated their infectivity, and explored how colony social organization influences viral infection patterns. Inoculation experiments confirmed active infection in T. sessile colonies, suggesting trophallaxis as a …
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Decoding divergence in marine protistan communities: from strain diversity to basin biogeography
… 4 and 5). I applied these tools to a three-year metatranscriptomic dataset from Cape Cod Bay to investigate a recent emergence of a summer occolithophore population in the 20-year time series, tracking shifts in nutrient physiology to identify potential bottom-up controls (Chapter 6). This …
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Analysis of microbes in Greenland ice cores from periods of high and low atmospheric carbon dioxide levels
… distinct past periods of time. Metagenomics and metatranscriptomics offer means to perform comprehensive analyses of microbial genes and taxa within the ice. During the past 400,000 years, fluctuations in atmospheric carbon dioxide levels have directly coincided with temperature fluctuations. …
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Examination of the nematostella vectensis holobiont by comparative bacterial genomics and metatranscriptomics
… antibiotic resistance protein. Whole anemone metatranscriptomic analysis based on BLASTx annotation of sequenced transcripts revealed bacterial expression of housekeeping genes such as those for replication, ribosomal structure and ATP-synthesis dominated by Proteobacteria, in particular …
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Decoding divergence in marine protistan communities: from strain diversity to basin biogeography
… 4 and 5). I applied these tools to a three-year metatranscriptomic dataset from Cape Cod Bay to investigate a recent emergence of a summer coccolithophore population in the 20-year time series, tracking shifts in nutrient physiology to identify potential bottom-up controls (Chapter 6). This …
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Defining the ecological and physiological traits of phytoplankton across marine ecosystems
… assays or metatranscriptome studies. Using a metatranscriptomic approach, the role of resource partitioning in the coexistence of two closely related diatom species in an estuarine system was examined. This study demonstrated that co-occurring diatoms in a dynamic coastal system have apparent …
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Iron and the ecology of marine microbes
… community gene expression dynamics. Using a metatranscriptomic approach I showed that in that environment, the impact of iron was tightly connected to the supply of other limiting macronutrients, and seems to mostly affect photosynthetic organisms. This initial study paves the way for more in …
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Defining the ecological and physiological traits of phytoplankton across marine ecosystem
… assays or metatranscriptome studies. Using a metatranscriptomic approach, the role of resource partitioning in the coexistence of two closely related diatom species in an estuarine system was examined. This study demonstrated that co-occurring diatoms in a dynamic coastal system have apparent …
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Structural dynamics of community gene expression In a freshwater cyanobacterial bloom over a day-night cycle
Studies of community gene expression, or metatranscriptomics, provide a powerful approach for quantifying changes in both the taxonomic composition (structure) and activity (function) of complex microbial systems in response to dynamic environmental conditions. We have used next-generation Illumina …
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Investigating physiological collaborations between a lower termite and its symbionts
… of microbes in this system by taking a global, metatranscriptomic approach. Differential expression analysis identified a bacterially-encoded amidohydrolase that may be important for anti-fungal defense.</p> <p>Overall, this project has significantly expanded our perspective on the importance of …
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Shotgun metagenomics-based exploration of kefir microbiomes
… be applied, to the analysis of metagenomic and metatranscriptomic data relating to fermented foods. In addition, we critically analyse a number of studies of fermented foods in which these tools have previously been applied to highlight the insights that these approaches can provide. Chapter 2 …
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Microbial nitrogen-cycling and cooperation in the terrestrial subsurface
… for anammox. This trend was confirmed with metatranscriptomic data, which revealed the co-occurrence of aerobic (nitrification, including ammonia oxidation) and anaerobic (denitrification, nitrite-dependent anaerobic methane oxidation, and anammox) processes in dysoxic groundwater (0.3–1.1 …
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The functional role of root-associated microbiome and metabolome of myrothamnus flabellifolia
… rhizosphere and subsequently to the endosphere. Metatranscriptomic analysis has revealed crucial insights into the dynamics of plant-microbe interactions and the adaptive mechanisms employed by root-associated bacteria during desiccation in M. flabellifolia. The transcriptional activity of …
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Multi-omics investigation of interactions between persistent bacteria and Salmonella in the inflamed gut
… is combined with the CBAJ-DB and used to recruit metatranscriptomic and metabolomic data from infected and uninfected CBA/J mice. We reveal expression and metabolites that implicate numerous commensal bacteria with the flow of sulfur in the inflamed intestine, making it available for host …
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