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Showing 1 to 7 of 7 for “"metaphase spreads"”.
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Characterizing Chromosomal Aberrations in Cells Deficient for Both ATM and MSH2
… genomic instability. To address this hypothesis, metaphase spreads were examined for chromosomal aberrations in B cells, which undergo genetically programmed DNA recombination and mutation. To bypass the lethality in <em>ATM<sup>-/-</sup>MSH2<sup>-/-</sup> </em>mice, a conditional knockout of ATM …
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THE EFFECTS OF AGE AND HETEROCHROMATIN ON FREQUENCIES OF ACQUIRED CHROMOSOMAL ANEUPLOIDY IN UNCULTURED HUMAN LEUKOCYTES
… overcoming problems inherent with the study of metaphase spreads for acquired aneuploidy assessment. Despite all the advantages of this technique, there are some limitations that could be misleading when scoring interphase autosomal aneuploidy. In this study we show that sex chromosomal …
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Analyse der Chromosomenverteilung in menschlichen Fibroblasten mittels 3D-Vielfarben Fluoreszenz in situ Hybridisierung
… corresponds to the procedure previously used for metaphase spreads, now adopted to 3-D studies of chromosome territory arrangements in the cell nucleus. The analysis of 30 G0-fibroblast nuclei and 30 prometaphase rosettes revealed a pronounced variability of chromosome territory neighbourhoods, as …
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Centromere identification and inactivation on a neo-Y chromosome fusion in threespine stickleback fish (Gasterosteus aculeatus)
… with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat (GacCEN) hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. To test whether epigenetic or genetic inactivation has occurred on the Japan Sea neo-Y …
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Mechanisms That Ensure the Proper Terminal Structure of Mammalian Telomeres
… between a fraction of leading-end telomeres on metaphase spreads. I next elucidated the mechanism by which the single-stranded telomere binding protein, POT1b, suppresses the accumulation of excessive telomeric overhangs in mouse cells. I found that POT1b inhibits 5' resection by Apollo at …
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A Systematic Evaluation of Methods to Separate X- and Y- Bearing Sperm
… was established using a number of standard metaphase spreads; from male and female subjects and an individual with Klinefelters syndrome. It was determined that dual colour FISH was a suitable technique for determining the percentage of X- or Y- bearing sperm within a sample. The semen …
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Incipient Speciation in Freshwater Fish Species from Two Isolated Watersheds
… was no difference between populations. Three metaphase spreads were obtained for each of the 22 Blacknose Dace for a total of 66 spreads examined. The karyotype consisted of 16 metacentric, 28 submetacentric, 2 subtelocentric, and 4 telocentric, yielding a diploid number of 2n=50 …