Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 122 for “"metaphase"”.
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The Impact Of Myeloperoxidase And Its Related Oxidants On Metaphase Ii Mouse Oocyte Quality
<p>Inflammatory reactions mediated by oxidative stress (OS) have been implicated in the deterioration of oocyte quality, which may lead to subfertility. Oxidative stress generated from enhancement of activated macrophages secondary to an inflammatory response are the major source of reactive oxygen …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
… an essential interplay between the two at the metaphase-arrested spindle.
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In Vitro Chromosomal Radiosensitivity and Cell Cycle Progression in Cancer Survivors
… DNA damage, which is exhibited at the subsequent metaphase as chromatid gaps and breaks. Radiation-induced arrest at the end of (32 is believed to allow time for adequate DNA repair before the onset of mitosis. Therefore, variation in the level of aberrations observed at metaphase is likely to be …
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Investigating the mitotic roles of Topoisomerase 2 Alpha and Topoisomerase 2 Beta-Binding Protein 1 in the maintenance of chromosome structure and genome stability
… thesis, I focus on the role of TOP2 in orderly metaphase congression and the maintenance of chromosome compaction during prometaphase-metaphase. These studies have been undertaken in human tumour-derived cell lines using both chemical inhibition and depletion (through siRNA and genetically …
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Regulation of chromosome attachment and dynamics by Saccharomyces cerevisiae kinetochores
… at mature chromosome-microtubule attachments in metaphase. Cin8p and Kip1p align metaphase chromosomes into the characteristic bilobed configuration that is analogous to the metaphase plate in higher eukaryotes. Kip3p regulates microtubule dynamics throughout the cell cycle and regulates poleward …
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Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila
… throughout mitosis and released at the metaphase-anaphase transition. In meiosis, sister-chromatid cohesion is released along the chromosome arms in the first meiotic division, but retained at the centromere until the second meiotic division. In this thesis, we have analyzed the …
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Cholesterol and egg activation
… in vivo, spontaneously activated; they escaped metaphase II (MII) arrest and progressed to pronuclear, metaphase III and anaphase/telophase III stages. Eggs from fertile, wild-type mice were activated when loaded in vitro with excess cholesterol using a cholesterol/methyl-[beta]-cyclodextrin …
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Role of Xenopus p21-activated kinase 2 (X-PAK2) in polar body formation during oocyte maturation
… these oocytes resume meiosis and progress to metaphase II, in which they await fertilization. The transition from prophase to metaphase II is termed oocyte maturation. During oocyte maturation, no mRNA synthesis is detectable. Instead, active protein translation from maternal mRNAs and …
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Role of Molecular Motors and Maps in Spindle Dynamics and Chromosome Segregation in the Fission Yeast Schizosaccharomyces Pombe
… mechanics affect chromosome segregation. The metaphase spindle is maintained at a constant length by an antagonistic force-balance model yet how the regulation of metaphase spindle length contribute to subsequent chromosome segregation remains unexplored. To test the force-balance model, I …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… to position each pair of chromosomes to the metaphase plate. Secondly, a surveillance system, known as the mitotic checkpoint, put the anaphase onset on hold until each pair of sister chromosomes are aligned at the metaphase plate and appropriately attached with microtubule plus ends by …
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Chromosome malorientation precedes nondisjunction during female meiosis I
… that chromosomes do in fact congress prior to metaphase I arrest in <em>Drosophila </em>requires a reexamination of the mechanisms behind nondisjunction. To do this, a series of <em>altered disjunction</em> (<em>ald</em>) mutant alleles generated by imprecise excision of a P element in the 5’ …
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Changes in proteins during the cell cycle of Physarum polycephalum
… concentration during early prophase, peaked in metaphase and decreased by a factor of five by one hour after metaphase. The identity of this protein is not known. Four forms of actin were purified and identified on two-dimensional gels. Their molecular weight was 43,000 with three isoelectric …
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The spindle checkpoint : Bubs, Mads, and chromosome-microtubule attachment in budding yeast
… functions such as invoking metaphase arrest following DNA damage.
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Mechanisms and Regulation of Organelle Transport in Xenopus Melanophores
… Xenopus egg extracts arrested in interphase or metaphase, we also demonstrated that actin-based melanosome motility is regulated in a cell cycle-dependent manner. In metaphase extracts, motility was inhibited as compared to interphase. This inhibition correlated with the dissociation of myosin-V …
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Analysis of the role of rab11-FIP3 phosphorylation during cytokinesis
… and temporal dynamics during mitosis. During metaphase and early anaphase, FIP3 is largely cytosolic, with some localised to endosomal membrane structures. During late anaphase, following furrow initiation, FIP3 localises to the centrosome. At late cytokinesis, FIP3 relocates to the cleavage …
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Characterizing Chromosomal Aberrations in Cells Deficient for Both ATM and MSH2
… genomic instability. To address this hypothesis, metaphase spreads were examined for chromosomal aberrations in B cells, which undergo genetically programmed DNA recombination and mutation. To bypass the lethality in <em>ATM<sup>-/-</sup>MSH2<sup>-/-</sup> </em>mice, a conditional knockout of ATM …
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Myosin Dynamics in Drosophila Neuroblasts Lead to Asymmetric Cytokinesis
… The cleavage furrow forms at the position of the metaphase plate so in asymmetric cytokinesis the spindle is shifted towards one pole. Unlike most systems, Drosophila neuroblasts have a centrally localized metaphase plate but divide asymmetrically. Drosophila neuroblasts divide asymmetrically due …
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Mitotic Dynamics of Normally and Mis-attached Chromosomes and Post-mitotic Behavior of Missegregated Chromosomes
… model of the forces and regulators controlling metaphase chromosome movement in the mammalian cell line derived from Potorous tridactylis kidney epithelial cells (PtK1) (Chapter 2). This model can explain key features of metaphase chromosome dynamics and related chromosome structural changes …
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Dissecting the Spatiotemporal Regulation of Yeast Kinetochore Interactions
… to kinetochores at basal levels from G1 through metaphase but accumulates abruptly at anaphase onset. How Cnn1 is recruited and which activities regulate its dynamic localization is unclear. We show that Cnn1 harbors two kinetochore-localization activities: a C-terminal histone-fold domain that …
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