Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 46 for “"metallothionein"”.
-
Regulation of tissue levels of metallothionein with emphasis on metallothionein degradation
The synthesis and degradation of metallothionein (MT) was studied in streptozotocin-induced diabetic rats and monolayer cultures of adult rat hepatocytes. Elevated levels of MT-I and MT-II were identified in the liver and kidney of untreated diabetic rats. The relative rates of hepatic and renal MT …
-
Induced Gene Expression In Bladder Cancer: Kindlin-2 And Metallothionein-1x
… for this type of cancer.</p> <p>Kindlin-2 and Metallothionein (MT) isoform-1X (MT-1X) are two genes that are the focus of this dissertation. Both genes were selected for further experimental analysis based on their common association with bladder cancer. Metallothionein isoforms 1 & 2 (MT I/II) …
-
Characterization of metallothionein expression in response to cadmium in the 267-B1, normal prostate cell line
The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were …
-
Identification, localization and metal-catalyzed induction of specific metallothionein isoforms expressed by the adult human lens
Purpose. Metallothioneins (MT s) are a large family of proteins involved in multiple protective pathways, including binding of toxic metals and oxidative stress. Previously we detected increased expression of MT IIA in age-related human cataractous lenses. As a first step towards defining the …
-
A Mass Spectrometry-Based Method For Quantification Of Human Metallothionein Isoforms And Usefulness In Biomarker Detection Of Cancers And Metal Toxicity
<p>Current methods for detecting metallothionein (MT) protein expression lack the specificity to distinguish between all twelve human isoforms. Each, however, can be distinguished by the masses of their acetylated, cysteine-rich, hydrophilic N-terminal tryptic peptides. These properties were …
-
Targeted Overexpression Of Metallothionein In Endothelial Cells Mitigates Pulmonary Alveolar Basement Membrane Thickening In Transgenic Diabetic Ove Mice: An Unbiased Tem Morphometric Analysis
… animal model that overexpresses the antioxidant metallothionein (MT) in endothelial cells. Unbiased TEM morphometry demonstrated that PABM widths in diabetic (OVE) mice were significantly (+20%) thickened. Remarkably, however, the bi-transgenic OVEJtmt progney exhibited statistically significant …
-
Characterization of Cu-rich aggregates in neurogenic niches of the rodent brain by X-ray fluorescence microscopy
… increase of Cu concentrations within aggregates. Metallothionein is a sulfur-rich protein known to be critical in Cu homeostasis in astrocytes cells. Metallothionein(1,2) knockout mice exhibit lower subventricular Cu concentrations owed in part to the fact that there are about one third as many …
-
Studies of a low molecular weight Zn-containing protein population of lens tissue
… proteins. Although the proteins isolated had metallothionein-like character in that they were of low molecular weight, contained Zn and had acidic pI values, the paucity of cysteine residues indicates that metallothionein is not a component of low molecular weight Zn-containing lens proteins.
-
Ecotoxicological and genetic effects of a mixture of heavy metals on selected aquatic macroinvertebrates
… exposed populations had significantly higher metallothionein concentrations. These findings suggest that long-term exposure to metals has resulted in tolerance, and that metallothionein may act as an underlying mechanism for this tolerance. To determine if there were underlying genetic …
-
Genetic variability and cadmium metabolism and toxicity
… uptake of Cd. Further, the zinc-binding protein metallothionein (MT) protects against toxicity of Cd in the kidney. Thus, the first study examined the association of single nucleotide polymorphisms (SNPs) in iron homeostasis genes with Cd concentrations in two groups of women, one from the …
-
Influence of genetic obesity on essential trace metal status and metabolism
… compared with lean mice. Constitutive levels of metallothionein were significantly different in several tissues of lean and obese mice. Together, these results demonstrate that chronic obesity alters tissue status of several micronutrients and zinc metabolism in ob/ob mice.
-
Interactions of cadmium, zinc, and phosphorus in marine Synechococcus : field uptake, physiological and proteomic studies
… a Zn-requiring alkaline phosphatase. A bacterial metallothionein, a Zn-associated protein, appears to be correlated with proteins present under low PO4 3- conditions. Together, these experiments suggest that the interactions of Cd and Zn can affect Synechococcus and play a role in the acquisition …
-
Reduction of Myocardial Stunning by in Situ Genetic Alteration of Myocytes
… injected a plasmid (PMTSOD) containing the sheep metallothionein promoter and cDNA for human Cu-Zn SOD into rat left ventricles in situ. A monoclonal anti-human CuZn SOD which does not cross-react with rat endogenous SOD was used to detect human SOD. Two to 14 days after injection of 400 μg …
-
Interactions of cadmium, zinc, and phosphorus in marine Synechococcus : field uptake, physiological and proteomic studies
… a Zn-requiring alkaline phosphatase. A bacterial metallothionein, a Zn-associated protein, appears to be correlated with proteins present under low PO4 conditions. Together, these experiments suggest that the interactions of Cd and Zn can affect Synechococcus and play a role in the acquisition of …
-
The Development of High Accuracy Methods for the Measurement of Metal Speciation
… The spike was shown to be stable. An oyster metallothionein (MT) was also produced using recombinant techniques. Methylmercury (MeHg) was determined in an International Measurement Evaluation Programme (IMEP) laboratory intercomparison tuna fish test sample, IMEP-20 tuna material. External …
-
Flux of cadmium through a laboratory food chain (media-algae-mussel) and its effects
… found to be very similar to those reported for metallothionein and had an approximate molecular weight of 10 600 daltons.
-
Copper exposure of freshwater mussels (Anodonta anatina): Some physiological effects
… and protein levels in various tissues, metallothionein induction, glutathione levels, activities of antioxidative enzymes and glutathione reductase, and on lipid peroxidation are examined. In the algal experiment, Parachlorella kessleri is grown at six 63Cu concentrations (0, 5.9, 11.7, …
-
Influence of molybdenum and sulfate supplementation and withdrawal of diets containing high copper broiler litter on tissue copper levels
… an apparent redistribution of Cu stores. Metallothionein (MT) increased 12-fold above control values in the litter-fed ewes (P<.05) and tended to decrease during the withdrawal period. Therefore, it appears that supplementing Mo and SO₄ to a diet containing high Cu broiler litter may …
-
The occurrence and toxicology of heavy metals in Chesapeake Bay waterfowl
… of copper and zinc, perhaps due to induction of metallothionein by cadmium. In combination with an imposed food restriction, cadmium ingestion appeared to alter some indices of energy metabolism, such as plasma concentrations of free fatty acids and triiodothyronine, at dietary cadmium levels far …
Page 1 of 3