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Showing 1 to 20 of 28 for “"metalloprotein"”.
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Metalloprotein Design
CcP has been used as a template for the purpose of producing a protein model of cytochrome P450 (cyt P450), and both CcP and swMb have been used as templates for engineering metal-binding sites characteristic of the active site in cytochrome c oxidase (CcO). Spectroscopic characterization of the …
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Using Unnatural Amino Acids and Nonnative Cofactors for Metalloprotein Design and Engineering
… entering the pocket will help design better metalloproteins.
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Engineering an oxygen storage metalloprotein into carbon dioxide and oxygen reduction metalloenzymes
Made available in DSpace on 2021-09-17T04:03:56Z (GMT). No. of bitstreams: 4 DWARAKNATH-DISSERTATION-2021.pdf: 5841120 bytes, checksum: ce2ff36f34d268a44ae248b23c61d93f (MD5) Dissertation Master Document_SD3.doc: 122368 bytes, checksum: 4309f4f0e2e18bdcfb871a2e77a38be9 (MD5) LICENSE.txt: 4217 …
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Metalloprotein engineering with unnatural amino acids: application in functional heme-copper oxidase and azurin
Metalloproteins, proteins with metal ion cofactors, are estimated to make up more than a third of total proteome. They involve in key biological processes such as photosynthesis, respiration, and nitrogen fixation. The important roles and potential applications of metalloprotein urges the need for …
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Metalloprotein Engineering Using Heme Protein Scaffolds to Investigate the Oxidation of Endogenous Aromatic Amino Acids and Exogenous Substrates
Protein engineering by rational design has been used to study heme proteins and artificial Mn-salen containing metalloenzymes using heme protein scaffolds. Heme proteins perform a wide variety of functions including the catalysis of numerous different reactions. Some of the structural features …
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Probing the Roles of Metal Binding Ligands in Cupredoxins: Incorporating Nonproteinogenic Amino Acids Into Azurin and Copper a Azurin
… role of the metal ligands and has shaped future metalloprotein engineering efforts.
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De Novo Heme Protein Design
… most natural way to explain this is that OR is a metalloprotein. We have found a consensus sequence ""HXXCE"" in the 4--5 loop of ORs, which not only binds strongly to Cu2+ and Zn2+, but also turns alpha helical after metal binding. Since the 4--5 loop is as hydrophobic as the fourth helix of OR, …
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Relationships between electron transport and redox center concentrations within molecular networks
… due to packing. Hard-sphere $D\sb E$ values for metalloprotein kinetics and a redox molecular diameter of 13 A (corresponding to Ru(bpy)$\sb3\sp{2+}$) are 1.9 $\times$ 10$\sp{-10}$ cm$\sp2$s$\sp{-1}$ and 5.0 $\times$ 10$\sp{-9}$ at 0.10 $M$ and 1.07 $M$, respectively.
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Overcoming barriers in chemistry : a birds eye view of electrostatic fluctuations in fundamental proteins and streamlining inclusivity in the classroom.
… the ability of a Rieske Iron-Sulfur (ISP) metalloprotein to accept an electron with varying pH (5.5 – 10.6) and the effect this electron transfer has on the overall net charge. Additionally, the trends in the Rieske ISP's Z and ∆ZET are explored as well as the ∆ZET and protonation free …
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Multinuclear NMR Studies of Hemoproteins and Their Model Compounds
… The first iron-57 NMR spectra of a metalloprotein, carbonmonoxymyoglobin, has been obtained, yielding the isotropic chemical shift, the anisotropy of the chemical shielding tensor and the rotational correlation time of the protein. The oxygen-17 NMR signals from CO ligands bound to …
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High relaxivity biomolecule based contrast agents engineered for molecular functional magnetic resonance imaging
… protein design to engineer a high relaxivity metalloprotein by tuning phenylalanine hydroxylase to bind gadolinium at high affinity. Mutations were found that increased the protein affinity by two orders of magnitude and enhanced relaxivity. The results of this thesis advance approaches for …
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Electrostatic control of Cu, Zn superoxide dismutase aggregation in Amyotrophic Lateral Sclerosis : from lysine modification to interaction with lipid membranes.
… Zn superoxide dismutase (SOD1) is a ubiquitous metalloprotein, which is responsible for protecting living cells from oxidative stress via disproportionation of superoxide ions. Misfolding and subsequent aggregation of SOD1 is casually linked to familial and sporadic cases of amyotrophic lateral …
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Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… A. Finally, ICP analysis revealed LctM is a metalloprotein containing one equivalent of zinc. Mutation of the zinc ligands C781 and C836 to alanine in LctM did not affect dehydration activity but did impair cyclization activity of the protein. These results implicate the C-terminal domain of …
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Thermodynamic Investigations of Metalloproteins: Metal as Probe and Protein as Probe
<p>In this dissertation several metalloproteins, both metal transport proteins and the classic metalloprotein hemoglobin, are investigated using a variety of biophysical and electrochemical techniques. In each case, thermodynamic measurements provide insight into the role and mode of action of the …
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Understanding the Speciation of Ruthenium Arene Complexes as Precursors to Unnatural Enzyme Cofactors
… introduced into a protein scaffold or a natural metalloprotein being evolved to perform catalytic reactivity. Taking inspiration from naturally occurring metalloproteins, this research showed that it was possible to form ArMs via a ligand exchange process between a ruthenium arene precursor …
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Rational design of functional heme copper oxidases in myoglobin
… most cellular pathways that have at least one metalloprotein performing one or more steps. Many, if not all, of the most important and complex processes that occur in living systems are performed by a metalloprotein. These processes include photosynthesis, cellular respiration, and nucleic acid …
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Isolating, characterizing, and engineering novel Cu-proteins and peroxidases
Metalloproteins are a fascinating class of proteins that function at the heart of several important biological processes including photosynthesis, respiration, and nitrogen fixation. It is even more amazing, considering that nature uses a small set of tertiary structures and metal centers to …
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Nitric oxide reactions of bio-Inspired zinc and cobalt complexes
… discussed. Bioinspired small molecule models for metalloprotein active sites are introduced, and the ability of ligands to control the steric and electronic properties of coordinated metal centers is demonstrated. The bioinorganic chemistry of NO and its targets is described in the context of the …
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Molecular basis oh herpes simplex virus entry into the cell and retargeting of the viral tropism for the design of oncolytic herpesviruses
… of gH in complex with gL under the control of a metalloprotein inducible promoter and purified the heterodimer by means of ONE-STrEP-tag system by IBA. With respect to biological function, the purified heterodimer is capable: • of reacting to antibodies that recognize conformation dependent …
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The Effect of Three Fescue Types and Lakota Prairie Grass on Copper Status, Dry Matter Intake, and Alkaloid Appearance of Beef Steers
… dismutase (SOD) is a Cu-based antioxidant metalloprotein that acts as a defense against oxidative stress by the scavenging of O₂⁻. <i>Neotyphodium</i>-infected tall fescue is typically lower in Cu which could potentially increase oxidative stress of animals grazing this forage. Therefore …
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