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Showing 1 to 20 of 58 for “"metalloprotease"”.
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Function of the ATP-dependent Metalloprotease FtsH during sporulation in Bacillus subtilis
The analysis of the function of the ftsH gene of Bacillus subtilis started about ten years ago. It was shown at that time that an ftsH knockout was viable, but exhibited a pleiotropic phenotype. Cells are sensitive to salt and heat shock, exhibit filamentous growth, are difficult to be transformed …
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Interaction Between the Metalloprotease ADAMTS-13 and the Proteins of the Alternative Pathway of the Complement System
Von Willebrand factor (VWF), a multimeric protein that has a central role in hemostasis, has been shown to interact with complement components. However results are contrasting and inconclusive. By studying 20 patients with congenital thrombotic thrombocytopenic purpura (cTTP) who can not cleave VWF …
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Localization of a Microsporidia ADAM (A Disintegrin and Metalloprotease Domain) Protein and Identification of Potential Binding Partners.
… to members of the ADAM (a disintegrin and metalloprotease) family of type I transmembrane glycoproteins. The microsporidia ADAM (MADAM) protein is of interest because ADAMs are known to be involved in a variety of biological processes including cell adhesion, proteolysis, cell fusion, and …
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HUMAN CLCA2 IS A P53-DEPENDENT ZINC METALLOPROTEASE AND ITS INTERACTION WITH EVA1 MAINTAINS DIFFERENTIATION OF HUMAN MAMMARY EPITHELIAL CELLS
… expressing wild type CLCA2, suggesting that the metalloprotease activity contributes to the anti-proliferative effect of CLCA2. Physiologically, CLCA2 plays an essential role in epithelial differentiation. It is induced during epithelial differentiation in immortalized human mammary epithelial …
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EFFECT OF HISTIDINE TAG ON THE ENZYMATIC ACTIVITY OF PSEUDOLYSIN AND ITS INTERACTION WITH THE STREPTOMYCES METALLOPROTEASE INHIBITOR (SMPI) PROTEIN
… PLN belongs to the family of zinc-dependent metalloproteases. It is naturally inhibited by a smaller protein called streptomyces metalloprotease inhibitor (SMPI), secreted by <em>Streptomyces nigrescens</em>. Previous and current studies demonstrate that the interaction between PLN and SMPI …
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The Role of the Propeptide and its Residues in Activation and Secretion of Elastase, an M4 Metalloprotease Secreted by Pseudomonas aeruginosa
Pseudomonas aeruginosa secretes several proteases associated with pathogenesis, but the most abundant and active is elastase (M4 metalloendopeptidase). Elastase (lasB), is first synthesized as a preproenzyme, with a signal peptide, an 18-kDa N-terminal propeptide, and a 33-kDa mature domain. The …
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Evaluating The Impact of Post-Translational Modifications By The Secreted Zinc Metalloprotease, Gele, On The Major Autolysin of E. Faecalis, Atla, and A Stress-Induced Protein, Salb
… gelatinase (GelE). GelE is a secreted zinc metalloprotease known to impact various cellular functions by post- translational modification of protein substrates. The overall objective of this work was to understand how GelE cleavage of secreted proteins, specifically AtlA and SalB, changes …
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Asthma: Cytokine-Induced Over-Expression of Matrix Metalloproteases Compromises Airway Epithelium Tight Junctions and a Clinical Case Study
… activity that up regulates expression of matrix metalloprotease 9. MMP9 encourages detachment-induced cell death - anoikis - which results in a structural change compromising the airway epithelium's ability to act as a primary immune defense mechanism. The chronic characterization of asthma …
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Investigating Invadolysin's activity: discerning mechanism and cellular roles
Invadolysin is a novel metalloprotease, which is conserved amongst metazoans and was first identified in the Heck laboratory. Proteases play a variety of roles in normal physiology. Invadolysin is essential for life in Drosophila. Invadolysin has been shown to be essential for cell division and …
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The structure of testis angiotensin-converting enzyme (tACE-g13) in complex with the inhibitor RXPA380
Angiotensin-converting enzyme (ACE), a zinc metalloprotease, is a key regulator of the mammalian renin-angiotensin system (RAS) Primarily, ACF is a dipeptidl peptidase which cleaves angiotensin I to produce angiotensin II, a potent vasoconstrictor. By the same enzymatic mechanism, ACE also …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… Neurotoxin type A Light Chain (BoNTA/LC) metalloprotease. Supplementary studies include assays performed involving the Bacillus anthracis Lethal factor metalloprotease. In the work described we have prepared active recombinant protease substrates in our laboratory, capable of binding with …
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Identification of seminal proteins related to fertility of bulls
… (TIMP-2). TIMP-2 regulates matrix metalloprotease activity and therefore, potentially plays a key role in the structural makeup of the extracellular matrix. These findings suggest that regulation of enzymatic activity in seminal fluid is in large part a function of heparin binding …
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Nutrient availability modulating physiology and pathogenicity of <i>Legionella pneumophila</i>
… of the important pathogenicity factor, the zinc metalloprotease, was regulated by iron availability. Common iron acquisition mechanisms, such as siderophores and transferrin receptors, were not elaborated by iron-limited cells. However, human transferrin was identified as a potential iron source …
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Interrogating regulated intramembrane proteolysis of IGF-1R, and its role in cell migration
… shedding of IGF-1R is likely catalysed in a metalloprotease dependent fashion, consistent with other receptor tyrosine kinases. We also confirmed that the CTF can undergo nuclear translocation in a clathrin-mediated endocytosis dependent manner, consistent with the translocation of the …
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Auswirkungen der maternalen HIV-1-Infektion auf die Genexpression endothelialer Vorläuferzellen und differenzierter Endothelzellen nicht-infizierter Kinder
… Mütter die Expression von Matrix-Metalloprotease-1 (MMP-1) unter der Nachweisgrenze liegt oder signifikant reduziert ist. Dies konnte auf RNA- und Proteinebene sowie mittels Gelatine-Zymografie bestätigt werden.
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Quantitative studies of EGFR autocrine induced cell signaling and migration
… precursors modulated by the addition of the metalloprotease inhibitor batimastat. We found that ERK activation increased with increasing ligand release rates despite concomitant EGF receptor downregulation.
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Adamts9 is critical for the development of primary ovarian follicles
Adamts9 (a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 9) is an extracellular matrix metalloprotease that is highly evolutionarily conserved and critical for development in vertebrates. Knockouts of ADAMTS9 in mice and AdamTS-A in Drosophila melanogaster are embryonic …
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Chemical biology of matrix metalloproteases
Matrix Metalloproteases (MMP) inhibition is currently brought to the focus of medicinal chemistry research due to their essential roles in many human diseases. To elucidate the functions of each individual MMP, it is imperative to synthesise small molecule/probes which can selectively target a …
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Insulin-Like Growth Factor Binding Protein-6: Posttranslational modifications and sorting in polarized MDCK cells
… gespalten wird - eine Serin- und eine Disintegrinmetalloprotease (ADAM). Polypeptide in partiell gereinigten, Metalloprotease-haltigen Fraktionen kreuzreagierten mit Antikörpern gegen konservierte Domänen von ADAM 12 S in Western immunoblots. Diese Daten lassen vermuten, dass die IGFBP-6 …
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Determination of ADAMTS13 Susceptibility in Type IIA von Willebrand Disease
… the coagulation cascade. vWF is cleaved by the metalloprotease ADAMTS13, regulating the multimers size, which hinders vWF’s ability to function properly. The three main types of vWD —Type I, II, and III— are not well defined, and as a result are all similarly treated with plasma-derived vWF …
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