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Showing 1 to 20 of 36 for “"metalloenzyme"”.
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Development Towards an Ethylene Sensing Artificial Metalloenzyme
… a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium probe. Efforts towards the expression and purification of the protein scaffold are also here reported.</p> …
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
<p>Nitrile hydratases are metalloenzymes that catalyze the hydration of nitriles to their corresponding amides in a specific manner at ambient pressures and temperatures at neutral pH. Traditional industrial methods require high temperature and pressure, extreme pH, and heavy metals. NHases are …
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Design, engineering & characterization of a PQQ containing artificial metalloenzyme
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-20 without embargo terms
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Structural studies of metalloenzyme complexes in acetogenic carbon fixation
… carbon from CO₂. This process requires several metalloenzymes that employ transition metals such as iron, nickel, and cobalt towards the production of acetyl-CoA, the final product. In one stage of the pathway, the cobalt-containing B₁₂ cofactor harbored by the corrinoid iron-sulfur protein …
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Recombinant Human Betaine -Homocysteine S -Methyltransferase: Discovery as a Zinc Metalloenzyme, and Regulation of Activity by Redox Status
The lack of activity in the absence of reducing agent is not due to loss of Zn at the catalytic site. When reducing agent-free preparations of 5Cys/Ala are incubated with the Zn chelator, PAR, and the absorbence of the Zn-PAR chelate is monitored, there is no change in absorbence unless …
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Characterization of the Xerophyta humilis desiccation induced-1 (Xhdsi-1voc) gene : a member of the Vicinal Oxygen Chelate (VOC) metalloenzyme superfamily upregulated in X. humilis (BAK) DUR and SCHINZ during desiccation
The resurrection plant, Xerophyta humilis is used as a model system to identify and characterise genes which play an important role in conferring desiccation tolerance in plants. In this study, the expression of a novel gene named desiccation induced-1 (dsi-1VOC) during desiccation in X. humilis …
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Enantioselective epoxidation catalyzed by manganese-substituted human carbonic anhydrase
<p>Artificial metalloenzymes are an important class of hybrid catalysts for enantioselective, regioselective and chemoselective organic transformations. Despite several limitations associated with this type of hybrid catalysts system and their limited development relative to small molecule …
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Isolation and Characterization of Reactive Heme -Oxygen Intermediates in Cytochrome P450 Catalysis
… utilization of related intermediates in other metalloenzyme systems. In order to assess the commonality of these intermediates, the ferric-peroxo species have also been generated in a number of other heme enzyme systems in order to assess the role of proximal ligand and distal pocket in the …
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Allosterische Kontrolle von Katalyse- und Rezeptoreigenschaften mehrkerniger Metallkomplexe
… (L1 – L3) als Modellsysteme für allosterische Metalloenzyme untersucht. Die Metallkomplexe sind aus einer allosterischen Untereinheit und einer katalytischen Untereinheit aufgebaut. Ein mononuklearer Co(III)-Komplex des Liganden L3 konnte isoliert und kristallographisch charakterisiert werden. …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
Metalloenzymes utilize metallocofactors, ranging from single metal ions to complicated metallic clusters, to catalyze a wide range of challenging chemical reactions that are critical for life. Incorporation of these metallocofactors often relies on proteins known as metallochaperones that …
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Exploring SOD1 electron transfer, heterodimerization, and hetero-amyloid formation.
… Zn Superoxide dismutase (SOD1) is an essential metalloenzyme that regulates superoxide radicals. Because SOD1 is a long-lived protein, it contains an intrinsic molecular clock, deamidation, that accumulates over time. Misfolding and aggregation of SOD1 have been linked to neurodegenerative …
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Engineering heme-copper and multi-copper oxidases for efficient oxygen reduction catalysis
… to that of native enzymes in a designed metalloenzyme offers deeper insight into the roles of tunable processes such as ET in oxidase activity and enzymatic function and may extend into applications such as more efficient oxygen reduction reaction catalysts for biofuel cells. The other …
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Investigating the Biochemical and Catalytic Properties of Nitrile Hydratases
… hydratases (NHase, E.C. 4.2.1.84). NHase is a metalloenzyme that efficiently converts nitriles to amides at neutral pH and ambient temperatures, thus reducing production of unwanted by-products and toxic wastes. NHase contains either a non-heme Fe<super>3+</super> or a non-corrin Co …
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Metalloprotein Engineering Using Heme Protein Scaffolds to Investigate the Oxidation of Endogenous Aromatic Amino Acids and Exogenous Substrates
… heme proteins and artificial Mn-salen containing metalloenzymes using heme protein scaffolds. Heme proteins perform a wide variety of functions including the catalysis of numerous different reactions. Some of the structural features involved in conferring these functional properties have been …
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Structural enzymology of soluble methane monooxygenase protein-protein interactions.
… methane monooxygenase (sMMO) is a multicomponent metalloenzyme that activates molecular oxygen, breaks the 105 kcal/mol C-H bond of CH4, and inserts one atom of O to create methanol at ambient temperature and pressure. This feat of catalytic prowess requires all three protein components of sMMO …
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Substrate interactions during the anaerobic biodegradation of 1,1,1-trichloroethane
… by the intracellular concentration of a reduced metalloenzyme (the dehalogenase). The concentration of the reduced dehalogenase is controlled by the external concentrations of the electron donor and acceptor. Although Monod kinetics adequately described the relationship between TCA concentration …
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Allosterische Metallkomplex-Katalysatoren : Synthesen, Strukturen und reaktionskinetische Untersuchungen
… als Modellsysteme für allosterische Metalloenzyme untersucht. Die Katalysatoren sind durch eine dinukleare, katalytische Untereinheit und eine allosterische Untereinheit mit strukturellem Metallion (MS=M(II)) charakterisiert. Von den isolierten Komplexen, darunter 4 mononukleare …
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Studies of the nature of Burkholderia cepacia in cystic fibrosis
… enzyme was assigned PCM-I (Pseudomonas cepacia metalloenzyme I).
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The Effects of Zinc on Skeletal Alkaline Phosphatase Activity and Skeletal Tissues
… high levels in osteoblasts, is a zinc-containing metalloenzyme and is important in bone formation. Preliminary studies showed that low levels of zinc could increase ALP specific activity in human osteosarcoma, SaOS-2 cells. Current studies were designed to characterize this zinc effect, test …
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Rational Design of Catalytic Metal Complexes for Potential Applications in Anticancer Chemotherapy
… in a novel fashion as small-molecule artificial metalloenzyme-like drug candidates. Specifically, we were interested in disrupting the strict redox homeostasis found inside the intracellular environment of cancer cells. The research described herein details the development process of the …
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