Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"metallochaperone"”.
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An old cofactor in a new light : how nature handles and repurposes adenosylcobalamin
… of AdoCbl require specialized machinery such as metallochaperones. In this thesis, I illustrate these different facets of the biochemistry of AdoCbl. Using X-ray crystallography, I determined structures of IcmF, a fusion protein between AdoCbl-dependent isobutyryl-coenzyme A mutase and a …
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Investigating cofactor transfer for a B₁₂-dependent enzyme
… MCM relies on two other proteins, a G-protein metallochaperone called methylmalonic aciduria type A protein (MMAA) and a protein called adenosyltransferase (ATR), to load and off-load cofactor. Mutations or deletions of the gene for MCM, or in any of the genes corresponding to accessory …
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Investigation of Escherichia coli [NiFe]-Hydrogenase Maturation
… protein, a process that is accomplished by the metallochaperone proteins HypA, HypB, and SlyD. This delivery process is supported by proteins that import, export, regulate, and store nickel to ensure a sufficient supply while also mitigating the innate toxicity of this transition metal. In this …
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METAL TRAFFICKING DURING LIMITATION AND EXCESS IN BACILLUS SUBTILIS
… expression of the zinc importer ZnuABC and the metallochaperone ZagA. It is unknown how ZagA acquires zinc, where the zinc is chaperoned, or how metal transfer takes place. Here we discuss the idea that ZagA may acquire zinc from ZnuABC or zinc-containing ribosomal proteins. Potential clients of …
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Mechanistic studies of nitrile hydratases and their activator proteins
… Both the Fe-type and Co-type NHase require a metallochaperone or ‘activator’ protein for maturation to the fully functional state. Through spectroscopy, crystallography, and computational methods, this research elucidates key mechanistic features and provides novel insights into the NHase …
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Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
… of signal attenuation by interaction with the metallochaperone CusF is also discussed. These data help construct a model for signal transduction in the Cus system and help characterize, for the first time, a metal-responsive sensor histidine kinase in E. coli.
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Understanding The Molecular Mechanism Of Manganese Oxidation In Leptothrix Discophora Ss1
… putative cytochrome c next to a putative copper metallochaperone (Sco1/SenC/PrrC) involved in the biogenesis of cytochrome oxidase. Further analysis of the nonmanganese oxidizing mutants identified through transposon mutagenesis coupled with the draft genome sequence of Leptothrix discophora SS1 …
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Characterization Of Arsd: An Arsenic Chaperone For The Arsab As(iii)-Translocating Atpase
… dimerization is not strictly required for ArsD metallochaperone function.</p> <p> ArsA and ArsD crystal structure have been solved individually. But little is known about ArsA-ArsD interaction interface. Yeast two-hybrid and reverse yeast two-hybrid are combined to select for totally 14 ArsD …
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Characterization Of Initial Iron Binding Location And The Structure/iron Binding Site On S.cerevisiae Isu And On D.melanogaster Frataxin
<p>Iron-induced free radical damage has been implicated in the pathology of diseases of iron overload such as Friedreich's Ataxia, a genetic disorder characterized by an accumulation of iron in actively metabolizing tissues ultimately leading to cardio- and neuro- degeneration and cell death. It is …