Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"metal-homeostasis"”.
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The Role of Compartmentalized Metabolism in Cellular Metal Homeostasis
… need for iron, also play a key role in iron homeostasis. Mitochondria are the sites of iron assimilation whereas lysosomes, with their v-ATPase-generated acidic lumens, are responsible for iron uptake and rely. In the first part of this work, I focused on lysosomes. Unbiased genetic screens …
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Cellular Copper Import Mechanisms via Secreted Cuproproteins: Exploring Metal Homeostasis in Pseudogymnoascus destructans
This thesis investigates the metal stress response of the fungal pathogen Pseudogymnoascus destructans (Pd), which causes White-Nose Syndrome in bats. Special emphasis is placed on understanding how cellular metal status effects Bim1-like Proteins (BLPs) and high-affinity copper transporters (CTR …
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Oxidative stress and metal homeostasis at the air-lung interface in Chronic Obstructive Pulmonary Disease
… the lavage samples from chapter 2. Pro-oxidant metal (Fe and Cu) concentrations were determined in the lavage samples from each of the groups used in chapter 2, by inductively coupled plasma mass spectrometry and a novel assay was developed based on metal catalysed oxidation of ascorbate, to …
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The evolution and regulation of DNA-binding by the nickel-dependent transcription factor NikR
Transition metal homeostasis is critical for all cells to balance cellular metal requirements with metal availability. One common homeostatic mechanism in bacteria is metal-dependent transcriptional regulation. The Ni<super>2+</super>-dependent transcription factor NikR is a member of the …
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Describing the Cell Biology of Saprolegnia parasitica: Role of Metals and Respiration
… how S. parasitica responds to fluctuations in metal availability at the physiological and mitochondrial levels. This study investigates the impact of copper and iron availability on Saprolegnia parasitica growth, metal uptake, and respiratory function. We cultured S. parasitica in chemically …
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Biosynthetic and Functional Studies of Bacillithiol in Gram-positive Bacteria
… post-translational modification, and metal homeostasis. Previous studies have identified glutathione (GSH) as the dominant LMW thiol in eukaryotic cells as well as Gram-negative bacteria and mycothiol (MSH) as the dominant LMW thiol in Actinobacteria. Functional studies on those thiols …
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Investigation of microbial responses to transition metal sequestration by the innate immune protein calprotectin
… to microbial infection, the human host deploys metal-sequestering host-defense proteins to limit nutrient availability and thereby inhibit microbial growth and virulence. Calprotectin (CP) is an abundant antimicrobial protein that is released from neutrophils and epithelial cells at sites of …
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Functional Characterization of Metallothioneins in Arabidopsis and Barley
<p>Metalothioneins (MTs) are metal binding proteins that can bind metals such as Cu, Zn, and Cd but the functions of MTs in plants are largely unknown. To understand the function of MTs in Arabidopsis, T-DNA insertion mutants lacking 4 MT genes including <em>MT1a, MT2a, MT2b</em> and <em>MT3</em> …
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Role of Extracytoplasmic Function Sigma Factors in Porphyromonas gingivalis
… involved in oxidative stress protection and metal homeostasis in SigG deficient mutant designated as V3085. Our survival studies showed that both SigH and SigG are essential for P. gingivalis to grow in host cells. Collectively our studies demonstrate that SigH is a positive regulator of gene …
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Functional Genomics and physiology of growth initiation in Salmonella
… included those encoding systems involved with metal ion uptake, stress resistance, phosphate uptake, ribosome synthesis and cellular metabolism. Genes in the OxyR regulon were induced earlier in cold-stored cells, a response coupled with a delay in the expression of Fe2+ acquisition genes, and …
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Copper as an Antibacterial Agent and Disruptor of Protein Stability
… activity and are believed to disrupt metal homeostasis within the microbes they kill. Molecules called ionophores shuttle Cu into the cell to poison it. However, the antibacterial modes of action behind Cu and small molecule ionophores are not well understood. In this work, we employ a …
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Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
Transition metals such as copper, zinc and nickel are required in many enzymatic processes that require redox changes. When transition metal concentration exceeds a certain threshold, their redox and metal binding properties make these elements extremely toxic. Bacteria regulate the cellular …
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Effects of copper on calcium metabolism and detoxification mechanisms in freshwater bivalve species of Anodonta
Copper (Cu) is one of the metals contaminating European fresh water ecosystems. Filter feeding bivalves have high bioaccumulation potential for transition metals as Cu. While copper is an essential micronutrient for living organisms, it causes serious metabolic and physiological impairments when in …
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Investigation of Escherichia coli [NiFe]-Hydrogenase Maturation
… the reversible oxidation of hydrogen gas at a bimetallic active site and are important enzymes in bacteria and archaea for anaerobic growth and pathogenesis. The maturation of [NiFe]-hydrogenase requires at least seven dedicated accessory proteins to assemble and insert the components of the …
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Identification and characterization of a novel class of hydrogen peroxide targets in escherichia coli
… (Rpe), which requires a solvent-exposed divalent metal for activity, was found to be sensitive to H2O2 both in vivo and in vitro. Although Zn, Mn, Fe, and Co were found to activate Rpe to varying degrees, only Rpe loaded with Fe was sensitive to H2O2, which suggests that Fe is the metal that …
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Genetic and Biochemical Studies of YqjH and YqjI: Cross-talk between Iron Homeostasis and Nickel Homeostasis in Escherichia Coli
<p>Metals are important for maintaining proper cellular functions. Cells evolved metal</p> <p>homeostasis systems to control metal acquisition and utilization in response to environmental changes in order to fulfill the cellular metal demand under different situations. Extensive studies have been …
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Proteomic and genomic characterization of the influence of copper on Legionella pneumophila and the drinking water microbiome
… copper exposure via the induction of redox and metal homeostasis proteins, while concomitantly inducing motility and pathogenesis related proteins, suggestive that copper induces a search for a host protozoan strain for protection. In 2014 and 2015, Flint, Michigan experienced the largest per …
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Copper at the Interface of Chemistry and Biology: New Insights into hCtr1 Function and the Role of Histidine in Human Cellular Copper Acquisition
<p>Mechanisms of copper homeostasis are of great interest partly due to their connection to debilitating genetic and neurological disorders. The family of high-affinity copper transporters (Ctr) is responsible for extracellular copper acquisition and internalization in yeast, plants, and mammals, …