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Showing 1 to 20 of 38 for “"metal affinity"”.
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Isolation of immunoglobulins from cheese whey and milk using ultrafiltration and immobilized metal affinity chromatography
… concentrated whey was applied to an immobilized metal affinity chromatography (IMAC) column to isolate IgG. With a 50 ml IMAC column loaded with 17.3 ml of 50 mM CuCl₂ to get 2/3 Cu saturation, 1240 mg of IgG could be isolated with 56% purity. About 152 mg of lactoferrin and 90 mg of …
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Studies on human protoporphyrinogen oxidase
… their purification to homogeneity by single step metal affinity chromatography.
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Development of micro analytical devices
… of biological reagents using immobilised metal affinity chromatography at micro-litre scale. Chapter 1 provides a general overview and background to the field of process analytics, microreactors and theory related to the mass transfer inside the electrochemical microfluidic devices and …
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Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration
… designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… pastoris to produce VGLUT1 protein. Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… pastoris to produce VGLUT1 protein. Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were …
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Characterization of Residual Mineral Content of Bauxite Stockpiles in Saline County, Arkansas: An XRD Analysis Approach
… zirconium, and titanium, suggesting trace metal affinity. A strong inverse relationship between gibbsite and kaolinite (r = -0.96) indicates differing formation pathways. Anatase and rutile show weak correlation (r = 0.16), implying variable titanium mobility. These findings highlight …
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The expression and purification of the regulatory domain of PKG1 alpha
… contaminations. With use of immobilized-metal affinity, ion exchange, and size exclusion chromatography, I successfully purified the regulatory domain of type 1 alpha kinase yielding 490 µL of the protein at a concentration of 2.2 mg/ml that is completely free of cAMP for the first time. …
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A biophysical and biochemical approach to understanding the interplay between Quaternary structure and function of human calprotectin
… One component of the response involves metal-sequestering proteins that starve pathogens of essential metal nutrients. Humans release calprotectin (CP), a heterooligomer of S100A8 and S100A9, from neutrophils and epithelial cells to prevent microbes from accessing manganese, iron, …
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Photovoltaic devices using photosynthetic protein complexes
… reaction centers oriented via an engineered metal-affinity polyhistidine tag. Various analytical and spectroscopic techniques have been used to examine solution spectrum and solid state device characteristics. Reasonable efficiencies have been obtained which demonstrates applicability of such …
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Characterization of an altered MoFe protein from a nifV- strain from Azotobacter vinelandii
… molybdenum iron protein. FeMo co consists of a metal sulfur core composed of an FeS cluster bridged by three inorganic sulfides to a MoFeS cluster. An organic acid, homocitrate, is coordinated to the Mo atom through its 2-carboxy and 2-hydroxy groups. Homocitrate is formed by the condensation of …
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Metal-protein interactions for proteomics applications
… protein depletion and sample fractionation. Metal ions play critical roles in most protein enzymes and their interactions with proteins have been well documented, but their applications in proteomics are underdeveloped. We have been developing and applying metal-protein interaction based …
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BIOSENSING OF MATRIX METALLOPROTEINASE ACTIVITY WITH CD-FREE QUANTUM DOTS
… has gone into developing QDs without toxic heavy metals, generally Cd, that would still yield comparable performance in terms of their optical properties. Nonetheless, even in the case of Cd-free QDs, toxicity should be evaluated on a case by case basis, as other properties such as size, coating, …
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
… the purification of the Pik1-Frq1 complex on a metal affinity resin, with the ultimate goal being to test the Sec14 presentation mechanism by measuring the kinase activity of Pik1 from purified fractions in the presence and absence of Sec14.
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Heavy metal adsorption on iron oxide and iron oxide-coated silica : macroscopic, spectroscopic, and modeling studies
To accurately model metal mobility and bioavailability in soils and sediments, systematic sorption studies are needed using representative and well-characterized minerals. Two important surfaces are iron oxide and silica, which are ubiquitous and associated with one another in the environment …
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Leishmania CRK3:CYC6 cyclin-dependent kinase as a drug target
… and co-purified from Escherichia coli via metal affinity and gel filtration chromatography to obtain a 1:1 ratio of CRK3:CYC6 proteins, which formed a stable protein kinase complex. Using histone H1 as a substrate, active CRK3:CYC6 was used to develop a radiometric assay suitable for low to …
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Design and synthesis of biocompatible fluorescent semi-conductor nanocrystals for in-vivo and in-vitro imaging/sensing applications
… and attachment of proteins to enable high-affinity cell labeling and single particle tracking. In addition, QDs solubilized with these ligands could be derivatized via metal-affinity driven conjugation chemistry with polyhistidine-tagged proteins, which facilitated the purification of …
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Proteomic Analysis of the Flavonoid Biosynthetic Machinery in Arabidopsis Thaliana
… mass spectrometry, with well-established affinity chromatography methods to further characterize the organization and composition of the Arabidopsis flavonoid enzyme complex. One of the key flavonoid enzymes, chalcone isomerase (CHI), was used in these experiments to detect interacting …
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Characterization of protein microstructure by various chromatographic techniques
… exchange chromatography (IEC) and 2) immobilized metal affinity chromatography (IMAC), were used to characterize the above-mentioned protein structure parameters. The model protein chosen for our work is T4 lysozyme. The protein consists of 164 amino acids with molecular weight ~ 18 kD. SYBYL 7.1 …
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Mechanism of tocopherol transfer by human α-tocopherol transfer protein (α-hTTP)
… that co-purified with 6-His-a-hTTP from the metal affinity column as the source of the problem. In order to completely avoid OmpF contamination, a GST -a-hTTP fusion protein was purified from a glutathione agarose column followed by an on-column thrombin digestion to remove the GST tag. We …
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