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Showing 1 to 13 of 13 for “"metagenomic library"”.
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Natural Products From Functional Screening of Soil Metagenomic Libraries
… a strategy that eludes 99% of the microbiome. Metagenomics offers a route to study uncultured bacteria by extracting DNA present in environmental samples and cloning this eDNA into a vector to make metagenomic libraries. Libraries can then be screened in various ways to find natural products …
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Functional metagenomic analysis of carbohydrate degrading enzymes from the human gut microbiota
… based on functional screening of genomic and metagenomic libraries using Escherichia coli and Lactococcus lactis as heterologous expression hosts, was used to isolate novel genes encoding glycoside hydrolase (GH) enzymes. The study identified several active GH enzymes involved in the breakdown …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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Pure culture and metagenomic approaches to investigate cellulose and xylan degradation
… from novel actinomycete isolates and the use of metagenomics to uncover previously unidentified cellulase genes was undertaken. Actinomycetes were cultivated from soil samples and the isolate with the best cellulase and xylanase activity was subjected to strain improvement through protoplast …
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Physiological effects of heterologous expression of proteorhodopsin photosystems
… PRPS previously identified from a large insert metagenomic library (Martinez et al., 2007). Incorporation of the PRPS into a strain of Pseudomonas putida resulted in a light-dependent increase in viable cell yield of cultures grown in low carbon media. The light-dependent effect demonstrates a …
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Natural Product Biosynthesis In Uncultured Bacteria
… due to cloning and expression limitations of metagenomic library host strains. Second, the lack of robust and scalable gene cluster assembly methods precludes the functional characterization of a large number of natural product biosynthetic gene clusters from cosmid-based eDNA libraries. …
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Identification of Quorum Sensing Genes in Hypersaline Microbial Mats
… natural ecosystems. This study used functional metagenomic techniques to identify quorum sensing genes in microbial mat communities obtained from</p> <p>Salt Pond on San Salvador Island, The Bahamas. We first extracted high molecular weight metagenomic DNA (> 48 kb) from the microbial mats and …
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Genetic analysis of bacteriophages from clinical and environmental samples
… universal marker gene present in all viruses. Metagenomics is a powerful tool that can be used to analyse viral communities in their natural environments. The aim of this study was to investigate diverse populations of uncultured viruses from clinical (a sputum of patient with cystic fibrosis, …
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Bioprospecting novel hemicellulases from the gut microbiota of the common black slug (Arion ater)
… able to degrade LCB. Of particular interest, metagenomic exploration of gut microbiota from invertebrates has yielded a significant resource. In this study, the output from the metagenomic analysis of the slug-gut microbiota was explored in search of novel enzymes targeting the hemicellulose …
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Efforts Toward Production of Novel Natural Products From Uncultured Soil Microbes
… New techniques emerging from the field of metagenomics take a culture-independent approach to examining the biosynthetic capabilities of soil bacteria by extracting the environmental DNA (eDNA) from the soil and cloning it into a host capable of maintaining and possibly heterologously …
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Identification and characterisation of lignin degrading bacteria and enzymes fromlarval guts of the African Palm Weevil (Rhynchophorus phoenicis)
… for novel lignocellulose degrading enzymes.Metagenomics allows access to the entire microbiome present in a particular environment and has been adopted in recent studies, rather than culture-based methods, thereby allowing for discovery of novel genes and enzymes from both culturable and …
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Expanding the Horizons of Enzybiotic Identification
… to tap into this diversity through functional metagenomic screens for lytic enzymes. Chapter 3 first addresses a preliminary methodological issue, namely the fact that uncultured phage samples generally do not provide sufficient quantities of DNA for ready screening. A novel ELASL protocol (for …
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… a deoxynivalenol detoxifying enzyme. To do this, library fragments from microorganisms was generated through oligonucleotide primed polymerase chain reaction (DOP-PCR) and transformed in a DON-sensitive yeast strain. Rounds of subculture were performed in the presence of DON and ferulic acid in …