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Showing 1 to 7 of 7 for “"metagenomic libraries"”.
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Natural Products From Functional Screening of Soil Metagenomic Libraries
… a strategy that eludes 99% of the microbiome. Metagenomics offers a route to study uncultured bacteria by extracting DNA present in environmental samples and cloning this eDNA into a vector to make metagenomic libraries. Libraries can then be screened in various ways to find natural products …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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Physiological effects of heterologous expression of proteorhodopsin photosystems
… PRPS previously identified from a large insert metagenomic library (Martinez et al., 2007). Incorporation of the PRPS into a strain of Pseudomonas putida resulted in a light-dependent increase in viable cell yield of cultures grown in low carbon media. The light-dependent effect demonstrates a …
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Recombination and Excision: DNA Repair Proteins in Prokaryotic Host-Virus Conflicts
… microorganisms using diverse bacterial metagenomic DNA libraries. These metagenomic libraries contain millions of DNA sequences from different microorganisms that are absent in available genetic databases. While bioinformatic mining has led to the discovery of many new bacterial immune …
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Functional metagenomic analysis of carbohydrate degrading enzymes from the human gut microbiota
… based on functional screening of genomic and metagenomic libraries using Escherichia coli and Lactococcus lactis as heterologous expression hosts, was used to isolate novel genes encoding glycoside hydrolase (GH) enzymes. The study identified several active GH enzymes involved in the breakdown …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… future. With the emergence of large-scale metagenomic sequencing projects, the number of protein sequences in databases has grown exponentially in recent years. This abundance of recorded sequence data, though, stands in contrast to the small number of experimentally verified functional …
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Isolierung von DNA und Konstruktion einer Metagenombank aus dem Sediment des Flusses Leine: partielle Sequenzierung und Annotation des Metagenoms sowie Analyse der mikrobiellen Diversität
Die Isolierung, Klonierung und Sequenzierung von Umwelt-DNA erlaubt eine Charakterisierung eines Standortes bezüglich seiner phylogenetischen und physiologischen Diversität, ohne dass eine häufig schwierige Anreicherung erfolgen muss. In dieser Dissertation wurde eine Probe vom Leinesediment …