Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"metabolomic analysis"”.
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Dissection of phloem transport in cucurbitaceae by metabolomic analysis
… mechanisms of Cucurbita maxima, by combining metabolomic analysis with cell biological techniques. This putative symplastic loading species has long been used for experiments on phloem anatomy, phloem biochemistry, phloem transport physiology and phloem signalling. Symplastic loading species …
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Metabolomic analysis of the interleukin-10-deficient mouse model of Crohn's disease
… immune tolerance towards intestinal microbiota. Metabolomic analysis is the study of small molecule metabolites, involving the measurement of large numbers of metabolites in biological samples. The aim of the research was to study the urinary metabolite profile of IL10-/- mice by gas …
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Genetic and metabolomic analysis of how population density modulates neuroendocrine physiology of C. elegans
In nature, organisms are presented with ever-changing environmental stimuli that can be beneficial or detrimental. Distinguishing the critically important environmental cues from the insignificant ones is integral to the survival of the organism. These cues may be based on chemical communication, …
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Metabolomic Analysis of Bacterial Cell Wall Intermediates Using LC-MS/MS: Peptidoglycan Pathway Response to Different Antibiotics
… intermediates in S. aureus. Quantitative analysis of UDP-linked intermediates is challenging, in part due to the lack of commercial available intermediates for use as standards. Therefore, we preparatively purified these intermediates from antibiotic treated bacterial cultures by HPLC, and …
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Interleukin-6 and Interleukin-10 concentrations as predictors of patient outcome following major traumatic injury
… and poorclinical outcome following trauma. Metabolomic analysis was also conducted in parallel tovalidate methodology and identify new molecules and pathways involved in the response totrauma. Interleukin-10 concentration was further utilised to cluster liquid chromatography/massspectrometry …
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Characterizing the early stages of a novel host shift using host fitness and metabolomics
… Drosophila C virus, host fitness assays and metabolomic analysis to measure the host’s response to a novel pathogenic infection. As Wolbachia can interfere with RNA virus infection success, a new method was developed to reliably and quickly eliminate Wolbachia from the flies. Once the flied …
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Evaluation of the mechanisms of neurotoxcity using metabolomic strategies
… proliferation assays coupled with LC-MS based metabolomics and transcriptomics techniques. Cells were treated with a range of cobalt concentrations between 0 and 200 μM. The metabolism of mephedrone at 100 μM was first studied in primary rat hepatocytes and then the neurotoxicity of mephedrone …
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Urinary and blood-derived biomarkers indicative of recovery from traumatic brain injury and spinal cord injury
… of patients and (b) that biological pathway analysis will provide an insight into the mechanisms behind the repair process. Methods: Patients with spinal cord injury and traumatic brain injury were recruited through the Foothills Medical Centre in Calgary, Alberta. Urine and blood samples …
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Multi-omic analysis of contaminated sediments from Contrecoeur, QC on Planorbid snails
… snails’ survival, growth, reproductive output, metabolomic profile, and proteomic profile. A two-week in-situ exposure was performed on Helisoma trivolvis, and a 28-d laboratory exposure of Planorbella pilsbryi to the same sediments was completed. Snail tissue samples were collected for …
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Metabolomic Signature of Hypertension
Metabolomics is a comprehensive study of small molecular end products of metabolism (metabolites) in living organisms. These metabolites are directly modified by the metabolic processes that are governed by organism’s genome, environment and diet. With advancement in technology, a large number of …
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Development of a Pediatric Model of Nafld in Neonatal Iberian Pigs
… relative gene expression, and Western blot analysis. Metabolomic analysis was performed on liver tissue and plasma. Body weight was not significantly greater in HFF fed pigs compared to CON. Leptin, alanine and aspartate aminotransferases, alkaline phosphatase, lactate dehydrogenase and …
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Metabolomics of desiccation tolerance in Xerophyta humilis
… This dissertation reports a high-throughput metabolomic analysis of the changes that occur in vegetative tissues of resurrection plant Xerophyta humilis during dehydration. A combination of chromatography, mass spectrometry and nuclear magnetic resonance revealed numerous primary and …
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Obesity, 11β-hydroxysteroid dehydrogenase and metabolic changes in the pathogenesis of idiopathic intracranial hypertension
… target tissues expressing 11β-HSD1. Metabolomic analysis identified a unique biofluid metabolite biomarker profile, with potential implications for IIH pathogenesis. We established the therapeutic efficacy of weight loss in IIH (improving headaches, papilloedema and ICP) and provided …
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TRYPTOPHAN-DERIVED MICROBIAL METABOLITES PROMOTE NKT22 PRO-TUMORIGENIC FUNCTIONS IN COLITIS ASSOCIATED COLORECTAL CANCER PATIENTS
… ex vivo and in vitro experiments were conducted. Metabolomics identified microbial-derived molecules activating iNKT cells. Mechanisms were studied using CAC murine models with or without functional iNKT cells. Increased NKT22 infiltration was observed in CAC tissues, along with enrichment of …
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A Nonribosomal Peptide Pheromone Controls Male-Induced Female Sexual Development in Schistosoma mansoni
… Using RNA sequencing, biochemistry assays and metabolomic analysis, we believe a male parasite starts to secret SmNRPS synthesized BATT into his pairing groove, upon copulation, to stimulate the paired female. The process is activated through transcription upregulation of the enzyme by GLI1. …
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Optimised spectral processing and lineshape analysis in 2-dimensional J-resolved NMR spectroscopy based metabolomics
… spectroscopy is a primary analytical approach of metabolomics. Although 1D 1H NMR spectroscopy is versatile, highly reproducible and widely used, analysis of complex biological samples yields congested spectra with many overlapping signals. This makes metabolite identification and quantification …
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Development and application of mass spectrometry-based metabolomics methods for disease biomarker identification
… to the metabolite level, leading to the study of metabolomics. The rise of metabolomics corresponds hand in hand with the desire to address some of the phenotypic informational gaps left behind from genomics, transcriptomics, and proteomics. The study of metabolites carries several advantages. …
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Survival potential of microalgae for long-term space missions
… Growth curves, microscopic images, pigment and metabolomic analysis were carried out on both radiated and control algal cultures to observe the effects of space radiation and desiccation on the algal cells in their different life stages.
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Regulation and roles of nutrient-dependent mitochondrial fusion
… mitochondrial fusion. Using a metabolomics approach, this project also studied changes in cellular metabolism during amino acid starvation-dependent mitochondrial fusion and how hyperfusion could crosstalk with these metabolic changes.;The results in this study indicated that …
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Early biomarkers to predict grade of encephalopathy following hypoxic ischaemic injury
… inflammatory proteins using multiplex assay; the metabolomics profile using LC/MS; and the miRNA profile using microarray. Results: Eighty five infants with perinatal depression were recruited. Analysis of inflammatory proteins consisted of exploratory analysis of 37 analytes conducted in a …
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