Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"metabolite analysis"”.
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Integrated cellular, proteomic and metabolite analysis of argyrin B with rational design approaches for immunoproteasome-selective inhibitors
… proteasome inhibitors through in silico analysis. Alongside biological studies of argyrin B cellular impact, proteomic effect and phase I metabolism. Potential interactions of 16 argyrin analogues at CP and immunoproteasome active sites were studied by molecular docking using Autodock …
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Comprehensive metabolite analysis in Chlamydomonas reinhardtii : method development and application to the study of environmental and genetic perturbations
This study introduces a method for multiparallel analysis of small organic compounds in the unicellular green alga Chlamydomonas reinhardtii, one of the premier model organisms in cell biology. The comprehensive study of the changes of metabolite composition, or metabolomics, in response to …
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Stopover behavior and refueling physiology in relation to migration distance in Wilson's Warblers (Wilsonia Pusilla)
… duration was estimated using mark-recapture analysis of color-banded birds, and relative refueling rate was measured using plasma metabolite analysis. Compared to short-distance migrant individuals, longer-distance migrants passed through later during spring migration, had shorter minimum …
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Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max
… Program, gene family search, multiple gene/metabolite analysis, motif analysis tool, protein 3D structure viewer and Cyber Studio system. It has a user-friendly web interface together with genome browser and pathway viewer, which display data in an intuitive manner to the soybean …
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The Physiological and Biochemical Understanding of 5’-Amp Induced Deep Hypometabolism
… Analyzing this data revealed many changes in metabolites. A few metabolites that stood out such as glucose and pyruvate did not alter the outcome in arousal rate over saline injection. Additional metabolite analysis revealed that an amino acid, arginine, was significantly reduced during DH. …
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Pathway optimization and engineering for biofuel production
… a 42% increase over the wild-type pathway. Metabolite analysis of the engineered pathway presented a 54% increase in cellobiose consumption (1.68 to 2.82 g cellobiose/(L•h)) and a 74% increase in ethanol productivity (0.59 to 1.03 g ethanol/(L•h)). By simultaneously engineering multiple …
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Effects of Brassica on the human gut microbiota
… sequencing identified these as L. fermentum. Metabolite analysis detected relatively high concentrations of lactate and short-chain fatty acids when faecal microbiotas were cultured in the presence of broccoli leachates, compared to a glucose control media. A human dietary study investigating …
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Combinatorial biosynthetic pathway engineering for microbial production of biofuels
… a 42% increase over the wild-type pathway. Metabolite analysis of the engineered pathway presented a 54% increase in cellobiose consumption (1.68 to 2.82 g cellobiose/(L•h)) and a 74% increase in ethanol productivity (0.59 to 1.03 g ethanol/(L•h)). The second half of the thesis was focused …
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Substrate analogs to investigate alkaloid biosynthesis in Catharanthus roseus
… organism for the production of secondary metabolite derivatives derived from these exogenous precursors. These studies examine precursor directed biosynthesis of alkaloid analogs in Catharanthus roseus hairy roots and seedlings. All terpene indole alkaloids are derived from tryptamine and …
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Understanding the molecular mechanism of postharvest seed coat darkening in pinto beans
… genetic complementation, transcript abundance, metabolite analysis, and inheritance study, I found a novel allele of P, Psd, whose partial loss-of-function slows down seed coat darkening by reducing the PA levels. Compared to P, Psd contains an additional glutamate residue and an arginine to …
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Energy and protein nutrition of ewes in late pregnancy
… daily and blood samples collected weekly for metabolite analysis. Lambs were weighed at birth, seven days old, approximately seven weeks old and at last at weaning. Supplemented ewes ate significantly less haylage than CTR ewes. Though treatment did not affect weight, BCS nor weight and BCS …
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Utilización combinada de técnicas analíticas y quimiométricas en lipidómica orientadas al descubrimiento de biomarcadores asociados a alteraciones en el metabolismo de lípidos
The research focused on metabolite analysis is an analytical discipline that is currently gaining much relevance thanks to the large amount of biological information from an organism that it reveals. The increasing knowledge of metabolic and lipid profiles has revealed the prominent role played by …
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Oxidative Phosphorylation: A Critical Feature and Novel Therapeutic Target In Melanoma Brain Metastases
… recently showed via RNA-sequencing (RNA-seq) analysis of clinical samples that melanoma brain metastases (MBMs) have higher expression of oxidative phosphorylation (OXPHOS) genes (including <em>PPARGC1A</em> or <em>PGC1α</em>) than patient-matched extracranial metastases (ECMs). <strong>Thus, …
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An Investigation into the utilisation of ethanolamine by uropathogenic E. coli
… Enterohaemorrhagic E. coli (EHEC). Transcriptome analysis of UPEC in active UTIs has found that the eut operon is expressed within the urinary tract and confers a competitive advantage in the murine urinary tract, but the exact mechanism conferring this advantage is not known. The aim of this …
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Metabolomic approaches to understanding the auxin and ethylene response in Arabidopsis roots
Non-targeted metabolite profiling by liquid chromatography-mass spectrometry (LC-MS) was used to determine the metabolite responses of Arabidopsis roots to auxin or ethylene. Crosstalk between these hormones regulates many important physiological processes in plants, including the initiation of …
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Is my eye okay with OK lens?: comparative in vitro study depicting the effects of Orthokeratology (OK) lens treatment on the physiology of human corneal epithelial cells
… estimate consumption and production of various metabolites. Cells were counted then lysed and lysate processed and the proteome analyzed using two-dimensional gel electrophoresis. In all treatments cell growth slowed or was arrested. Proteomic analysis fully identified 5 proteins – annexin A3, …
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Comparative analysis of desiccation responses in three Xerophyta species at two stages of seedling development
… in cellular ultrastructure, gene expression, and metabolite and lipid concentrations, the aim was to identify core conserved VDT mechanisms in these three Xerophyta species, as well as differences between poikilochlorophyllous and homoiochlorophyllous species in the same genus. Transcriptomic …
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Examining the roles of the pyruvate kinase isoforms, PKMI1 and PKM2, in systemic metabolism and tumor development
… PKM1. Aside from distinct changes in the plasma metabolite profiles of Pkm1-/- mice compared to wild-type (WT) mice, germline loss of PKMI appears to be of little phenotypic consequence. In contrast, PKM2 loss leads to a striking phenotype: spontaneous development of hepatocellular carcinoma …
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The Use of Spectroscopic Techniques in the Characterization of Mycobacterial Metabolites
<p>The mycobacterial cell wall metabolites have always imposed great challenges to researchers due to their unusual complexity and structural diversity. A lot of research efforts have been directed towards the evaluation of these metabolites and the role they play in the pathogenesis and virulence …
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Comparing diene derivatisation methods of dry blood spot samples for vitamin D metabolites quantification by liquid chromatography-tandem mass spectrometry
… quantification of biologically active vitamin D metabolites in limited volume serum and dry blood spot samples (DBS) using the liquid chromatography tandem-mass spectrometry (LC-MS/MS) analytical technique. This manuscript describes in detail the development and validation of an analytical …
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