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Showing 1 to 13 of 13 for “"metabolic labelling"”.
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To the Frontier of RNA Glycosylation: Novel Insights from Metabolic Labelling
… of this novel biopolymer was driven by the metabolic carbohydrate reporter, per-O-acetylated N-azidomannosamine (Ac4ManNAz), that emphasises the unknown potential of the field of glycobiology and RNA modification. However, the nascent field raises numerous questions concerning the molecule's …
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Novel fluorescent nucleotides for metabolic labelling and for the construction of DNA probes
Cílem této dizertační práce byla syntéza nových nukleosidů, nukleotidů a příslušných DNA sond nesoucích různé fluorescenční značky pro bioanalytické aplikace. V první části práce byl pomocí Sonogashiry cross-kapling reakce syntetizován 2'-deoxycytidin a jeho trifosfát nesoucí imidazolinový …
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The Development of Novel Tools for the Metabolic Labelling of Glycans in Cancer
… tagged monosaccharides. Enhanced in vitro glycanlabelling was observed using Ac3ManCyoc or Ac2ManCyoc relative to Ac4ManCyoc. This enhancement was consistent across multiple cell lines. Other tagged sugars showed reduced labelling with decreased acetylation, presumably due to decreased cell …
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From Probes to Cell Surface Labelling: Towards the Development of New Chemical Biology Compounds and Methods
… with regards to protein structure and function, metabolic processes and changes between healthy and diseased states. As an ever expanding field, developing new tools to interact with and impact biological systems is an extremely valuable goal. Herein, work is described towards the synthesis of a …
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Development of a Software Package for the Quantitative Analysis of Proteomic Mass Spectrometry Datasets Labelled with Nitrogen-15
Elemental metabolic labelling using 15N stable isotopes is a technique used in peptide-centric proteomics that allows samples to be mixed before preparation and analysis (minimising technical variance) without introducing sample ambiguity to the results. Labelling with 15N induces a mass shift in …
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Potential roles for transforming growth factor-B during terminal differentiation of ocular lens cells /
… lens annular pad (CLAP) cells. Immunoblot and metabolic labelling showed that TGF-β stimulation resulted in decreased synthesis of lens crystallins and no accumulation of a differentiation marker protein, phakinin. Measures of tritiated thymidine incorporation demonstrated that TGF-β …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… this chemical strategy could be combined with metabolic labelling to hijack the native RNA methylation machinery, allowing for the installation of a minimally perturbing allyl handle at endogenous modification sites for subsequent chemical ligation and transcriptome-wide analysis using Illumina …
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In vitro Expression of Human Zona Pellucida Protein 3
… using a specific anti-ZP3 antibody, and by a metabolic labelling study using <sup>3</sup>H-galactose. Several bands including a band at 65 kDa, were observed in both non-transfected and transfected medium by silver staining. Distinct bands were identified by Western blot analysis at 65 kDa and …
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Investigation of cystathionine β-synthase as a cause of mild hyperhomocysteinaemia in patients with peripheral vascular disease
… of defects in methionine metabolism, dual metabolic labelling of cultured fibroblasts with L-[methyl-³H]-methionine and L-[³⁵S]-methionine was developed to investigate these pathways in homozygotes and heterozygotes for CBS deficiency compared to controls. Although, no differences in the …
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The subcellular trafficking of the prion protein: Characterisation of the function of the PrPc N-terminus
… of PrPc in subcellular trafficking was analysed. Metabolic labelling and surface-biotinylation assays were performed in order to compare the intracellular trafficking and turnover of PrPc mutants showing specific deletions within their N-terminal sequence with those of wild type PrPc (wtPrPc). …
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Identification of bacteria-regulated mechanisms for the pathogenesis of inflammatory bowel diseases (IBD) and development of upconverting nanoparticle (UCNP) luminescence imaging for the monitoring of gut bacteria
… 2) to image the endogenous gut bacteria using metabolic labelling of bacterial peptidoglycan with azido-D-amino acids and strained-cyclooctynes such as Dibenzocyclooctyne (DBCO) -functionalised UCNPs. We showed a proof of principle for both these approaches using a fluorescent dye, but we were …
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Antibody Response to Heat Shock Proteins of Trypanosoma Cruzi and Histopathology in Mice Infected and Maintained at Elevated Environmental Temperature
… responses to parasite hsps were analyzed using metabolic labelling with S methionine/cysteine followed by immunoprecipitation, SDS-PAGE and autoradiography. The results showed that by day 15, serum from RT-infected mice recognized proteins of approximately 93kDa, 83kDa, and 66kDa, while in mice …
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Analyses of RNA dynamics in Mus musculus
… I developed a novel RNA sequencing method that metabolically labels RNA in a cell- and time-specific manner in mice and applied this method to solve biological problems that had been difficult to tackle. First, the robustness of the newly developed in vivo RNA labelling method was assessed. To …