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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"metabolic flux analysis"”.
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Metabolic flux analysis in mammalian cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1993.
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
Metabolic flux and population heterogeneity analysis were used to develop relations between mammalian cell physiology and specific culture environments and to formulate strategies for increasing cell culture performance. Mitochondrial characteristics associated with respiration, membrane potential, …
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¹³C-metabolic flux analysis of recombinant yeasts for biofuels applications
… lignocellulosic material or VFAs will require metabolic engineering of biocatalysts to improve yields, productivities, and final titers. These metabolic engineering efforts can be facilitated by ¹³C-Metabolic Flux Analysis (MFA), a method for elucidating the otherwise unobservable intracellular …
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Exploring cancer metabolism through isotopic tracing and metabolic flux analysis
… contemporary cancer research. Various types of metabolic alterations in cancer cells have been documented, prompting extensive investigation of the link between reprogrammed cell signaling pathways and rewired cellular metabolism. In addition, drug targeting of rewired metabolic pathways has …
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Nonstationary metabolic flux analysis (NMFA) for the elucidation of cellular physiology
… Many diseases for which we seek cures are metabolic in nature. Small-molecule biomanufacturing almost always involves metabolic engineering. Biofuels, a current topic of great interest, is essentially a metabolic problem. Even bioprocesses that involve complex products, such as enzyme or …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
<p>Metabolic engineering has evolved to the point of fulfilling the dream of having industrial chemicals produced renewably. Carboxylic acids [e.g., short chain fatty acids (SCFAs) such as octanoic acid (C8)] are such chemical intermediates that can be produced by Escherichia coli engineered with …
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Carbon-13 metabolic flux analysis of soybean central carbon metabolism: response to temperature and genotype effects
… for the changes in the composition are unknown;Metabolic flux analysis (MFA) quantifies carbon flow in a biological system, which is an important characteristic reflective of the system physiology. The application of MFA to study complex plant metabolic networks has however been recent. Towards …
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Identification of branch-point restrictions in microbial metabolism through metabolic flux analysis and local network perturbations
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1991.
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Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… microbes and rewiring the cellular functions in metabolic engineering. Complementing the genomics, transcriptomics, proteinomics and metabolomics analysis of microbial metabolism, fluxomics tools can measure and simulate the in vivo enzymatic reactions as direct readouts of microbial metabolism. …
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Fermentative utilization of glycerol and lignocellulosic sugars and production of ethanol by Paenibacillus macerans
… production of ethanol by P. macerans. Further, metabolic flux analysis (MFA) was used as a systemwide tool to understand the role of various metabolic pathways in the fermentative utilization of these substrates. Glycerol is a byproduct of biodiesel and bioethanol production, which is an …
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Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
Metabolic Flux Analysis (MFA) has emerged as a tool of great significance for metabolic engineering and the analysis of human metabolic diseases. An important limitation of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need …
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Methodologies and analysis of metabolic flux in mammalian systems
<p>Quantification of metabolic fluxes has broad applications in studying metabolic physiology. Isotope tracing with heavy labeled carbon (13C labeled metabolic flux analysis, 13C-MFA) is a promising strategy due to its ability to compute metabolic fluxes from isotope tracing profiles without …
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Optically pure D (-) lactic acid biosynthesis from diverse renewable biomass: microbial strain development and bioprocess analysis
… acid from biomass sugars; and 5) to perform metabolic flux analysis of carbon flow in Lactobacillus strains used in our study. Our results showed that D-lactic acid yield from alkali-treated corn stover by L. delbrueckii and L. plantarum NCIMB 8826 ∆ldhL1 via SSF were 0.50 g g[superscript]-1 …
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Physiological Oxygen and Media Conditions Influence Breast Cancer Hallmarks in Vitro
… enhancing cell migration, glucose uptake, and metabolic activity. Additionally, I developed and optimized a novel protocol for performing Seahorse metabolic flux analysis under hypoxic conditions, enabling more accurate assessment of metabolism in low-O₂ environments. Collectively, our results …
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Study of the differential compartmentation and functions of hexokinase isoforms and implications for cancer metabolism
… targeting cancer-specific isoforms of key metabolic enzymes is a very promising strategy. Elevated glucose consumption and lactate secretion under aerobic conditions is a hallmark of many cancer cells. One of the major contributors to this highly glycolytic phenotype is hexokinase 2 isoform …
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Development of 13C Fingerprint Tool and Its Application for Exploring Carbon and Energy Metabolism in Cyanobacterium Synechocystis sp. PCC 6803
… will provide guidelines for rational design of metabolic engineering strategies.</p><p>The emerging of multiple omics tools, e.g. genomics, transcriptomics, proteinomics, and metabolomics analysis, allowed the comprehensive determination of microbial metabolisms. This dissertation describes the …
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Quantifying the Intracellular Metabolic Network that Establishes the Simultaneous Utilization of Sugars and Aromatic Substrates in Pseudomonas putida KT2440
… a combination of 13C tracer experiments with metabolic flux analysis (MFA), I elucidated and quantified the discriminate metabolic routing of each substrate throughout central carbon metabolism. The results determined that glucose-derived carbon was primarily routed to the Entner-Doudoroff …
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The accuracy of linear flux models in predicting reaction rate profiles in a model biochemical reaction system
Metabolic flux analysis is commonly used in the modelling of biochemical reactions. The use of MFA models has gained large amounts of interest due to the simplicity of the computational procedures required for the model, and the exclusion of difficult to measure intracellular reaction data. There …
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The Role of Pyruvate Dehydrogenase in Cell Growth
… in vitro using a combination of metabolomics and metabolic flux analysis. Growth in monolayer culture was performed in medium containing lipid-replete serum as well as serum in which lipids had been depleted. As expected, suppression of PDH activity reduced flow of carbon from glucose to the TCA …
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Expression, Production, and Purification of Novel Therapeutic Proteins
… cellular level.</p> <p>The second model utilized metabolic flux analysis to track changes in metabolite concentrations and biomass during the expression of the target protein. Upon solving this model, the prediction of the intermediate fluxes proved to be accurate for glucose-fed experiments, as …
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