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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 143 for “"messenger RNA"”.
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FMRP dependent synaptic delivery of messenger RNA
… regulatory role of FMRP involved in synaptic mRNA delivery. In chapter 1, the molecular mechanism of local protein synthesis and synaptic mRNA delivery and its roles in Fragile X syndrome and learning and memory are introduced. In chapter 2, my data demonstrated that FMRP can facilitate mRNA …
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF signals and show that they promote significant mRNA destabilization through the nonsense …
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Complementary DNA to sea urchin histone messenger RNA
A preparation of 9S RNA was isolated from early blastula sea urchin embryo polyribosomes by phenol extraction followed by repeated sucrose density gradient centrifugation. When incubated in an ascites cell free protein synthesizing system this preparation of RNA was shown to support the synthesis …
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Engineering therapeutic messenger RNA for triple-negative breast cancer
… was limited by the use of viral vectors. Messenger RNA (mRNA) technology has been cemented as a safe and effective therapeutic modality, as evidenced by its success in SARS CoV-2 vaccination. Its potential to revolutionise cancer therapy is now becoming increasingly evident. We …
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Messenger Rna Profiling: A Prototype Method For Body Fluidand Tissue Identification
… the presence and relative abundance of specific mRNA species. If the type and abundance of mRNAs can be determined in a stain or tissue sample recovered at the crime scene, it would be possible to definitively identify the tissue or body fluid in question. Advantages of an mRNA-based approach, …
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Protein-dependent prediction of messenger RNA binding using Support Vector Machines
RNA-binding proteins interact specifically with RNA strands to regulate important cellular processes. Knowing the binding partners of a protein is a crucial issue in biology and it is essential to understand the protein function and its involvement in diseases. The identification of the …
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USING ANTISENSE MESSENGER RNA TO DOWNREGULATE LON MEDIATED PROTEOLYSIS IN ESCHERICHIA COLI
… We examined the ability of lon antisense RNA to inhibit the production of endogenous lon protease and increase the protein yield and activity of a model recombinant protein, organophosphorus hydrolase (OPH). Results indicate that the overproduction of lon antisense did effectively …
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The Role of Phosphorylation In Pam2 Motif-Containing Proteins Mediated Messenger Rna Deadenylation
… many cellular processes. However, its role in mRNA deadenylation, a process to remove poly adenosines from the mature mRNA 3’ end tail, is unclear. The length of poly(A) tail determines mRNA stability and translation efficiency. Poly(A)-binding protein (PABP), which binds to newly synthesized …
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Estrogen Regulates Vitellogenin Gene Transcription and Messenger-Rna Degradation in Xenopus Laevis Liver
Vitellogenin gene transcription and mRNA degradation rates were measured in Xenopus laevis liver using pulse labeling and pulse chase techniques. Estrogen regulates the accumulation of vitellogenin mRNA through three processes: (1) There is a selective transcriptional activation of the vitellogenin …
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A METHOD FOR IDENTIFYING RIBOSOME PAUSE SITES IN MESSENGER RNA THROUGH NEW SEQUENCING TECHNOLOGY
… of this process. Mechanisms, such as aminoacyl-tRNA selection and codon:anticodon interactions, are well characterized. However, more complex mechanisms, such as mRNA secondary structures, require a new approach for further study. We suggest that these mechanisms may be studied on a global scale …
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Cloning and Estrogen Regulation of the Retinol Binding Protein Messenger-Rna of Xenopus Laevis
… full length cDNA clone for an estrogen induced mRNA isolated from the liver of Xenopus laevis. By DNA sequencing and subsequent homology alignment with the sequence for human retinol binding protein, the clone was identified as the mRNA for serum retinol binding protein of Xenopus. Since we …
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Expression of dsr messenger RNA by sulfate reducing bacteria with varying substrate and temperature
… reducing bacteria by measuring the number of messenger RNA (mRNA) transcripts of dsr, a critical gene in the sulfate reduction pathway, in a cell. Because conditions in aquifers can be quite different from those maintained in laboratory experiments, it is unknown whether the results of such …
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Initiation of Translation of Eukaryotic Messenger-Rna at a Unique Site Lacking an AUG Codon
The initiation of translation of eukaryotic mRNA is believed to proceed by a "scanning mechanism" where the ribosome recognizes a 5$\sp\prime$ terminal cap structure (m$\sp7$GpppN ...) and then scans down the mRNA until reaching the first 5$\sp\prime$ terminal AUG or $\sp{\rm A}\sb{\rm …
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Transfer rna dynamics and transfer-messenger rna accommodation in bacterial ribosomes at the single-molecule level
… biochemical tools were applied to study transfer RNA (tRNA) dynamics and transfer-messenger RNA (tmRNA) accommodation inside ribosomes. In the first project of this thesis work, structural characterization of the tRNA-like domain of tmRNA (TLD) in complex with SmpB protein was carried out, and the …
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The Effect of Capping Upon The Messenger-Rna Properties of Satellite Tobacco Necrosis Virus Ribonucleic Acid
Made available in DSpace on 2014-12-14T04:27:49Z (GMT). No. of bitstreams: 1 8009175.pdf: 3660669 bytes, checksum: 3db92f5482e7bdc2e20a68a90694ac32 (MD5) Previous issue date: 1979
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Network Analysis and Comparative Phylogenomics of MicroRNAs and their Respective Messenger RNA Targets Using Twelve Drosophila species
MicroRNAs represent a special class of small (~21–25 nucleotides) non-coding RNA molecules which exert powerful post-transcriptional control over gene expression in eukaryotes. Indeed microRNAs likely represent the most abundant class of regulators in animal gene regulatory networks. This study …
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Design, synthesis, and biological evaluation of diketopiperazine based ionizable lipids for the in vivo delivery of messenger RNA
… of specific proteins. The successful delivery of messenger RNA (mRNA) to target cells in the body could accomplish this goal, but serious limitations with its systemic delivery must still be overcome. Recently, lipid nanoparticles (LNPs) have shown promise for mRNA delivery in vivo, however, …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
… are involved in gene expression---transcription, RNA processing, and translation. To expand our knowledge of these processes in Archaea, I have studied transcriptional promoters, messenger RNA processing, and 5'-untranslated regions in Methanocaldococcus (Methanococcus) jannaschii. The promoters …
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