Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"membrane-spanning"”.
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Design and Synthesis of a Macrocyclic Phospholipid
The membrane-spanning phospholipids of the domain Archaea are postulated to provide membrane stability; this thesis reports the design and synthesis of a synthetic membrane-spanning macrocyclic lipid to test this hypothesis. Protected glutaric anhydride reacted with 10-undecyn-1-ol to produce a …
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DNA-Based Mimics of Membrane Proteins Lipid-DNA Interactions Determine Function
… synthetic constructs mimicking function of membrane proteins. These natural molecular machines function embedded in the lipid bilayer. Similar membrane attachment of DNA-based structures is achieved by modifying the nucleic acid with hydrophobic anchors, most commonly cholesterol. Aiming at …
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Novel Insights into the Role of the Smoothened Cysteine Rich Domain in Hedgehog Signalling
… The structure of the Smo receptor includes seven membrane spanning domains, extracellular and intracellular loops connecting the membranous domains and the extracellular cysteine rich domain (CRD). The extracellular CRD of the Smo receptor is homologous to the Frizzled (FzD) CRD. The FzD CRD …
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A Transmembrane Precursor of Secretory Component
… as soluble secretory forms, but as larger transmembrane forms that are core glycosylated and asymmetrically integrated into the dog pancreas microsomal vesicles with an 11-15 kilodalton domain remaining in the cytoplasm. We studied the biosynthesis and processing of human SC in a cell-free …
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A Search for the Counter-Immune Mechanisms of Mycobacterium Tuberculosis
… pks6/Rv0405 - a polyketide synthase, Rv0072 - a membrane spanning domain of a putative glutamine transporter, Rv2958c - a glycosyl-transferase, and pstA1/Rv0930 - a membrane spanning domain of an inorganic phosphate transporter. Monotypic IV infections confirmed that these mutants replicate …
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Localization of a quinol oxidase domain of the cytochrome d complex of Escherichia coli
… oxidation of ubiquinol-8 within the cytoplasmic membrane and the reduction of molecular oxygen to water. Both oxidases are coupling sites in the respiratory chain. The cytochrome d complex is a heterodimer (subunits I, II) which has three heme prosthetic groups. The hydropathy profiles of the …
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Characterization and transcriptomic profiling of Arabidopsis thaliana under cellulose biosynthesis inhibitor treatment
… models and predicted a protein with seven-membrane spanning helices. Transcriptomic profiling of Arabidopsis thaliana in response to CBI treatment revealed differential expression of genes related to membrane dynamics, stress responses, cellular transporters, and general metabolism. …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
… via a concerted effort by a variety of cell membrane spanning amino acid transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design and synthesis of amino acid analogs, based off of an L-aspartate or L-proline scaffold, to be evaluated …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
… via a concerted effort by a variety of cell membrane spanning amino acid transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design and synthesis of amino acid analogs, based off of an L-aspartate or L-proline scaffold, to be evaluated …
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Cloning and regulation of rat and Xenopus laevis hepatic 3-hydroxy-3-methylglutaryl Coenzyme A reductase
… of amino acid sequence conservation. The seven membrane spanning domains exhibit the highest amino acid sequence similarity, while the linker region displays the lowest level of amino acid homology.
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Rational Design of DNA-Based Lipid Membrane Pores
… In this thesis, we use DNA to build synthetic membrane-inserting channels. Porphyrin and cholesterol tags serve as membrane anchors to facilitate insertion into the lipid membrane. With atomic force microscopy, confocal imaging and ionic current recordings we characterise our DNA nanochannels …
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Molecular cloning, characterization, and expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase gene from tomato
… 602 amino acid residues (about 64,714 Da). Two membrane-spanning regions are contained in the NH₂-terminus of the HMGR polypeptide. The COOH-terminus shares significant homology with HMGR sequences from different species. Genomic Southern hybridization analyses reveals that tomato contains 3 to …
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Vergleichende Analyse der Gerstmann-Straeussler-Scheinker-Syndrom-assoziierten Mutation A117V mit der neuen pathogenen Mutation G114V des humanen Prion-Proteins in vivo und in vitro
… the prion protein (PrP) can exist in two transmembrane forms (NtmPrP and CtmPrP), which span the lipid bilayer in either direction. Certain mutations in the membrane-spanning segment of PrP have been shown to increase synthesis of CtmPrP and result in neurodegeneration. One of these mutations …
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Role of the Aspartyl Protease SpoIIGA in the Compartmentalization of Sigma Factor Activation During Sporulation of Bacillus subtilis
… protein is exported, and localizes to the inter-membrane space of the sporulation septum, which may be mediated directly by SpoIIGA. SpoIIR and SpoIIGA are essential for the activation of σE from its inactive precursor, pro-σE. Pro-σE, encoded by spoIIGB, is part of a two-gene operon that also …
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Novel Strategies towards the Inhibition of ATP-Binding Cassette (ABC) Transporters from Pathogenic Bacteria
… of lipids that form antibiotic impervious membrane structures. Identifying novel antibiotic targets and strategies is required to address the ever-growing antibiotic crisis. ABC transporters are an untapped pool of potential targets. Two examples are the essential lipid transporter MsbA, …
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The roles of Cabin1 and Left1 in T cell development
… CD8+ T cells, we identified a member of the membrane spanning 4A gene family, MS4a4b, which we named Leftl. It was also found to be expressed in Thl, but not Th2 cells. To examine the role of Leftl in T cell development, we created transgenic mice which express Leftl in T cells. These mice …
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Structure, Function and Dynamics of G-Protein coupled Receptors
Understanding the function of membrane proteins is crucial to elucidate the molecular mechanisms by which transmembrane signaling based physiological processes,i. e., the interactions of extracellular ligands with membrane-bound receptors, are regulated. In this work, synthetic transmembrane …
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Kinetics and specificity of nicotinamide nucleotide binding to the dIII component of transhydrogenase from Rhodospirillum Rubrum
… is an enzyme located in the cytoplasmic membrane of bacteria or the inner membrane of animal mitochondria. Using the energy of the proton electrochemical gradient (Δp), transhydrogenase translocates protons across the membrane whilst undergoing its redox reaction, in which hydride ion …
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Molecular Studies of the Succinate:ubiquinone Oxidoreductase of Escherichia Coli
… oxidoreductase (Complex II) is a membrane-bound enzyme present in aerobically grown bacteria and in the mitochondria of eukaryotes. It functions as succinate dehydrogenase in the citric acid cycle and transfers reducing equivalents to ubiquinone in the aerobic electron transport …
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H36-alpha7 is a novel integrin alpha chain that is developmentally regulated during skeletal myogenesis
H36 is a 120,000 Da membrane glycoprotein that is expressed during the differentiation of skeletal muscle. H36 cDNA clones were isolated from a lambda UniZapXR rat myotube cDNA library and sequenced. The deduced amino acid sequence demonstrates that H36 is a novel integrin alpha chain that shares …
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