Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"membrane traffic"”.
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A Chemically Induced Colitis Screen Reveals the Necessity for Membrane Traffic in Intestinal Homeostasis
… by CRISPR/Cas9 targeting. Three vesicle trafficking genes, Myo1d, Smcr8, and Tvp23b, were selected for mechanistic evaluation. MYO1D is a class I myosin that binds both actin and lipid. MYO1D localizes to the basolateral membrane of enterocytes and functions in the intestinal epithelium …
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Phagocytosis by neutrophils - studies on phagosome dynamics and membrane traffic modulation by Streptococcus pyogenes
… where they eat and enclose their target inside a membrane-bound organelle, the phagosome. Neutrophil phagosomes are highly dynamic entities, and a large amount of antimicrobial substances are released to their interior within seconds of formation. In most cases this will kill the engulfed microbe, …
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The Interplay of Signal Transduction and Membrane Traffic of the Macrophage Colony Stimulating Factor Receptor (MCSFR) Controls Macrophage Growth
… leukemias. Clearance of RTKs from the plasma membrane is an important mechanism for regulating the signal amplitude, however, the exact mechanisms remain elusive. To better define these mechanisms, we characterized MCSFR internalization, endocytic trafficking, and signaling regulation by new …
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Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled …
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The function of golgin vesicle tethers in Drosophila melanogaster
Accurate membrane targeting is essential for the organisation of membrane traffic. This step is particularly crucial at the Golgi apparatus, a central sorting hub of membrane traffic in the cell. Golgins, a class of highly conserved coiled-coil vesicle tethers, have been shown to mediate …
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Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi
Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of …
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An evolutionarily conserved regulatory mechanism for endosomal membrane trafficking
Membrane fusion in all eukaryotic cells is facilitated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes; a process that is regulated by Sec1p/Munc18 (SM) proteins. Membrane fusion has been conserved through evolution and hence a lot or our …
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The interconnected roles of spastin and protrudin in endosomal sorting
… with endoplasmic reticulum (ER)-endosome membrane contact sites and several ER proteins are required for efficient endosomal tubule fission. Our lab has previously identified two hereditary spastic paraplegia (HSP)- associated proteins, spastin (SPG4) and atlastin-1 (SPG3A), as essential …
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Analysis of the role of rab11-FIP3 phosphorylation during cytokinesis
… two daughter cells, thus completing mitosis. Membrane traffic is at the heart of mammalian cytokinesis. Rab11-FIP3 (also referred to as FIP3) in complex with Rab11 plays a key role in the delivery and targeting of recycling endosomes to the furrow; this is essential for completion of …
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Primordial imperatives and the evolution of control of endolysosomal trafficking
… to changes in osmotic conditions to maintain the membrane, which protects life from the harsh realities of the second law of thermodynamics. The primordial imperatives of the first and second law of thermodynamics underlie the evolution of the processes regulating membrane traffic. In order to …
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Structure/Function Studies on Vps9 domain-containing Ankyrin Repeat protein (VARP)
… SNX proteins to mediate both Endosome to Golgi traffic, and Endosome to Plasma membrane traffic. The interaction of Varp with Vps29 localises Varp to endosomal membranes. Varp has two ‘zinc knuckle’ sequences (residues 369- 460 and 692-746) that have been shown to bind zinc and it is these small …
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Profilin II : novel functions in membrane trafficking
Profilin II - Novel functions in membrane-trafficking Ralph Gareus - EMBL Mouse Biology Programme, Monterotondo, Italy Profilin is an actin-binding protein expressed in all eukaryotic cells, regulating microfilament remodelling. Besides actin, it binds to poly-L-proline and phosphatidylinositol …
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Membrane Surface Charge and Lipid Signaling in Phagocytosis
… transduction, cytoskeletal reorganization and membrane remodeling. Lipids are increasingly appreciated to play a crucial role in these events. Sphingolipids, cholesterol and glycerophospholipids, notably the phosphoinositides, are required for the segregation of signaling microdomains and for …
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Differential Regulation of Phosphatidylinositol 4-Phosphate 5 Kinase Beta during Hypertonic and Oxidative Stress
… cellular processes such as signal transduction, membrane traffic, actin cytoskeleton, ion homeostasis, growth and apoptosis. Despite the importance of this signaling molecule in controlling a diverse array of functions, little is known about the regulation of the kinases that produce PIP2 as well …
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Lysosome biogenesis during osteoclastogenesis
… mechanisms responsible for lysosomal enzymes traffic. Osteoclasts are bone-degrading cells. To perform this specific task they have to reorganise the sorting of their lysosomal enzymes to be able to target them toward the bone surface in mature cells. Since few years, the differentiation of …
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Hexagonal packing of Drosophila wing epithelial cells by the Planar Cell Polarity pathway
… polarity proteins may modulate Dynamin-dependent trafficking of E-cadherin to enable the dynamic remodeling of junctions. We furthermore show that the Planar Cell Polarity protein Flamingo can recruit the exocyst component Sec5. Sec5 vesicles also co-localizes with E-cadherin and Flamingo. Based …
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Organization and formation of the apical membrane of epithelial cells
Compartmentalization of cell membranes, in particular of the apical membrane of columnar epithelia, is the topic of this thesis. The first part characterizes the apical membrane and its specialized organization and morphology, whereas the second part focuses on the formation of this unique plasma …
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Regulation of sodium channel densities in A6 renal epithelial cells by hormonally-dependent mechanisms
… sodium channels (ENaC) located in the apical membranes of transporting epithelia, such as cells of the renal distal nephron, constitute the rate limiting step in the transcellular transport of sodium. The regulation of sodium ion reabsorption in the kidney, which is essential for salt and …
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Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… pathogen modification of the vacuolar inclusion membrane and/or to selective recruitment or exclusion of host trafficking regulators. As a result, the A. phagocytophilum-occupied vacuolar membrane (AVM) provides a unique interface to study the host-pathogen interactions critical to A. …
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
Vesicular traffic along the biosynthetic and endocytic pathways is essential for homeostasis of eukaryotic cells. However, it raised the question of how the proteins characteristic for each compartment are transported to their destination (Bonifacino and Glick, 2004). This study is especially …
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