Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"membrane topology"”.
-
Investigation of Prestin Membrane Topology by Substituted Cysteine Accessibility Method.
Prestin, an integral membrane motor protein in the outer hair cells of the inner ear, is proposed to be crucial for cochlear amplification. In support of this, in prestin knock out mice, there is a 40-60 dB loss of hearing sensitivity. Little is known about prestin’s structure-function …
-
The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
"In order to characterize this secondary-binding interaction, a novel surface plasmon resonance technique termed ""analyte gradient-surface plasmon resonance"" (AG-SPR) has been developed and is described here. AG-SPR can be used to rapidly characterize macro-molecular interactions without …
-
PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
… glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight around 61 kD. …
-
PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
… glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight around 61 kD. …
-
The structure of a voltage gated potassium channel
… for regulating the potassium component of the membrane potential in a large range of cell types ranging from mammalian excitable cells to bacteria. Attempts have been made to elucidate the structure of voltage gated potassium channels using X-ray crystallography, however due to the inherent …
-
Investigation and control of dermal fibroblast signaling during injury repair
… characterized a novel actin cytoskeleton-plasma membrane topology present in human dermal fibroblasts at the extreme end of cellular contractility. We also developed several technical advances: the real-time imaging and manipulation of calcium in 3D microtissues, the development of a reporter for …
-
Analysis of the streptococcal cpsa protein in dna-binding and regulation of capsule and cell wall maintenance
… pathogens is controlled in part by the membrane bound protein CpsA. These studies analyze the contribution of CpsA to regulation of capsule level in the model aquatic pathogen Streptococcus iniae and human specific pathogen GBS, and how this regulation affects virulence in in-vitro, …
-
SCAP, Insig, and Cholesterol Interactions in Mammalian Cells
… release of SREBPs from their inactive membrane bound form. Soon after translation, SREBPs bind SREBP cleavage activating protein (SCAP), a polytopic membrane protein of the endoplasmic reticulum (ER). In sterol depleted situations, SCAP escorts SREBPs to the Golgi, where SREBPs are …
-
Structure-function studies of human SLC36 amino acid transporters
… Linked Carrier family 36 (SLC36) of integral membrane transport proteins has four members which are also known as the human Proton coupled Amino acid Transporters (hPATs).This is due to hPATs 1 and 2 having been functionally characterized as proton coupled transporters that bring about a 1:1 …
-
A combined threading and genetic algorithm based approach to predict protein structure
… in prediction of structures for helical transmembrane proteins. Here we combine a systematic plot of the Ramachandran protein conformation map with our existing application. This setting allows us to generate models with correct membrane topology. However, further investigation will be …
-
Mutagenesis and Spectroscopic Studies on Cytochrome Bd Quinol Oxidase of Escherichia Coli
… conclusive experimental results for a new membrane topology model of subunit I, in which the binuclear center is relocated to the periplasmic side of the membrane. A proton channel now becomes evident so that protons can access the binuclear center and the water resulting from oxygen …
-
Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the GP-C complex, with emphasis on elucidating the role of SSP in arenavirus life cycle. We present genetic evidence that support the inclusion of G2 …
-
Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the GP-C complex, with emphasis on elucidating the role of SSP in arenavirus life cycle. We present genetic evidence that support the inclusion of G2 …
-
Functional and structural analyses of an olfactory receptor from Drosophila melanogaster
… is mediated by a large family of integral membrane proteins, called olfactory receptors (ORs), that mediate the transduction of odorant binding into a neuronal signal. A functional assay for insect ORs was developed utilising calcium imaging in Sf9 cells. The Drosophila melanogaster OR, …
-
HAMP Domain and Signaling Mechanism of the Aer Protein
… in the C terminus, separated by a putative transmembrane region. In this dissertation, I report that the linker region of Aer (residues 205-265), which joins the transmembrane region to the signaling domain, is a conserved signal transduction module in numerous methyl-accepting chemoreceptors, …
-
Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… for C. difficile and P. sordellii share similar membrane topologies to that of the phage lambda holin. However, TpeE in C. perfringens shares a similar membrane topology to TatA, a key protein in the Twin Arginine Transport (Tat) secretion system. This thesis tests two models, a pore-forming …
-
Membrane Domain of Plant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: Targeting, Topology, and Function
… exon I of tomato hmg1 which encodes the putative membrane domain. Sequence comparisons of plant HMGR proteins reveal two hydrophobic stretches within the amino terminus which are highly conserved among species. Using in vitro transcription and translation systems, the membrane domain structure of …
-
The little engine that could: Characterization of noncanonical components in the speed-variable flagellar motor of the symbiotic soil bacterium Sinorhizobium meliloti
… provide increased contact points in the inner membrane, the peptidoglycan sacculus, and, in Gram-negative bacteria, the outer membrane. Additionally, the soil-dwelling bacterium Sinorhizobium meliloti exhibits marked distinctions in the regulation, structure, and function of its navigation …
-
Molecular mechanisms of KRas4b signaling
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01