Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"membrane permeabilization"”.
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Mechanisms of Adenovirus Membrane Permeabilization
… to occur, a virus must first penetrate host cell membranes to access intracellular sites of viral replication. Currently the mechanism through which adenovirus, a non-enveloped, dsDNA virus, disrupts the endosomal membrane during cell entry is not well characterized.</p><p>Recent studies suggest …
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NEW INSIGHTS INTO THE REGULATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION DURING APOPTOSIS
… proceeds in a linear fashion. Mitochondria outer membrane permeabilization (MOMP) and the release of intermembrane space proteins (e.g., cytochrome c) are early events during mitochondria-mediated apoptotic signaling. In addition, Apaf-1 is a critical component of the mitochondrial pathway and is …
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
… We demonstrate in numerous ways that after permeabilization the cells remained viable and responded normally to cell signaling protein. We applied this technique to observe the dynamic motion of labeled actin and mitochondria using super-resolution fluorescence microscopy (dSTORM). …
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Pseudomonas aeruginosa Type IV pilus and resistance to the antimicrobial properties of surfactant protein-A
… microbial clearance by opsonization and membrane permeabilization. Pseudomonas aeruginosa (PA) is a major Gram-negative bacterial pathogen commonly associated with chronic lung infection in cystic fibrosis. In order to resist the antibacterial effects of SP-A, PA expresses various …
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Electronic tuning of site selectivity and semisynthesis of C2'-deoxyamphotericin B
… self-assemble into ion channels in phospholipid membranes containing sterol. The resulting membrane permeabilization has long been the leading hypothesis for the primary mechanism of antifungal activity. Three possible interactions involving the carboxylic acid and mycosamine appendages of AmB …
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Interactions of shiga-like toxin-2 (Stx-2) from Escherichia coli O157:H7 and the Bcl-2 family of proteins during host cell programmed cell death
… family members such as Bak at the mitochondrial membrane therefore inducing mitochondrial membrane permeabilization (MMP) and its downstream effects.</p>
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Characterization of a Mitochondrial Localizing Molluscum Contagiosum Virus Protein: MC163R
… metal binding domain, a transmembrane domain and a large C-terminal domain that contains no identified homology to other viral or host proteins. Confocal microscopy was utilized to verify the predicted mitochondrial localization. MC163 was detected at the mitochondria, whereas …
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… to create pores along the bacterial cell membrane. Current methods for assessing pore formation do not directly measure cell permeabilization or enable same-day assessment. In this thesis, a novel fast-screening protocol combining SYTOX green, microfluidics, and fluorescence imaging is …
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Origins of cell-to-cell variability in apoptosis
… arose upstream of mitochondrial outer membrane permeabilization (MOMP), with little contribution from downstream reactions. More specifically, the rate of cleavage of initiator caspase substrates was highly predictive of a cell's death time. However, to determine whether (as opposed to …
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Naujo tetrakatijoninio porfirino sukeltos Escherichia coli fotopažaidos /
… and selected functions of E. coli KMY1 cell membranes. It was shown that tetracationic TN-Et-PyP symmetry changes strongly depend on the polarity of the solvent. The dye forms complexes with nucleic acids and lipopolysaccharides in buffer solution, the interaction being of electrostatic …
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A molecular investigation of the antimicrobial functions of the human S100 host-defense proteins
… factor that functions through a mechanism of membrane permeabilization. Taken together, our studies provide new insights into the multifunctionality of the antimicrobial S100 HDPs.
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Exploring the Activation Landscape of Pro-Apoptotic BAK Through the Discovery of Human BH3-Only and Non-Native Peptide Binders
… pro-apoptotic BAK leads to mitochondrial outer membrane permeabilization (MOMP), release of cytochrome c, and ultimately cell death. The BH3 binding event leads to a series of conformational changes that promote the conversion of BAK from monomer to dimer and subsequently to oligomers that …
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The role of the retinoblastoma protein in mitochondrial apoptosis
… activate BAX, resulting in mitochondrial outer membrane permeabilization and apoptosis. Importantly, targeting ectopically expressed pRB specifically to the mitochondria generated a separation-of-function mutant deficient for pRB's classic, nuclear roles. Remarkably, we found that this …
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Computational and Experimental Investigation of the Critical Behavior Observed in Cell Signaling Related to Electrically Perturbed Lipid Systems
… directly impact the intracellular mitochondrial membrane at clinically relevant field amplitudes. As demonstrated in this work, PEFs may influence the mitochondria via an indirect route such as disruption of the actin cytoskeleton and/or alteration of ionic environment in the cytoplasm due to …
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Characterization of the Bacteriophage Felix O1 Endolysin and Potential Application for Salmonella Bioremediation
… and a second agent to permeabilize the outer membrane of the bacterium. The holin protein was proposed as an outer membrane permeabilization agent. Methods used to locate the holin gene included BLAST analysis, analysis of putative Felix O1 proteins for transmembrane domains, and examination …
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Characterization of the Helicobacter pylori vacuolating cytotoxin mediated induction of mitochondrial cell death mechanism
… translated to Bax mediated mitochondrial outer membrane permeabilization through the stress sensor Bid. Furthermore, Drp1 dependent mitochondrial fission also resulted in the increase in cytosolic calcium levels, which was required for the activation of the calcium dependent cysteine protease …
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Multimodal Optical Interfaces Enabled by Multiresonant Plasmonics for Bio-Nanophotonics
… enables highly localized, minimally invasive membrane permeabilization—optoporation—facilitating intracellular biochemical sensing and molecular delivery with nanoscale precision. Such capabilities hold significant promise for applications in bio-nanophotonics, targeted drug delivery, and …
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Regulation of BAX/BAK-Dependent Cell Death Program
… BAX/BAK promotes mitochondrial outer membrane permeabilization, whereas anti-apoptotic BCL-2/BCL-X<sub>L</sub>/MCL-1 protects mitochondrial integrity and prevents cytochrome c release. The proapoptotic activity of BAX/BAK is triggered by BH3-only molecules: BH3s) which are upregulated …
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Endosomal Trafficking as a Determinant of Antifungal Tolerance in the Pathogenic Fungus Candida albicans
… fluidity and permeability of fungal cell membranes. These include the azole antifungals, which block ergosterol biosynthesis by inhibiting lanosterol demethylase (Erg11p). The resulting depletion of cellular ergosterol and the accumulation of ‘toxic’ sterol intermediates are both thought …
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Characterization and sequence variation of the virulence-associated proteins of different tissue culture isolates of African Horsesickness virus serotype 4
… Vero cells and investigate their cytotoxic and membrane permeabilization properties within these cells. The NS3 and VP5 genes of AHSV-4(1) and AHSV-4(13) were successfully inserted into a mammalian expression vector and transiently expressed in Vero cells. The transfection procedure was …
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