Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"membrane dynamics"”.
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Advanced Fluorescence Correlation Techniques to Study Membrane Dynamics
… or binding parameters, both in solution and in membranes. However, it can suffer from severe artifacts, especially in non-ideal systems. Here we develop several novel implementations of FCS which overcome these limitations and facilitate accurate and quantitative determination of dynamic …
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Probing cell membrane dynamics using plasmon coupling microscopy
The plasma membrane of mammalian cells is depicted as a two-dimensional hybrid material which is compartmentalized into submicron-sized domains. These membrane domains play a pivotal role in cellular signaling processes due to selective recruitment of specific cell surface receptors. The structural …
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RABPHILIN3A: FROM NMDA RECEPTOR MEMBRANE DYNAMICS TO NEURODEVELOPMENTAL DISORDERS
… impairs GluN2A accumulation at postsynaptic membrane, preventing LTP induction. Our group has also previously demonstrated that overexpression of Rph3A in primary hippocampal neurons is sufficient to occlude the LTP-induced formation of new dendritic spines, confirming a key role for the …
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Quantifying tympanic membrane dynamics in otitis media using low coherence interferometry
Examining middle ear dynamics and mobility of the tympanic membrane (TM) is essential when diagnosing otitis media (OM), or a middle ear infection. OM is a widespread infectious disease in childhood, characterized by the accumulation of middle ear fluid, known as middle ear effusion (MEE). Loss of …
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… of cholera toxin B-labeled intracellular membranes to the plasma membrane. Cholera toxin-B labels the ganglioside GM1, which is enriched in lipid rafts. These specialized membrane domains are thought to serve as signaling hubs bearing many proteins that in turn, mediate trafficking steps …
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Investigating the C-terminal region of RalA: regulation through membrane dynamics and protein-protein interactions
… between RalA and RalB, but also serves as a membrane anchor via a polybasic region and prenylation and has been suggested to play key roles in modulation of the function of these proteins. A more robust understanding of this hypervariable region and its function is required before attempting …
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Computational and Experimental Investigation of the Critical Behavior Observed in Cell Signaling Related to Electrically Perturbed Lipid Systems
… directly impact the intracellular mitochondrial membrane at clinically relevant field amplitudes. As demonstrated in this work, PEFs may influence the mitochondria via an indirect route such as disruption of the actin cytoskeleton and/or alteration of ionic environment in the cytoplasm due to …
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BIOCHEMICAL CHARACTERIZATION OF ETHANOL MODIFIED PHOSPHOLIPIDS IN SACCHAROMYCES CEREVISIAE
… IS REGARDED AS AN ABNORMAL LIPID IN CELLULAR MEMBRANE, UNIQUELY FORMED FROM PHOSPHATIDYLCHOLINE VIA PHOSPHOLIPASE D-CATALYZED TRANSPHOSPHATIDYLATION IN THE PRESENCE OF ETHANOL. IT HAS A DISTINCTLY DIFFERENT CHEMICAL STRUCTURE COMPARED WITH OTHER PHOSPHOLIPIDS AND MAY PERTURB THE …
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Characterization and transcriptomic profiling of Arabidopsis thaliana under cellulose biosynthesis inhibitor treatment
… models and predicted a protein with seven-membrane spanning helices. Transcriptomic profiling of Arabidopsis thaliana in response to CBI treatment revealed differential expression of genes related to membrane dynamics, stress responses, cellular transporters, and general metabolism. …
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Investigation of Phosphatidylinositols and Phosphoinositides Using Matrix- Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
… in situ, making it invaluable for studying lipid dynamics in health and disease. This thesis focuses on phosphatidylinositols (PIs) and their phosphorylated forms, phosphoinositides (PIPs), which are critical regulators of cellular signaling, membrane dynamics, and cytoskeletal organization. …
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The impact of microgravity conditions on human gut microbiome
… conditions. Lipids play a crucial role in membrane integrity, signaling pathways, and cellular metabolism, and their alterations may reflect adaptive responses to gravitational changes. By employing advanced lipidomics and metabolomics approaches, this study identifies significant changes …
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Role of Bacterial Effectors SopD and SopB in Pathogenicity of Salmonella enterica serovar Typhimurium.
… remained elusive. Here I show that SopD affects membrane dynamics during S. Typhimurium invasion of epithelial cells. SopD promotes membrane sealing and macropinosome formation, events that may have important consequences for efficiency of bacterial cell entry in vivo. Furthermore, we demonstrate …
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New optical techniques and hardware for studying live cell dynamics
… The technique was used to image red blood cell membrane dynamics in real time with no increase in photobleaching or toxicity rates compared to standard widefield imaging. This work also presents 3D computational reconstructions of the data allowing for easier visualisation and the possibility of …
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Characterization of a Chlamydomonas protein involved in cell division and autophagy
… related to basic cellular processes such as membrane dynamics and vesicle budding and fusion; several physiological processes in animals, such as synaptic neurotransmission and blood filtration in the kidney, are related to the CV's function; and several pathogens, such as the causative …
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Reorganization of mitochondrial architecture during apoptosis and mitophagy as investigated by correlative microscopy
… effort to better understand the contribution of membrane remodeling to these biological processes. During apoptosis, Bax/Bak oligomerize and accumulate in and adjacent to the outer mitochondrial membrane, resulting in outer membrane ruptures several hundred nanometers wide. Using correlative …
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Engineering Substrates for the Study of Cell Mechanical Interactions
… substrates to help us understand plasma membrane dynamics. The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and …
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Investigating the Molecular Mechanisms of TMEM16F – a Ca2+ Activated Phospholipid Scramblase and Ion Channel
<p>Transmembrane protein 16 (TMEM16) is a novel family of transmembrane proteins that function either as ion channels, lipid scramblases or both. In mammals, the majority of TMEM16 members are Ca2+-dependent phospholipid scramblases (CaPLSases) that catalyze bidirectional movement of phospholipids …
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A Computational Study of The Electrical Response of Biological Cells with Realistic Three-Dimensional Morphologies
… unique features of a biological cell is the cell membrane that protects the cell interior by establishing a physical barrier between the cytoplasm and the extracellular matrix and governs the distribution of the cytoskeleton to control the three-dimensional (3D) morphology of a cell. From an …
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Multi-Scale Modeling for Analysis and Design of Transcranial Electric and Magnetic Brain Stimulation
… to neuron models incorporating non-linear membrane dynamics and realistic morphologies for predicting the neural response to TMS and tES. Indeed, extrapolating the neural response (polarization or threshold) from the uniform E-field or macroscopic E-field components often led to erroneous …
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Efficient coarse-grained brownian dynamics simulations for dna and lipid bilayer membrane with hydrodynamic interactions
The coarse-grained molecular dynamics (CGMD) or Brownian dynamics (BD) simulation is a particle-based approach that has been applied to a wide range of biological problems that involve interactions with surrounding fluid molecules or the so-called hydrodynamic interactions (HIs). From simple …
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