Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"membrane domains"”.
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Characterization of erythrocyte membrane domains using fluorescence digital imaging microscopy
… components of fluorescently labeled erythrocyte membranes. Experiments with erythrocytes and erythrocyte ghosts labeled with fluorescent phospholipids showed that the lipids are distributed laterally into areas of enrichment, or domains. Double-labeling experiments of ghosts using NBD- and …
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Lateral Membrane Domains Organize and Regulate Components of Signal Transduction Pathways
… (MAPK) signaling cascade also localized into domains in vesicles containing acidic lipids. The formation of lateral membrane domains induced by Ki-Ras 4B and the Raf-cysteine rich domain (Raf-CRD) was inhibited by the anti-tumor agent farnesylthiosalicylic acid (FTS). The inhibition of Ras …
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Formation of membrane domains during the activation of protein kinase C
The relationship between lipid domains and protein kinase C activity was studied via the direct visualization and quantitation of domains by fluorescence digital imaging microscopy. The lateral organization of protein kinase C, its substrate (the MARCKS peptide) and its three activators …
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The Role of Membrane Domains In Protein and Lipid Sorting During Endocytic Traffic
… lipid and protein composition of the plasma membrane (PM) must be tightly controlled to maintain cellular functionality, despite constant, rapid endocytosis. Because <em>de novo </em>synthesis of proteins and lipids is energetically costly, the cell depends on active recycling to return …
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Formation of membrane domains induced by the G and M proteins of vesicular stomatitis virus
… virus (VSV) buds from specialized regions or domains on the plasma membrane of infected cells. These domains are composed mainly of viral proteins and they are enriched in certain lipid species. The properties of the two envelope-associated proteins of VSV, the glycoprotein (G) and the matrix …
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Probing cell membrane dynamics using plasmon coupling microscopy
The plasma membrane of mammalian cells is depicted as a two-dimensional hybrid material which is compartmentalized into submicron-sized domains. These membrane domains play a pivotal role in cellular signaling processes due to selective recruitment of specific cell surface receptors. The structural …
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Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled …
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IQGAP1 is a novel effector of gonadotropin-releasing hormone receptor signaling
… that GnRH receptors reside in low-density plasma membrane domains where they participate in multiprotein signaling complexes. Here we used quantitative proteomics to identify proteins associated with low-density plasma membrane domains and to measure changes in their relative abundance in these …
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Lysosome biogenesis during osteoclastogenesis
… well studied, and the nature of their specific membrane domains is now established. Sensing the proximity of a bone-like surface the cell reorganises its cytoskeleton, and creates specific membrane domains: an actin-rich ring-like zone (named actin ring) surrounded by highly ruffled membrane …
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The Role of Sulfatide in Alzheimer's Disease
… enzymes which are preferentially sequestered to membrane domains called lipid rafts. Sulfatide (ST) is a glycosphingolipid that is essential for the proper structure and function of lipid rafts. In mice that lack ST, membrane domains that are normally maintained by adhesive contacts and …
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Organization and formation of the apical membrane of epithelial cells
Compartmentalization of cell membranes, in particular of the apical membrane of columnar epithelia, is the topic of this thesis. The first part characterizes the apical membrane and its specialized organization and morphology, whereas the second part focuses on the formation of this unique plasma …
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Influence of protein and lipid domains on the structure, fluidity and phase behavior of lipid bilayer membranes
… bilayer forms the basic structure of the cell membrane, which is a heterogeneous matrix of proteins and lipids that provides a barrier between the interior of a cell and its outside environment. Protein and lipid domains in cell membranes can facilitate receptor localization, stabilize …
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Dissection of CD2AP functions in epithelial cells
… by extensive cell-cell contacts along lateral membranes that hold the cells together in cohesive sheets and by a pronounced apical-basal polarity in which the apical domain faces a lumen and the basal domain contacts the basement membrane of the extracellular matrix. Therefore, all epithelial …
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Biophysical and biochemical properties of the mammalian male germ cell specific sulfogalactosylglycerolipid (SGG): Contribution to the structure and zona pellucida affinity of pig sperm raft membranes
Raft membranes are implicated in cell adhesion. Here, we demonstrated that rafts isolated from hypermotile capacitated pig sperm as low-density Triton X-100 insoluble membranes had the ability to bind specifically to homologous zonae pellucidae (ZP). This binding was dependent on pig ZP3alpha …
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Localization of Human Prostaglandin E2 Receptors in Polarized Epithelial Cells
… understood. Several studies have determined membrane targeting of G protein-coupled receptors (GPCRs) using epithelial cells such as Madin-Darby canine kidney (MDCK) cells. Polarized epithelial cells are composed of apical and basolateral plasma membrane domains with specific protein …
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Membrane-cytoskeleton associations during myogenesis
… muscle. To examine this possibility, plasma membrane-cytoskeleton associations involving specific membrane proteins were studied in developing L8E63 cells on the basis of the following criteria: Triton-insolubility, co-localization and co-extraction with cytoskeletal proteins, and sensitivity …
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Altered Cholesterol Metabolism In Human Cancers Unraveled By Label-Free Spectroscopic Imaging
… thesis work also found that cholesterol-rich domains on plasma membranes can be used as a marker for the loss of basoapical polarity, one of the earliest changes observed in breast neoplasia. Raman microspectroscopy revealed that in polarized acini lipids were more ordered at the apical …
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Modeling mammary epithelial cell polarization and the role of podocalyxin in breast tumor progression
… spheroids embedded in a reconstituted basement membrane. I first used this assay to characterize the role of cell-cell and cell-ECM adhesion in the formation and polarization of the apical junction complexes in normal mammary epithelial cells. Cell-cell adhesion alone was sufficient to initiate …
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Lipid rafts in protein sorting and yeast cell polarity
… are targeted via different pathways to distinct membrane compartments of the cell. The molecular principles that operate in cargo sorting at the TGN are still not very well understood. Especially, we know very little about the sorting of lipids. It was postulated that a sorting mechanism based on …
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Exploring the roles of key lipids in the influenza virus lifecycle
… and sphingolipids form distinct plasma membrane domains, termed lipid rafts, that help recruit newly synthesized influenza components to virus assembly sites for progeny budding. However, the sphingolipid and cholesterol abundance at this bud zone remains a mystery due to the limited …
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