Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"membrane disruption"”.
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Mechanisms of Adenovirus Membrane Permeabilization
… to occur, a virus must first penetrate host cell membranes to access intracellular sites of viral replication. Currently the mechanism through which adenovirus, a non-enveloped, dsDNA virus, disrupts the endosomal membrane during cell entry is not well characterized.</p><p>Recent studies suggest …
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Synthesis and dye leakage assay of human and rat islet amyloid polypeptide 10-19 and insulin 14-18
… The ability of hIAPP 10-19 to disrupt membranes was tested using a dye leakage assay. The fluorescence of the peptide was measured in increasing concentrations to determine whether their ability to cause cell death is concentration dependent or not. While the rate of membrane disruption …
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… to create pores along the bacterial cell membrane. Current methods for assessing pore formation do not directly measure cell permeabilization or enable same-day assessment. In this thesis, a novel fast-screening protocol combining SYTOX green, microfluidics, and fluorescence imaging is …
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Biosynthesis and engineering of lanthipeptide natural products for novel applications
… II-α-β complex induces pore formation on the membranes. In this chapter, lipid II analogues were synthesized that demonstrated the Halα-lipid II interaction. Interestingly, leakage assay on model membranes suggested that lipid II is not required for the membrane disruption activity, and the …
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Inactivation of foodborne pathogens by fruit wines
… in cell size, holes in the cell wall and membrane disruption that appeared to have caused leakage of intracellular contents.
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Search For The Functions That Mediate Protein Palmitoylation And Depalmitoylation
… the <i>trans</i>-Golgi network to the plasma membrane. Bch2 was the strongest candidate from screens of two different overexpression libraries and <i>BCH2</i> overexpression decreases the amount of palmitoylated Yck2 in palmitoylation assays. Results presented here show that <i>BCH2</i> …
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Structural Influences on the Photochemistry and Photophysical Properties of p-Phenylene Ethynylenes: Aggregation Effects and Solvent Interactions
… in two systems: surfactants and model bacterial membranes. The placement of the ionic alkyl substituents played a dominant role in determining the outcome of molecular interactions and type of aggregates which resulted between OPEs and both systems. Biophysical simulations of the interactions …
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Calcium -dependent protein kinases are myristoylated and associated with different membranes in Arabidopsis thaliana
… in many locations in plant cells, including the membrane fraction. However, little is known about the subcellular locations of individual CDPKs or the mechanisms involved in targeting them to those locations. Arabidopsis contains 34 genes that are predicted to encode CDPKs and 28 of the predicted …
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Quantification of membrane transport rates using optofluidics
The transport of ions and small molecules across membranes is a ubiquitous process in biological systems. Effective studies of membrane transport phenomena require suitable model membrane systems, efficient methods to quantify transport kinetics and a robust theoretical framework to derive …
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Midaesin - a histone H2A antimicrobial peptide from the South African abalone, Haliotis midae
… and fluorescent microscopy, employing the membrane impermeable dye propidium iodide, revealed membrane disruption of V. anguillarum cells exposed to 13 µM or 30 µM of the Midaesin-containing peptides for 30 minutes, respectively. Secondary structure analysis by circular dichroism indicated …
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Influence of Membrane-Damaging Agents and the Sigma Factor AlgU on the Induction of the MexCD-OprJ Efflux System of Pseudomonas Aeruginosa
… normal laboratory conditions, exposure to the membrane-active biocides, chlorhexidine or benzalkonium chloride, results in mexCD-oprJ expression. This suggests that membrane disruption provides the inducing signal. Consistent with this, increased mexCD-oprJ expression was demonstrated in the …
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A Study of The Effect of Membrane Targeting Antimicrobial Compounds on Ionic Transport Across Lipid Bilayer Membranes
… properties through studying their peptide-membrane interactions. Melimine has previously been shown to reduce the integrity of membranes, observed through the leakage of dye from bacterial membranes. The membrane-peptide interactions were compared using advanced lipid membrane biophysical …
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Immunolocalization of dystrophin and neurofilament protein in muscle spindles of normal, mdx-dystrophic, and denervated mice
… not an integral part of the subsynaptic sensory-membrane in equatorial regions of normal intrafusal fibers, and, that the neurotrophic effect of sensory innervation is not the principle cause of this unique arrangement of dystrophin in equatorial regions. In dystrophy, intrafusal fibers display …
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Single-molecule studies of alpha-synuclein and abeta in Parkinson’s disease derived biofluids
… biological assays by quantifying the extent of membrane disruption and consequent Ca2+ -influx into single-liposomes and the production of a proinflammatory cytokine (tumour necrosis factor alpha) by microglia-like cells. This result supports the hypothesis that smaller protein aggregates are …
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Advances in understanding protein-lipid interactions through biophysical and bioelectronic approaches
… Lipids form the majority component of the cell membrane. They are not just involved in maintaining the structural integrity of the plasma membrane, but are also involved in more complex processes such as signalling, regulation and intracellular transport. As the IDPs mentioned above are known to …
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Investigation and application of heterochiral proteins enabled by flow-based peptide synthesis
… kill the pathogen through a mechanism of membrane disruption. Our lead heterochiral ADC candidate is being advanced through additional pre-clinical studies.
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Non-Invasive Eradication of Biofilm on Metal Implants Using Alternating Magnetic Fields (AMF) and Antibiotics
… intervals. Initial mechanistic studies revealed membrane disruption as one factor important for AMF enhanced antibacterial activity in the biofilm. Then, the impact factors generated by AMF, heat and electric current, to metal implants were studied separately. A mathematic model was built to …
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The discovery and functional assessment of novel peptides from the skin secretions of frogs
… and the human cancer cells through cell membrane disruption and they exhibited weak haemolytic activity towards horse erythrocytes at their effective concentrations. The modified analogue significantly increased the antimicrobial and anticancer potency of QUB2605. Compared with the human …
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Metabolic engineering of microorganisms for the overproduction of fatty acids
… analysis revealed acid stress response, membrane disruption, colanic acid and biofilm formation during fatty acid production, thus pinpointing the targets for future metabolic engineering effort. These results highlight the benefit of using computational strain design and system metabolic …
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