Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"membrane attack complex"”.
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Components of the Membrane Attack Complex (Mac) of Human Complement: Functional Studies of Their Macpf Domains
… assembly of C5b, C6, C7, C8, and C9 to form the membrane attack complex (MAC). C6, C7, C8α, C8β, and C9 make up the “MAC family” of proteins. MAC family proteins have homologous N and C terminal modules and a central MACPF domain named for sequence similarity between the MAC family of proteins …
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Human Complement Protein C8: Crystal Structure and Analysis of Binding Interactions
Human C8 is part of the membrane attack complex (MAC) that assembles on bacterial cell membranes to form a lethal pore-like structure. The MAC is comprised of complement proteins C5b, C6, C7, C8 and C9. The 150-kDa C8 protein consists of three non-identical subunits (C8alpha, C8beta, C8gamma), …
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Molecular Interactions of Human C9: The Pore Forming Protein of the Complement System
… active pore-like structure known as the membrane attack complex (MAC). C8 is a heterotrimer comprised of a disulfide linked C8α-γ dimer that is non-covalently associated with C8β. Following assembly of C5b-8, C8α-γ facilitates the binding and polymerization of C9. C9 along with C6, C7, …
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The Colonization Strategies of Nontypeable Haemophilus influenzae - Bacterial Colonization Factors and Vaccine Development
… This interaction delayed the formation of the membrane attack complex on the bacterial surface and increased the bacterial serum-resistance. Furthermore, we identified a novel surface-exposed virulence factor designated Haemophilus Protein F that also sequesters vitronectin on the surface of …
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Investigating NLR-mediated immunity and Pseudomonas syringae effector interactions with two MACPF domain proteins in Arabidopsis thaliana.
… of monitored proteins are detected. MEMBRANE ATTACK COMPLEX/PERFORIN (MACPF) proteins are well known agents of defense in the mammalian immune system, though the exact role of this protein family in plants has not been established. Mutations in two of the four MACPF domain proteins …
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Haemophilus influenzae adhesins and the innate immune response
… elderly. H. influenzae expresses several outer membrane proteins of which the adhesins play an important role in the initial interactions with the airway epithelium. In this thesis, we have studied two Haemophilus influenzae adhesins and their interactions with innate defense mechanisms. …
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Complement in Disease - Extracellular Proteins as Complement Regulators
… C9 and inhibiting the formation of the lytic membrane attack complex. PRELP was also found to inhibit the assembly of the alternative pathway C3-convertase and thereby affects two stages of the complement cascade. We found that cartilage oligomeric matrix protein (COMP), which has been shown …
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Microglia and Complement in Alzheimer's Disease with Cerebral Amyloid Angiopathy
… We have found increased levels of the lytic Membrane Attack Complex (MAC) on vessels harboring CAA compared to vessels from AD patients without CAA and pathological controls. Additionally, the same vessels also show trending decreases in the endogenous MAC inhibitor, CD59. We believe the …
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The role of a pore-forming protein, Mpeg1, in cytosolic import
… import in DCs. Mpeg1 is a member of the membrane attack complex/perforin (MACPF) superfamily. We find that DCs deficient in Mpeg1 are less efficient in their ability to import saporin into the cytosol, and that the activity of Mpeg1 is regulated by low pH and potentially proteolytic …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… Perforin-2 is a member of the membrane attack complex and perforin superfamily (MACPF) and, similar to perforin and complement, can form oligomeric pores on liposomes *in vitro*. However, the regulation of perforin-2 pore formation in the context of endocytic escape *in vivo* is …
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The capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5A: role in serum resistance and characterization of the genetic basis for expression
… by limiting the amount of C9, a component of the membrane attack complex, but not C3, that bound to the bacteria in PCS. A second mechanism of serum resistance was due to a lipopolysaccharide (LPS)-specific antibody present in the IgG fractions of normal swine serum, swine anti-K17 serum, and …
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Anti-GD1a antibody targeted disruption of the node of Ranvier in a mouse model of acute motor axonal neuropathy
… It is here that susceptibility to antibody attack in a mouse model of GBS has previously been demonstrated. It was the aim of this thesis to determine to what extent more proximal portions of the distal axon are at risk from circulating antibody and what exogenous protection can be provided …