Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 78 for “"melanocyte"”.
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Pleiotrophin inhibits melanogenesis in normal human melanocyte
… Little is known about the effects of PTN on the melanocyte function and skin pigmentation. In this study, we investigated whether PTN would affect melanogenesis. PTN was expressed in melanocytes and fibroblasts of human skin. Transfection studies revealed that PTN decreased melanogenesis, …
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Melanocyte survival and response to treatment in Vitiligo Patients
… Although evidence strongly suggests that melanocytes die in various forms of vitiligo, it is also evident, at least in certain cases, that melanocytes/melanoblasts survive in an undifferentiated stem cell form and it is from these that re-pigmentation is assumed to occur. Treatment of this …
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The Establishment and Regulation of Melanocyte Stem Cells in Zebrafish
… and regulation of stem cells during zebrafish melanocyte regeneration.</p><p>In the first part of this thesis, I develop a drug based method of ablating melanocytes in the adult zebrafish body. The drug is a copper chelator, neocuproine: NCP) that I show causes death specifically of melanocytes …
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The role of alpha-melanocyte stimulating hormone in environmentally induced color change.
… is due to pituitary secretion of alpha-melanocyte stimulating hormone (α-MSH). However, physiological levels of α-MSH has not been reported for several species that have historically been the models of choice for investigating changes in pigmentation. The goal of this research was to …
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Melanocyte proliferation in wounded skin in vitro: a histological and immunohistochemical analysis
… by the wound in an efficient and timeous manner. Melanocytes are not thought to actively participate in wound repair and closure, since they are primarily responsible for maintenance of normal skin pigmentation. Mechanisms of keratinocyte migration during the re-epithelialisation phase of …
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An ultrastructural and light microscopic study of melanocyte differentiation in chick embryos
… directing the in vivo differentiation of melanocytes from crest cells are as yet unknown. To begin to address this issue, it is important to establish exactly when and where these signa/s first exert their effects. Therefore, in the present study, overtly differentiated melanocytes …
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OXYGEN-17 NUCLEAR MAGNETIC RESONANCE OF GLYCINE, ALANINE, DIPEPTIDES AND MELANOCYTE INHIBITING FACTOR (NMR).
… RESONANCE OF GLYCINE, ALANINE, DIPEPTIDES AND MELANOCYTE INHIBITING FACTOR (NMR).
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Not Black and White: BMP Signaling Drives Melanocyte Differentiation Downstream of Stem Cell Activation
… mechanisms that fuel these transitions in melanocyte stem cells (McSCs), a unique neural crest-derived SC population located in the hair follicle (HF). Through periodic bouts synchronous with HF cycling, quiescent McSCs become activated to proliferate, giving rise to committed proliferative …
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Oncogenic mutation of GNAQ/11 disrupts melanocyte biology in a Zebrafish model of uveal melanoma
… in humans. These tumors arise from nonclassical melanocytes found in the choroid, iris, and ciliary body (collectively called the uvea). Over 80% of UM tumors contain activating mutations in one of two genes encoding the homologous G[alpha] proteins GNAQ or GNA 11, which are the [alpha subunit of …
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Human-Mouse Neural Crest Chimeras as a Novel Model for Human Melanocyte Biology in Development and Disease
… approaches which focus on recapitulating the melanocyte neural crest lineage to pave the wave for a novel immune-competent melanoma model: First, we try adapting human donor cells to the mouse host environment via expression of a single mouse receptor (c-Kit) on human donor cells to rescue an …
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Regulation of melanogenesis in conditionally immortalised mouse melanocytes expressing a temperature-sensitive SV40 large T antigen
The transformation of a normal melanocyte to a malignant melanoma involves a series of poorly understood genotypic and phenotypic alterations. In vitro models of melanoma formation generated by transforming mouse melanocytes with exogenous oncogenes have revealed that this process is frequently …
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The cellular basis of the Southern African forms of rufous & tyrosinase-positive oculocutaneous albinism
… investigations of both hairbulb and skin melanocytes from the tyrosinasepositive and rufous forms of oculocutaneous albinism to elucidate the cellular aetiology of these disorders. In addition, melanocyte numbers were quantitated in the tyrosinase-positive skin to establish whether the …
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Hypomelanosis in chickens
… as vitiligo and albinism. To determine whether melanocytes are present in the hypomelanotic skin, two melanocyte-specific assays were carried out, in situ DOPA histochemistry and a sensitive radiometric assay for tyrosinase. The results show that active tyrosinase was present in 8, 9 and 10 day …
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The use of in vitro 2d co-culture models to determine the optimal keratinocyte: melanocyte ratio to be used in the development of pigmented 3d skin model
… to achieve this. Amongst many, inclusion of melanocytes, other skin appendages as well as potential progenitor cells is some of the attributes of an ideal 3D skin equivalent. Therefore pigmented 3D skin constructs are of great interest as they address not only the issues of complete wound …
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The control of melanogenesis in early avian embryos
… and differentiation of neural crest cells into melanocytes, to discover where in the developing embryo these events take place and to investigate the mechanisms whereby these processes are controlled. Homo- and heterotypic combinations of white (Yhite Plymouth Rock x Cornish Game) and black …
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Development of inducible transgenic mouse models for melanoma
… promoter was only responsive to the inducer when melanocytes were proliferating. Initially, regulator vectors were created by sub-cloning Cre under the control of a melanocyte-specific promoter either enhanced tyrosinase (EICre) or tyrosinase related protein 2 (Trp2Cre). A Cre responsive, target …
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Receptor mediated targeting of liposomes
… the cells have been investigated using either α-melanocyte stimulating hormone or Ricin-B-chain as ligands for promoting the binding of liposomes to cells. α-melanocyte stimulating hormone has been conjugated to liposomes and to Ricin-A-chain via the Lys₁₁ residue without significant loss of …
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
… by ectopic expression. Then we took directed melanocyte differentiation as a model to enrich transgenic differentiating cells. For this, a bicistronic plasmid was constructed that enables the transgenic medaka embryonic stem cell to differentiate into melanocyte and confer drug resistance and …
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The Effects of PPAR-γ Activators on the Growth and Apoptosis of Melanocytes
… that 3 subtypes of PPAR are expressed in human melanocytes and activators for PPAR-α and PPAR-γ modulates melanocyte growth and melanogenesis. In this study, I investigated the effects of PPAR-γ activators on melanocyte growth and apoptosis. I further examined the regulation of extracellular …
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