Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"medium spiny neurons"”.
-
A flow cytometry-based method for gene expression profiling of CNS cell types
… expressed genes between two types of medium spiny neurons in the striatum with similar or higher sensitivity, using transgenic mice with fluorescence proteins labeling the desired cell types. Immunofluorescence experiments were also performed to label cell nuclei isolated from wild …
-
Deconstructing G Protein-Coupled Receptor Dimer Pharmacology: Case Studies in Dopamine D1 and D2 Receptors
… or higher order oligomers. Particularly in medium spiny neurons of the striatum, dopamine receptor subtypes are reported to form dimers with themselves or other receptors (e.g. adenosine receptor A2A). Although a functional relevance for these dimers has been proposed, current assay systems …
-
Role of Tyrosine Receptor Kinase B in the Development and Function of the Central Nervous System
… the survival of sympathetic and sensory neurons. In the CNS, however, BDNF-TrkB interaction has been shown to regulate diverse aspects of development, physiology and pathology. In the current studies we focus on the roles of TrkB and its downstream signaling pathways in the progression …
-
Cell-Type Specific Translational Profiling in Huntington's Disease Mouse Models
<p>Medium spiny neurons (MSNs) are among the most vulnerable cell populations in Huntington's Disease (HD). Within this population, striatopallidal MSNs are more vulnerable than striatonigral MSNs, which may explain the typical progression in HD of chorea to hypokinesis. The biological basis for …
-
Characterization of the Increased Risk of Attention Deficit Hyperactivity Disorder Following Early-Life Exposure to Deltamethrin
… in voltage-gated sodium (Nav) channel 1.6 and medium spiny neurons which highly express Nav1.6- both of which are key components of reward circuitry. These studies are further complemented by identification of behavioral aberrations that typify the human pathophysiology of ADHD. Together, this …
-
Neuroprotective effects of overexpression of the inhibitor of apoptosis proteins in the quinolinic acid model of excitotoxic injury.
… which results in the selective loss of the medium spiny neurons in the striatum. The neuropathological and behavioural deficits of HD can be modeled in rats by injection of the NMDA receptor agonist quinolinic acid (2,3 pyridine decarboxylate; QA) directly into the striatum. The first aim of …
-
Ethanol priming and its effects on consumption and accumbal plasticity
… (D1) or D2-dopamine receptor (D2) expressing medium spiny neurons (MSNs) that precipitate different intracellular cascades and actions upon the reward circuitry. Alterations in the expression N-methyl-D-aspartate receptor (NMDAR)-dependent long-term depression (LTD) in the D1 MSNs of the NAc …
-
Morphological characterization of medium spiny neuron development in vitro
Medium Spiny Neurons (MSNs) are the primary cell type of the striatum, a structure critically involved in motivation, memory, and movement. MSN structure and function are altered in Parkinson’s and Huntington’s disease as well as following repeated exposure to drugs of abuse. One site on the MSN …
-
Neural Stem Cells as a Model to Study Huntington’s Disease
… diminishes cognitive abilities, by affecting the medium spiny neurons in the brain. In HD patients, neurons are damaged and destroyed because of the toxicity of the mutant Huntington protein (mHtt). The mechanism of how mHtt protein affects the neurons is unknown. In this study we explored the …
-
Flow cytometry quantitation of dopamine receptor D2 loss as a sensitive measure of Huntington's Disease progression in mouse neurons
… for the isolation and characterization of adult neurons by flow cytometry. Drd2 is a well-studied marker of a particularly vulnerable population in HD patients, the indirect medium spiny neurons of the striatum. Using this technique, I have demonstrated the ability to accurately and rapidly …
-
Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… neuronal excitability in nucleus accumbens medium spiny neurons. The new knowledge gained from this study might be useful for the treatment of Nav1.6 channel-related brain disorders such as epilepsy, schizophrenia and cognitive disorders.
-
Pharmacological inhibition of CK2: the effect of an FDA designated orphan drug on pathology, neuroinflammation, and glial phenotypes in Huntington’s disease
… mHtt aggregation, decreased the number of medium spiny neurons (MSNs) and allowed increased infiltration of mHtt aggregates into white matter fascicles. Removal of GFAP+ astrocytes also increased the number of white matter fascicles in the DMS. Overall, these results support a …
-
Dopamine-dependent Transcriptional Dynamics in Striatal Physiology and Cocaine Reward
… and transcriptional responses of NAc medium spiny neurons (MSNs) following acute cocaine experience. Acute cocaine exposure produced rapid but prolonged increases in neuronal activity of a subpopulation of neurons in the NAc. Using a real-time place conditioning behavioral assay in …
-
Pathobiology of neuroinflammation and basal ganglia circuitry in Parkinson’s Disease
… of PD is the progressive death of dopaminergic neurons in a basal ganglia nucleus termed the substantia nigra (SN). Another hallmark feature of PD pathology is the presence of Lewy bodies and Lewy neurites, which are cellular inclusions with aggregated protein depositions, representing pathology …
-
Modelling Huntington’s Disease Using Direct Cell Reprogramming
… in the preferential degeneration of striatal medium spiny neurons (MSNs) is limited. This may be due to the challenge of generating a representative model of HD. Cell reprogramming is a revolutionary technology that can generate live, human neurons in vitro from the somatic cells of …
-
The Role of Cdk5 in the Regulation of Dopamine Neurotransmission, Neuronal Excitability, and Reward-Related Behavior
… were accompanied by increased excitability of medium spiny neurons in the nucleus accumbens (NAc) in conditional Cdk5 knock-out mice. Targeted deletion of Cdk5 in the NAc facilitated cocaine-induced locomotor sensitization and conditioned place preference for cocaine. These results suggest that …
-
Modulation of Signal Transduction in the Nervous System by Protein Phosphorylation
… may regulate neuronal signal transduction. Medium spiny neurons, the principal cell type of the neostriatum, contain D1 and D2 dopamine receptors. D2 receptor activation is coupled in dissociated neurons to an outward, voltage-dependent, transient K<sup>+</sup>conductance, probably …
-
Hybrid Brain-Tissue Based Model to Study the Role of Infiltrating Monocytes in Late Stage Huntington's Disease
… is characterized by selective loss of medium spiny neurons (MSNs) in the striatum and cortical neurons in the cortex. It is caused by an expansion of CAG repeats in the exon-1 of IT-15 gene, which results in a mutated polyglutamine domain in the huntingtin protein (HTT) (Collaborative, …
-
Presynaptic signalling in Huntington’s disease
… and causes neurodegeneration, principally in the medium spiny neurons of the striatum. The symptoms of HD include loss of motor coordination (chorea) and cognitive deficits such as loss of memory and concentration. Currently, there are no treatments available that can cure or prevent HD. More …
-
Investigating three-dimensional (3D) gelatin methacryloyl (GelMA) hydrogel as a potential scaffold for cell transplantation in Huntington's disease
… pathway to treat HD by replacing the striatal neurons lost during the disease progression with healthy ones. The emergence of cell reprogramming technology offers a powerful tool to generate an expandable cell source that can differentiate striatal-specific precursor cells. Human embryonic stem …
Page 1 of 2