Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"media formulation"”.
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A Physical, Cultural, and Nutritional Study of "Leptospira Interrogans" Serotype "Hardjo" with Emphasis on Antigenicity, Immunogenicity, and Viability
… strain of "L. hardjo" were grown in different formulations of bovine albumin polysorbate media (BA-P80). Single and multiple deletions and different sources of ingredients for the medium were evaluated. Continuous growth was measured over a predetermined range of temperatures. The antigenicity …
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Positioning Microtissues on the Differentiation Continuum Reveals their Utility for Studying Skeletal Myopathies and Metabolic Disease
… the need for an insulin-free differentiation media formulation to enable T2D modeling. In parallel, we benchmarked the functional and metabolic properties of microtissues relative to 2D myotubes and native muscle. These findings identify a hurdle for the development of a multiplex …
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Enhancing Mesenchymal Stem Cell Chondrogenesis for Cartilage Tissue Engineering
… the effects of cell seeding density, media formulation and mechanical stimulation were examined with respect to functional growth. Transient application of TGF-β3 improved the compressive properties of MSC-laden constructs, but only when constructs were formed at a higher seeding …
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Feedbacks between flow, vegetation, deposition, and the implications for landscape development
… The initial flow field was solved using a porous media formulation for hydraulic resistance, and the velocity in wake regions was adjusted to match the wake structure measured in the laboratory study. Vegetation growth was added based on a probabilistic growth function linked to the velocity …
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The Influence of Necrotic Enteritis, Environmental Factors, and Genetics on Intestinal Development Pathways and Disease Occurrence in Broiler Chickens
Intensified poultry production to meet global food demands has faced challenges associated with the removal of in-feed antibiotics due to concerns over antibiotics resistance. The reduction of low-dose antibiotics in feed has allowed for reemergence of intestinal diseases that diminish animal …
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A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli
… such as plasmid design, strain variation, media formulation, and expression conditions. Protein expression at relatively high medium pH (pH = 9) led to a 4.47-fold and 5.46-fold improvement in soluble yield for two alcohol dehydrogenase models. The experimental design was further developed …
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Systems metabolic engineering through application of genome-scale metabolic flux modeling
… metabolic engineering problem) and culture media formulation design to achieve the solution. Results showed that (i) L-valine improves isobutanol production by Bacillus subtilis, (ii) cellobiose increases ethanol selectivity by Clostridium acetobutylicum ATCC 824, and (iii) B. subtilis is an …
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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis
… acid constitutes a substantial portion of media formulation costs in the proposed whole-cell process, and these findings allow for more efficient application of this and other precursor chemicals. Based on an analysis of the literature, it was hypothesised that oxygen was unlikely to be a …