Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"medaka"”.
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Cell behaviour during optic vesicle morphogenesis in medaka
… I therefore used an in vivo imaging approach in medaka fish to investigate the cellular movements and dynamics of early eye formation. Early medaka embryos were not amenable to in vivo imaging due to contractile movements that cause them to rotate in the chorion or in the embedding medium. I …
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Medaka fish as a model for complex trait genetics
In this thesis, I use the medaka fish (Oryzias latipes) and the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel — a genetic resource established by my supervisor, Prof. Ewan Birney, and collaborators — to investigate the genetic architecture of complex traits, with a particular focus on non-additive …
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A genetic analysis of somitogenesis in the Medaka (Oryzias latipes)
… species, a large scale mutagenesis screen in the medaka (Oryzias latipes) has been performed successfully in Kyoto, Japan. I analyzed nine of the isolated medaka mutants that show variable aspects of somitic phenotypes. This includes a complete or partial loss of somite boundaries (e.g. bms and …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… was to improve the generation of transgenic medaka fish. General transgenesis including transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first time efficient …
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Hypoxic Stress Induced Gene Modulation in Medaka (Oryzias latipes) and Xiphophorus maculatus
… (> 5.0 fold) changes in mRNA abundance when medaka were exposed hypoxic conditions. Herein we present results of the qRT-PCR studies to assess their potential utility as hypoxia biomarkers.
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The Influence of Estrogen Signaling on Male Reproduction in Medaka (Oryzias latipes)
… changes in the small aquarium model fish, medaka, by investigating effects of a general estrogen receptor agonist, ethinylestradiol (EE2), and those of a G-protein estrogen receptor (GPER) specific agonist, G-1. This was assessed through breeding experiments, histological assessment of …
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
The combination of medaka embryonic stem cell and bicistronic plasmid system make it a good platform to study various molecular and cellular events. We first developed a single plasmid system for fast assay of relative cellular activities for homologous recombination versus random integration. In …
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Japanese courage: a genetic analysis of complex traits in medaka fish and humans
… differences in behaviour, using the Japanese medaka fish as a model organism. Chapter 1 sets out the introduction to the diverse topics covered in this thesis, and is followed by five substantive chapters. Chapter 2 describes several genomic characteristics of the Medaka Inbred …
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Examining swim bladder inflation failure as a toxicological endpoint in Japanese medaka (Oryzias latipes)
… impaired swim bladder inflation of Japanese medaka (Oryzias latipes). After exposure to individual compounds, mixture exposures to no-observed-effect concentrations of these compounds were conducted. Significant effects on swim bladder inflation were observed for a binary mixture of …
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Generation of a Medaka Fish Model of Propionic Acidemia for Development of Novel Therapies.
… I have generated a PA model in medaka fishes (<i>Oryzias latipes</i>) using TALENs targeting the <i>pccb</i> medaka gene, encoding one of the subunits of PCC. The <i>pccb<sup>del/del</sup></i> medaka recapitulates the clinical and biochemical phenotype of human patients. Affected …
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Cloning of medaka glial cell-derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
… and characterize both Gdnf and GfrI?1 from the medaka fish (Oryzias latipes). A full length cDNA for the medaka gfrI?1 was obtained by RT-PCR followed by RACE. It is 2551 bp long and has an open reading frame of 1401 bp for 466 amino acids. The predicted protein shares the best homology to the …
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The Effects of Toluene on the Ontogenetic Development of the Fish, Oryzias latipes, the Japanese Medaka
… fish, Oryzias latipes, the Japanese Medaka was established. By the use of static bioassays, the median tolerance limits (TL 50 values) were calculated with their respective 95% confidence limits for different stages of embryonic development exposed to the chemical for periods of …
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Vitellogenin induction in male Japanese medaka exposed to the chemotherapeutics: oxytetracycline, Romet-30, and copper sulfate
The specificity of a medaka vitellogenin (Vtg) enzyme-linked immunosorbent assay (ELISA) kit was tested on adult hybrid tilapia to determine if vitellogenin could be successfully measured in tilapia using this method. Adult hybrid (Orechromis niloticus x Oreochromis mossambicus x Oreochromis …
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Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation
In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and …
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Perspectives On Ethanol Toxicity In Medaka Embryogenesis And Possible Protection By Asian Ginseng (Panax Ginseng): Histological And Biochemical Analysis
… especially zebrafish (Danio rerio) and Japanese medaka ( Oryzias latipes), are long-established models for research in developmental biology and have been used to explore alcohol's effect on neurogenesis, cardiogenesis, intracellular signaling, neurobehavioral aspects, and apoptosis. These two …
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Development and Standardization of a Short-Term Assay for Evaluating Polluted Estuarine and Coastal Environments: The Medaka Embryo-Larval Assay
<p>The eggs of the Japanese medaka (Oryzias latipes) were employed in a number of studies to develop a routine, standardized assay which can assess the acute and sublethal impacts of individual toxicants and complex mixtures. The eggs of this Cyprinodont minnow were topically treated with each …
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Aggression, monopolization and growth depensation within groups of Japanese medaka (Oryzias latipes) : interactions between the temporal and spatial clumping of food
… of food by allowing groups of ten Japanese medaka (Oryzias latipes) to compete, over a 4 week period, for food in a 2 x 2 factorial design. As expected, the intensity of aggression increased as the spatial clumping of food increased and as the temporal clumping of food decreased. However, …
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Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
… on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. These attributes include daily spawning, a number of useful …
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