Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"mechanosensation"”.
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Microtubules, Acetylation, and MEC-3 Regulated Genes in C. Elegans Mechanosensations
… to be a useful system in which to study neuronal mechanosensation and the development of specialized neuronal subtypes. Response to gentle body touch in C. elegans is mediated by the six Touch Receptor Neurons (TRNs). TRN cell fate is specified by the LIM-Homeodomain transcription factor MEC-3. …
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Molecular Basis of Mechanosensitivity
<p>Mechanosensation is arguably the least understood of all senses. For most physiological processes, the first response to membrane stress is thought to be the opening or closing of mechanosensitive channels, but the clonal nature of the first mechanotransducers is still largely unknown. The …
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Minimally invasive neuromodulation using mechanically-sensitive ion channels and magnetically-actuated nanotransducers
… describe the molecular biology behind conferring mechanosensation to neurons, the design of a membrane targeting mechanism via SNAPtags expressed on neuronal membranes, and the observed neuromodulatory effects for a gamut of mechanoreceptors and stimulation conditions. Calcium imaging results …
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Slow Touch and Fast Nociception: Exploration of the Interactions Between Noxious and Innocuous Mechanical Touch Sensation
… itch, and pain. When considering skin mechanosensation, the canonical view is that low force innocuous mechanical stimuli are encoded by cutaneous low threshold mechanosensitive receptor (LTMR) endings, innervated by fast conducting, thickly myelinated Aβ afferent nerves (Mountcastle, …
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Harnessing the Regenerative Characteristics of Meniscus Superficial Zone Cells
… profiling, migration, and proliferation assays, mechanosensation analysis, and traction force microscopy. After, the matrix deposition potential of superficial cells was assessed on electrospun nanofibers. Here, we show at the transcriptional level that superficial cells are most similar to those …
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Microfluidic-based Tools to Investigate Gene Expression and Regulation with Tissue-specificity during Aging in Caenorhabditis elegans
… underlying gene expressions in the context of mechanosensation. The completion of this thesis creates an array of new tools and frameworks that can improve the content of information we can gather on a single-animal level, enabling us to ask deeper questions in biology.
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Assessing the Role of the Transient Receptor Potential Melastatin 3 (TRPM3) Ion Channel in Visceral Sensation
… during colitis and selectively contributes to mechanosensation of wide-dynamic range colonic afferent nerve units during colitis. This study furthers our understanding of the mechanosensitive ion channels that contribute to visceral sensation and elucidates TRPM3 as a potential target for …
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Role of Transient Receptor Potential (TRP) Channels in Nociception
… Several receptors have been proposed to underlie mechanosensation. The molecular entities responsible for mechanosensation are not fully understood. In this study, I have characterized the properties of TRPV4, a putative mechanosensitive ion channel expressed in dorsal root ganglion (DRG) neurons …
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Development of computational and experimental tools to study mechanotransduction in C.elegans and primates
… an understanding of the processes leading to mechanosensation. More specifically we study and model (1) how surface loads are transmitted to embedded mechanoreceptors and (2) how one type of mechanoreceptor (slowly adapting type-i or SAl) transduce these mechanical signals to a sequence of …
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The Interplay Between Bone Homeostasis, Permeability, and Fracture Toughness
… enables nutrient delivery, cell signaling, and mechanosensation, which regulate bone remodeling and adaptation to mechanical demands. Bone’s ability to maintain structure, function, and quality depends on a delicate balance among mechanical forces, fluid flow, and cellular responses, all working …
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Mechanically driven In vivo Osteocyte Ca2+ Signaling: Aging and Pharmacologic Effects
… understanding of the extent of the decline of mechanosensation in aging bone. Furthermore, we have an idea of the respective contributions of numerous Ca2+ channels to osteocyte mechanotransduction in young, healthy bone of both male and female animals. Together, these advancements …
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Modulation of touch sensitivity in Caenorhabditis elegans
… is largely unknown. In this thesis, I study mechanosensation in the C. elegans touch receptor neurons (TRNs) to understand how sensory systems are modulated and adapt to the environment. I find that the touch sensitivity in the TRNs is modulated by both mechanical and non-mechanical factors. …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… of tissue development and homeostasis. Aberrant mechanosensation is associated with a wide range of pathologies, including diseases such as osteoarthritis (OA). Substrate stiffness is one of the well-known mechanical properties of the matrix that enabled establishing the central dogma of an …
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Application of Fluid Flow for Functional Tissue Engineering of Bone Marrow Stromal Cells
… loop to periodically halt flow, and markers of mechanosensation and osteoblastic differentiation were measured. These studies demonstrated that shear stresses of 0.36 to 2.7 dynes/cm2 stimulate late phenotypic markers of osteoblastic differentiation but not cell proliferation. In addition, this …
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High-throughput, single-worm tracking and analysis in Caenorhabditis elegans
… elegans experiments. Three genes, implicated in mechanosensation as a result of genetic sequence but lacking any observed phenotypic support, reveal locomotory phenotypes in our analysis. This prompts a large clustering of all 330 groups, to assess the predictive capabilities of our system. The …
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Molecular and Cellular Mechanisms of LIM Protein Mechanotransduction
… understanding the physiological significance of mechanosensation, the mechanisms by which proteins convert mechanical stimuli into biochemical signals (mechanotransduction) are poorly understood. The actin cytoskeleton serves as a nexus for cellular mechanotransduction, generating and transducing …
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The role of actomyosin contractility in zebrafish neural tube formation through hollowing.
… literature linking primary cilia function to the mechanosensation of fluid flow and regulation of barrier function in endothelial vessels, the role of primary cilia as mechanosensors has been controversial. Cilium-mediated mechanotransduction within the neural tube is so far unexplored and could …
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Funktionelle Interaktion von Polycystin 2 und TRPV4
Die autosomal dominante polyzystische Nierenerkrankung (ADPKD) ist eine der häufigsten monogenen Erberkrankungen weltweit. Sowohl Frauen, als auch Männer sind in einer Häufigkeit von 1:400 bis 1:1000 betroffen. Dabei führen Mutationen im PKD1- oder PKD2 Gen zur Bildung multipler Zysten in den …
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Mechanical Control of Sensory Hair-Bundle Function
<p>Hair bundles detect sound in the auditory system, head position and rotation in the vestibular system, and fluid flow in the lateral-line system. To do so, bundles respond to periodic, static, and hydrodynamic forces contingent upon the receptor organs in which they are situated. As the …
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Light Stimulation of Sensory Hair Cells
<p>Hair cells are the mechanosensory detectors that underlie our senses of audition and balance. Their mechanosensitivity arises from direct gating of force-sensitive ion channels by the tension in tip links located at the tops of the stereocilia. The study of hair cells and their mechanically …
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