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Showing 1 to 9 of 9 for “"maturase"”.
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Expression and Function of the Chloroplast-encoded Gene matK
… proposed chloroplast-encoded group II intron maturase. There are 15 genes in the chloroplast that would require a maturase for RNA splicing. Six of these genes have introns that are not excised by a nuclear imported maturase, leaving MatK as the only candidate for processing introns in these …
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Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae
… on coordination between ymLeuRS and bI4 maturase. An in vitro splicing assay has been established that is based on assembly of proteins into a competent ternary splicing complex. RNA deletion mutants have been quantitated for effects on complex assembly and splicing activity.
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Mechanisms of leucyl-tRNA synthetase dependent group I intron splicing
… respiratory genes. The LeuRS collaborates with a maturase to excise the bI4 and aI4α introns from the cob and cox1α genes respectively. The LeuRS-based suppressor mutations have been isolated within the amino acid editing CP1 domain and restore native RNA splicing activity in the presence of an …
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DNA barcoding of Jatropha curcas
… ,5-bisphosphate carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and Arabidopsis thaliana. I found that the rbcL gene in J. cureas and the 3' end of L. sativa met the …
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Biohydrogen as a Fuel: Understanding and Engineering Hydrogenase Enzymes for Biotechnological Applications
… it was found that the native E. coli maturase system, critical for biosynthesis of the [NiFe] cofactor, could assemble a functional enzyme. The converse was also found: that the C. necator maturase operons were able to complement E. coli mutants defective in hydrogenase …
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Structural Studies of Three Factors That Affect the Prokaryotic Transcription Cycle; Microcin J25, LAMBDA Q and T4 GP33
… and post-translationally modified by two maturase enzymes. We synthesized McjA and constructed a protein-A tagged biosynthesis cassette to be used in the analysis of MccJ25 maturation. Escherichia coli (Ec) RNAP has not, thus far, been amenable to crystallization despite considerable …
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Authentication and investigation of potential hepatotoxicity of Black Cohosh
… (nrITS) was an ideal barcoding region and that maturase K (MatK) was a poor choice for Actaea species. To address the issue of hepatotoxicity claims, cultured human hepatocyte derived cells were treated with 60% ethanol extracts of Actaea racemosa and Asian Actaea. A qPCR array was utilised to …
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Molekular-Systematische Untersuchungen an den Familien Nepenthaceae und Ancistrocladaceae sowie verwandter Taxa aus der Unterklasse Caryophyllidae s. l.
… Unterbrechung des Leserasters im f?xFC;r die Maturase K kodierenden Bereich des trnK-Introns (matK) ein Pseudogen darstellt, wurde als zus?xE4;tzlicher phylogenetischer Marker f?xFC;r die Nepenthaceae vergleichend sequenziert, eignete sich aber nur eingeschr?xE4;nkt zur phylogenetischen …
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The Phylogenetic Reconstruction of the Grass Family (Poaceae) Using matK Gene Sequences
Comparative DNA sequencing of matK, a maturase-encoding gene located within the intron of the chloroplast trnK gene, was evaluated for phylogenetic utility above the family level and within the grass family (Poaceae). There are three major objectives in the research. The first one is to study the …